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Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).By similarity

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathway: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131Proton acceptorUniRule annotation
Metal bindingi13 – 131MagnesiumUniRule annotation
Metal bindingi40 – 401Magnesium; via carbonyl oxygenUniRule annotation
Active sitei41 – 411Proton donorUniRule annotation
Binding sitei130 – 1301IMPUniRule annotation
Binding sitei144 – 1441IMP; shared with dimeric partnerUniRule annotation
Binding sitei224 – 2241IMPUniRule annotation
Binding sitei239 – 2391IMPUniRule annotation
Binding sitei303 – 3031IMPUniRule annotation
Binding sitei305 – 3051GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187GTPUniRule annotation
Nucleotide bindingi40 – 423GTPUniRule annotation
Nucleotide bindingi331 – 3333GTPUniRule annotation
Nucleotide bindingi413 – 4153GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195102:GJFM-2753-MONOMER.
UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
Gene namesi
Name:purAUniRule annotation
Ordered Locus Names:CPE2622
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Adenylosuccinate synthetasePRO_0000095168Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8XH63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 164IMP bindingUniRule annotation
Regioni38 – 414IMP bindingUniRule annotation
Regioni299 – 3057Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0104.
HOGENOMiHOG000260959.
KOiK01939.
OMAiFVVRYQG.
OrthoDBiEOG68Q0QG.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
InterProiIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XH63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFVVLGAQ WGDEGKGKMT DYLAEEAEVV VRFQGGNNAG HTVEVEDKQY
60 70 80 90 100
KLHLIPSGIL HDEKLNVIGN GVVVDPKALF TEIDYLEGLG VKVTPEKLIV
110 120 130 140 150
SDRAHLIMPY HITLDKLKEK ARGKNDIGTT CKGIGPCYTD KYERSGIRVC
160 170 180 190 200
DLMHKDSFAE KLRINIEMKN GYIKLLGGEE LNFDEIYNEY MAFAERLRPY
210 220 230 240 250
VKDTSVEIYN AIQADKNVLF EGAQGMLLDI DYGTYPYVTS SNTTSCGVAS
260 270 280 290 300
GAGIGPNMVT NAVGIAKAYT TRVGKGPFPT ELENETGDWI REKGHEYGVT
310 320 330 340 350
TGRSRRCGWL DLVILKTTTR VCGLTSLVVT KIDTLAGLDK IQMCVGYELD
360 370 380 390 400
GKVIDYFPAS LEDLARCKPV YEEFEGWGEE VADARSYEEL PENAKTYLRR
410 420
IEEFTGTKVS IVGVGPKRNQ TIRVREEL
Length:428
Mass (Da):47,590
Last modified:March 1, 2002 - v1
Checksum:i7323E7092EF0462B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB82328.1.
RefSeqiNP_563538.1. NC_003366.1.
WP_003450991.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB82328; BAB82328; BAB82328.
GeneIDi989276.
KEGGicpe:CPE2622.
PATRICi19499357. VBICloPer59675_2693.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB82328.1.
RefSeqiNP_563538.1. NC_003366.1.
WP_003450991.1. NC_003366.1.

3D structure databases

ProteinModelPortaliQ8XH63.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB82328; BAB82328; BAB82328.
GeneIDi989276.
KEGGicpe:CPE2622.
PATRICi19499357. VBICloPer59675_2693.

Phylogenomic databases

eggNOGiCOG0104.
HOGENOMiHOG000260959.
KOiK01939.
OMAiFVVRYQG.
OrthoDBiEOG68Q0QG.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.
BioCyciCPER195102:GJFM-2753-MONOMER.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
InterProiIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiPURA_CLOPE
AccessioniPrimary (citable) accession number: Q8XH63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.