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Q8XH63

- PURA_CLOPE

UniProt

Q8XH63 - PURA_CLOPE

Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP By similarity.By similarity

    Catalytic activityi

    GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei13 – 131Proton acceptorUniRule annotation
    Metal bindingi13 – 131MagnesiumUniRule annotation
    Metal bindingi40 – 401Magnesium; via carbonyl oxygenUniRule annotation
    Active sitei41 – 411Proton donorUniRule annotation
    Binding sitei130 – 1301IMPUniRule annotation
    Binding sitei144 – 1441IMP; shared with dimeric partnerUniRule annotation
    Binding sitei224 – 2241IMPUniRule annotation
    Binding sitei239 – 2391IMPUniRule annotation
    Binding sitei303 – 3031IMPUniRule annotation
    Binding sitei305 – 3051GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 187GTPUniRule annotation
    Nucleotide bindingi40 – 423GTPUniRule annotation
    Nucleotide bindingi331 – 3333GTPUniRule annotation
    Nucleotide bindingi413 – 4153GTPUniRule annotation

    GO - Molecular functioni

    1. adenylosuccinate synthase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' AMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCPER195102:GJFM-2753-MONOMER.
    UniPathwayiUPA00075; UER00335.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
    Short name:
    AMPSaseUniRule annotation
    Short name:
    AdSSUniRule annotation
    Alternative name(s):
    IMP--aspartate ligaseUniRule annotation
    Gene namesi
    Name:purAUniRule annotation
    Ordered Locus Names:CPE2622
    OrganismiClostridium perfringens (strain 13 / Type A)
    Taxonomic identifieri195102 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000818: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Adenylosuccinate synthetasePRO_0000095168Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi195102.CPE2622.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8XH63.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 164IMP bindingUniRule annotation
    Regioni38 – 414IMP bindingUniRule annotation
    Regioni299 – 3057Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the adenylosuccinate synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0104.
    HOGENOMiHOG000260959.
    KOiK01939.
    OMAiISEACPI.
    OrthoDBiEOG68Q0QG.

    Family and domain databases

    HAMAPiMF_00011. Adenylosucc_synth.
    InterProiIPR018220. Adenylosuccinate_synthase_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11846. PTHR11846. 1 hit.
    PfamiPF00709. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SMARTiSM00788. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00184. purA. 1 hit.
    PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8XH63-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAFVVLGAQ WGDEGKGKMT DYLAEEAEVV VRFQGGNNAG HTVEVEDKQY    50
    KLHLIPSGIL HDEKLNVIGN GVVVDPKALF TEIDYLEGLG VKVTPEKLIV 100
    SDRAHLIMPY HITLDKLKEK ARGKNDIGTT CKGIGPCYTD KYERSGIRVC 150
    DLMHKDSFAE KLRINIEMKN GYIKLLGGEE LNFDEIYNEY MAFAERLRPY 200
    VKDTSVEIYN AIQADKNVLF EGAQGMLLDI DYGTYPYVTS SNTTSCGVAS 250
    GAGIGPNMVT NAVGIAKAYT TRVGKGPFPT ELENETGDWI REKGHEYGVT 300
    TGRSRRCGWL DLVILKTTTR VCGLTSLVVT KIDTLAGLDK IQMCVGYELD 350
    GKVIDYFPAS LEDLARCKPV YEEFEGWGEE VADARSYEEL PENAKTYLRR 400
    IEEFTGTKVS IVGVGPKRNQ TIRVREEL 428
    Length:428
    Mass (Da):47,590
    Last modified:March 1, 2002 - v1
    Checksum:i7323E7092EF0462B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB82328.1.
    RefSeqiNP_563538.1. NC_003366.1.

    Genome annotation databases

    EnsemblBacteriaiBAB82328; BAB82328; BAB82328.
    GeneIDi989276.
    KEGGicpe:CPE2622.
    PATRICi19499357. VBICloPer59675_2693.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB82328.1 .
    RefSeqi NP_563538.1. NC_003366.1.

    3D structure databases

    ProteinModelPortali Q8XH63.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 195102.CPE2622.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB82328 ; BAB82328 ; BAB82328 .
    GeneIDi 989276.
    KEGGi cpe:CPE2622.
    PATRICi 19499357. VBICloPer59675_2693.

    Phylogenomic databases

    eggNOGi COG0104.
    HOGENOMi HOG000260959.
    KOi K01939.
    OMAi ISEACPI.
    OrthoDBi EOG68Q0QG.

    Enzyme and pathway databases

    UniPathwayi UPA00075 ; UER00335 .
    BioCyci CPER195102:GJFM-2753-MONOMER.

    Family and domain databases

    HAMAPi MF_00011. Adenylosucc_synth.
    InterProi IPR018220. Adenylosuccinate_synthase_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11846. PTHR11846. 1 hit.
    Pfami PF00709. Adenylsucc_synt. 1 hit.
    [Graphical view ]
    SMARTi SM00788. Adenylsucc_synt. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00184. purA. 1 hit.
    PROSITEi PS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13 / Type A.

    Entry informationi

    Entry nameiPURA_CLOPE
    AccessioniPrimary (citable) accession number: Q8XH63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3