Reviewed, UniProtKB/Swiss-Prot Q8XE72 (PANE_ECO57)

Names and origin

Protein names

Recommended name:
    2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Name:	panE
Synonyms:	abpA
Ordered Locus Names:	Z0528, ECs0479

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	2-dehydropantoate 2-reductase		PRO_0000157302
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	176	1	Proton donor
	Binding site	31	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	122	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	256	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XE72-1 [UniParc]. Last modified February 21, 2002. Version 1. Checksum: 13D127AEF0E30C64	FASTA	303	33,848
	10 20 30 40 50 60 MKITVLGCGA LGQLWLTALC KQGHEVQGWL RVPQPYCSVN LVETDGSIFN ESLTANDPDF 70 80 90 100 110 120 LATSDLLLVT LKAWQVSDAV KSLASTLPVT TPILLIHNGM GTIEELQNIQ QPLLMGTTTH 130 140 150 160 170 180 AACRDGNVII HVANGITHIG PARQQDGDYS YLADILQTVL PDVAWHNNIR AELWRKLAVN 190 200 210 220 230 240 CVINPLTAIW NCPNGELRHH PQEIMQICEE VAAVIEREGH HTSAEDLRDY VMQVIDATAE 250 260 270 280 290 300 NISSMLQDIR TLRHTEIDYI NGFLLRRARA HGIAVPENTR LFEMVKRKES EYERIGTGLP RPW

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54775.1. BA000007 Genomic DNA. Translation: BAB33902.1.
PIR	C85539. G90688.
RefSeq	NP_286167.1. NP_308506.1.
3D structure databases
SMR	Q8XE72. Positions 1-293.
ModBase	Search...
Genome annotation databases
GeneID	914581. 957394.
GenomeReviews	Gene locus Z0528 in contig AE005174_GR. Gene locus ECs0479 in contig BA000007_GR.
KEGG	ece:Z0528. ecs:ECs0479.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668370.
OMA	QCHILLL.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0479-MONOMER.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_ECO57

Accession

Primary (citable) accession number: Q8XE72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)