Q8XE58 (CCMA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c biogenesis ATP-binding export protein CcmA
EC=3.6.3.41

Alternative name(s):
Heme exporter protein A

Gene names

Name:	ccmA
Ordered Locus Names:	Z3458, ECs3090

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex CcmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system By similarity. HAMAP MF_01707
Catalytic activity	ATP + H2O + heme(In) = ADP + phosphate + heme(Out). HAMAP MF_01707
Subunit structure	The complex is composed of two ATP-binding proteins (CcmA) and two transmembrane proteins (CcmB) By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP MF_01707.
Sequence similarities	Belongs to the ABC transporter superfamily. CcmA exporter (TC 3.A.1.107) family. [View classification] Contains 1 ABC transporter domain.
Sequence caution	The sequence AAG57336.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB36513.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW heme-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Cytochrome c biogenesis ATP-binding export protein CcmA HAMAP MF_01707		PRO_0000092178
Regions
	Domain	4 – 207	204	ABC transporter
	Nucleotide binding	36 – 43	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8XE58 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 748F3CC1E2179291	FASTA	207	23,109
	10 20 30 40 50 60 MGMLEVRELL CERDERTLFS GLSFTLNAGE WVQITGSNGA GKTTLLRLLT GLSRPDAGEV 70 80 90 100 110 120 LWQGQPLHQV RDSYHQNLLW IGHQPGIKTR LTALENLHFY HRDGDTAQCL EALAQAGLAG 130 140 150 160 170 180 FEDIPVNQLS AGQQRRVALA RLWLTRATLW ILDEPFTAID VNGVDRLTQR MAQHTEQGGI 190 200 VILTTHQPLN VAESKIRRIS LTQTRAV

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57336.1. Different initiation. BA000007 Genomic DNA. Translation: BAB36513.1. Different initiation.
PIR	B91015. D85859.
RefSeq	NP_288781.1. NC_002655.2. NP_311117.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8XE58.
SMR	Q8XE58. Positions 4-207.
ModBase	Search...
Protein family/group databases
TCDB	3.A.1.107.3. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000026591; EBESCP00000025484; EBESCG00000025644. EBESCT00000059072; EBESCP00000056900; EBESCG00000058120.
GeneID	916796. 957178.
GenomeReviews	Gene locus Z3458 in contig AE005174_GR. Gene locus ECs3090 in contig BA000007_GR.
KEGG	ece:Z3458. ecs:ECs3090.
PATRIC	18355564. VBIEscCol44059_2983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008239.
HOGENOM	HBG758042.
OMA	PFNAIDK.
ProtClustDB	PRK13538.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3090-MONOMER.
Family and domain databases
HAMAP	MF_01707. ABC_ATPase_CcmA. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR005895. ABC_transptr_haem_export_CcmA. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K02193.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01189. CcmA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51243. CCMA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCMA_ECO57

Accession

Primary (citable) accession number: Q8XE58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program