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Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.UniRule annotation

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13Proton acceptorUniRule annotation1
Metal bindingi13MagnesiumUniRule annotation1
Metal bindingi40Magnesium; via carbonyl oxygenUniRule annotation1
Active sitei41Proton donorUniRule annotation1
Binding sitei130IMPUniRule annotation1
Binding sitei144IMP; shared with dimeric partnerUniRule annotation1
Binding sitei224IMPUniRule annotation1
Binding sitei239IMPUniRule annotation1
Binding sitei303IMPUniRule annotation1
Binding sitei305GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18GTPUniRule annotation7
Nucleotide bindingi40 – 42GTPUniRule annotation3
Nucleotide bindingi331 – 333GTPUniRule annotation3
Nucleotide bindingi413 – 415GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
Gene namesi
Name:purAUniRule annotation
Ordered Locus Names:CPE2622
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000951681 – 428Adenylosuccinate synthetaseAdd BLAST428

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8XH63.
SMRiQ8XH63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 16IMP bindingUniRule annotation4
Regioni38 – 41IMP bindingUniRule annotation4
Regioni299 – 305Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260959.
KOiK01939.
OMAiFHHAKPI.
OrthoDBiPOG091H01G9.

Family and domain databases

CDDicd03108. AdSS. 1 hit.
HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XH63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFVVLGAQ WGDEGKGKMT DYLAEEAEVV VRFQGGNNAG HTVEVEDKQY
60 70 80 90 100
KLHLIPSGIL HDEKLNVIGN GVVVDPKALF TEIDYLEGLG VKVTPEKLIV
110 120 130 140 150
SDRAHLIMPY HITLDKLKEK ARGKNDIGTT CKGIGPCYTD KYERSGIRVC
160 170 180 190 200
DLMHKDSFAE KLRINIEMKN GYIKLLGGEE LNFDEIYNEY MAFAERLRPY
210 220 230 240 250
VKDTSVEIYN AIQADKNVLF EGAQGMLLDI DYGTYPYVTS SNTTSCGVAS
260 270 280 290 300
GAGIGPNMVT NAVGIAKAYT TRVGKGPFPT ELENETGDWI REKGHEYGVT
310 320 330 340 350
TGRSRRCGWL DLVILKTTTR VCGLTSLVVT KIDTLAGLDK IQMCVGYELD
360 370 380 390 400
GKVIDYFPAS LEDLARCKPV YEEFEGWGEE VADARSYEEL PENAKTYLRR
410 420
IEEFTGTKVS IVGVGPKRNQ TIRVREEL
Length:428
Mass (Da):47,590
Last modified:March 1, 2002 - v1
Checksum:i7323E7092EF0462B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB82328.1.
RefSeqiWP_003450991.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB82328; BAB82328; BAB82328.
KEGGicpe:CPE2622.
PATRICi19499357. VBICloPer59675_2693.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB82328.1.
RefSeqiWP_003450991.1. NC_003366.1.

3D structure databases

ProteinModelPortaliQ8XH63.
SMRiQ8XH63.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB82328; BAB82328; BAB82328.
KEGGicpe:CPE2622.
PATRICi19499357. VBICloPer59675_2693.

Phylogenomic databases

HOGENOMiHOG000260959.
KOiK01939.
OMAiFHHAKPI.
OrthoDBiPOG091H01G9.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.

Family and domain databases

CDDicd03108. AdSS. 1 hit.
HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURA_CLOPE
AccessioniPrimary (citable) accession number: Q8XH63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.