Reviewed, UniProtKB/Swiss-Prot Q8XEC3 (UBIC_ECO57)

Names and origin

Protein names

Recommended name:
    Chorismate--pyruvate lyase
      Short name=CL
      Short name=CPL
    EC=4.1.3.40

Gene names

Name:	ubiC
Ordered Locus Names:	Z5638, ECs5022

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway By similarity.
Catalytic activity	Chorismate = 4-hydroxybenzoate + pyruvate. HAMAP MF_01632
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP MF_01632
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ubiC family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Cellular component	Cytoplasm
Ligand	Pyruvate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyruvate biosynthetic process Inferred from electronic annotation. Source: HAMAP ubiquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chorismate lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Chorismate--pyruvate lyase HAMAP MF_01632		PRO_0000240546
Sites
	Binding site	35	1	Substrate; via amide nitrogen By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	115	1	Substrate; via amide nitrogen By similarity
Experimental info
	Sequence conflict	161	1	A → T in BAB38445. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8XEC3-1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: A6CA1655BC325479	FASTA	165	18,791
	10 20 30 40 50 60 MSHPALTQLR ALRYCKEIPA LEPQLLDWLL LEDSMTKRFE QQGKTVSVTM IREGFVEQNE 70 80 90 100 110 120 IPEELPLLPK ESRYWLREIL LCADGEPWLA GRTVVPVSTL SGPELALQKL GKTPLGRYLF 130 140 150 160 TSSTLTRDFI EIGRDAGLWG RRSRLRLSGK PLLLTELFLP ASPLY

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG59238.1. Different initiation. BA000007 Genomic DNA. Translation: BAB38445.1. Different initiation.
PIR	B86097. F91256.
RefSeq	NP_290673.2. NP_313049.2.
3D structure databases
HSSP	HSSP built from PDB template 1G81 based on UniProtKB P26602.
SMR	Q8XEC3. Positions 2-165.
ModBase	Search...
Genome annotation databases
GeneID	914312. 960084.
GenomeReviews	Gene locus Z5638 in contig AE005174_GR. Gene locus ECs5022 in contig BA000007_GR.
KEGG	ece:Z5638. ecs:ECs5022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XEC3.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS5022-MON.
Family and domain databases
HAMAP	MF_01632. [Tree]
InterPro	IPR007440. Chorismate--pyruvate_lyase. [Graphical view]
Pfam	PF04345. Chor_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIC_ECO57

Accession

Primary (citable) accession number: Q8XEC3
Secondary accession number(s): Q7A934

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	September 22, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)