Reviewed, UniProtKB/Swiss-Prot Q8XH79 (MURA2_CLOPE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Synonyms:	murZ
Ordered Locus Names:	CPE2606

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000178866
Sites
	Active site	118	1	Proton donor By similarity
	Binding site	118	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XH79-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 88C3E3CB0D600188	FASTA	417	44,533
	10 20 30 40 50 60 MNRLIINGGK ALKGSIEING AKNAAVAILP AAILASKGEC IIDNVPDIAD VHCLERIIRS 70 80 90 100 110 120 LGCNVEKLDN NTLKINAEEI KTVEACGNDV RKMRASYYFI GALLARFKEA KVELPGGCPI 130 140 150 160 170 180 GVRPIDQHIK GFEALGAKVS IEHGAVNIMA EKLIGTNIFF DVVSVGATIN LMIAATLAEG 190 200 210 220 230 240 TTVLENAARE PHVVDVANFL NAMGANVKGA GTDVIRIKGV KELKGCNYSV VPDQIEAGTF 250 260 270 280 290 300 MIAAAATRGD VTIQNVIPKH LESISAKLLE MGAKVEEGDD SVRVYVDGDL KGVNLKTAPY 310 320 330 340 350 360 PGFPTDVQQP MCTLLSTAKG RSIIVETIWE NRHKHVDELK KMGATIKVEG RSAIIDGVDR 370 380 390 400 410 LTGAVVKATD LRAGAAMVIA GLISDGVTEI TSIEHIDRGY PHIEEKFRML GADIVRK

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB82312.1.
RefSeq	NP_563522.1.
3D structure databases
HSSP	HSSP built from PDB template 1DLG based on UniProtKB P33038.
ModBase	Search...
Genome annotation databases
GeneID	990983.
GenomeReviews	Gene locus CPE2606 in contig BA000016_GR.
KEGG	cpe:CPE2606.
NMPDR	fig|195102.1.peg.2669.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XH79.
OMA	IDNIPDI.
Enzyme and pathway databases
BioCyc	CPER195102:CPE2606-MON.
BRENDA	2.5.1.7. 2406.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_CLOPE

Accession

Primary (citable) accession number: Q8XH79

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)