Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8XH63

- PURA_CLOPE

UniProt

Q8XH63 - PURA_CLOPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).By similarity

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131Proton acceptorUniRule annotation
Metal bindingi13 – 131MagnesiumUniRule annotation
Metal bindingi40 – 401Magnesium; via carbonyl oxygenUniRule annotation
Active sitei41 – 411Proton donorUniRule annotation
Binding sitei130 – 1301IMPUniRule annotation
Binding sitei144 – 1441IMP; shared with dimeric partnerUniRule annotation
Binding sitei224 – 2241IMPUniRule annotation
Binding sitei239 – 2391IMPUniRule annotation
Binding sitei303 – 3031IMPUniRule annotation
Binding sitei305 – 3051GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187GTPUniRule annotation
Nucleotide bindingi40 – 423GTPUniRule annotation
Nucleotide bindingi331 – 3333GTPUniRule annotation
Nucleotide bindingi413 – 4153GTPUniRule annotation

GO - Molecular functioni

  1. adenylosuccinate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' AMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195102:GJFM-2753-MONOMER.
UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
Gene namesi
Name:purAUniRule annotation
Ordered Locus Names:CPE2622
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Adenylosuccinate synthetasePRO_0000095168Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi195102.CPE2622.

Structurei

3D structure databases

ProteinModelPortaliQ8XH63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 164IMP bindingUniRule annotation
Regioni38 – 414IMP bindingUniRule annotation
Regioni299 – 3057Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0104.
HOGENOMiHOG000260959.
KOiK01939.
OMAiISEACPI.
OrthoDBiEOG68Q0QG.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
InterProiIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XH63-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAFVVLGAQ WGDEGKGKMT DYLAEEAEVV VRFQGGNNAG HTVEVEDKQY
60 70 80 90 100
KLHLIPSGIL HDEKLNVIGN GVVVDPKALF TEIDYLEGLG VKVTPEKLIV
110 120 130 140 150
SDRAHLIMPY HITLDKLKEK ARGKNDIGTT CKGIGPCYTD KYERSGIRVC
160 170 180 190 200
DLMHKDSFAE KLRINIEMKN GYIKLLGGEE LNFDEIYNEY MAFAERLRPY
210 220 230 240 250
VKDTSVEIYN AIQADKNVLF EGAQGMLLDI DYGTYPYVTS SNTTSCGVAS
260 270 280 290 300
GAGIGPNMVT NAVGIAKAYT TRVGKGPFPT ELENETGDWI REKGHEYGVT
310 320 330 340 350
TGRSRRCGWL DLVILKTTTR VCGLTSLVVT KIDTLAGLDK IQMCVGYELD
360 370 380 390 400
GKVIDYFPAS LEDLARCKPV YEEFEGWGEE VADARSYEEL PENAKTYLRR
410 420
IEEFTGTKVS IVGVGPKRNQ TIRVREEL
Length:428
Mass (Da):47,590
Last modified:March 1, 2002 - v1
Checksum:i7323E7092EF0462B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB82328.1.
RefSeqiNP_563538.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB82328; BAB82328; BAB82328.
GeneIDi989276.
KEGGicpe:CPE2622.
PATRICi19499357. VBICloPer59675_2693.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB82328.1 .
RefSeqi NP_563538.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q8XH63.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE2622.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB82328 ; BAB82328 ; BAB82328 .
GeneIDi 989276.
KEGGi cpe:CPE2622.
PATRICi 19499357. VBICloPer59675_2693.

Phylogenomic databases

eggNOGi COG0104.
HOGENOMi HOG000260959.
KOi K01939.
OMAi ISEACPI.
OrthoDBi EOG68Q0QG.

Enzyme and pathway databases

UniPathwayi UPA00075 ; UER00335 .
BioCyci CPER195102:GJFM-2753-MONOMER.

Family and domain databases

HAMAPi MF_00011. Adenylosucc_synth.
InterProi IPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11846. PTHR11846. 1 hit.
Pfami PF00709. Adenylsucc_synt. 1 hit.
[Graphical view ]
SMARTi SM00788. Adenylsucc_synt. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00184. purA. 1 hit.
PROSITEi PS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiPURA_CLOPE
AccessioniPrimary (citable) accession number: Q8XH63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3