Q8XDR6 (LCFA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase
EC=6.2.1.3

Alternative name(s):
Long-chain acyl-CoA synthetase
Short name=Acyl-CoA synthetase

Gene names

Name:	fadD
Synonyms:	oldD
Ordered Locus Names:	Z2848, ECs2514

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.
Catalytic activity	ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Subunit structure	Homodimer Probable.
Subcellular location	Membrane; Peripheral membrane protein Potential. Note: Partially membrane-associated Potential.
Miscellaneous	Activity is the highest with fatty acid substrates of > 10 carbon atoms By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	long-chain fatty acid metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Long-chain-fatty-acid--CoA ligase		PRO_0000193127
Regions
	Nucleotide binding	213 – 224	12	ATP Probable

Sequences

	Sequence		Length	Mass (Da)
	Q8XDR6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4DE944AB7DF40CF2	FASTA	561	62,364
	10 20 30 40 50 60 MKKVWLNRYP ADVPTEINPD RYQSLVDMFE QSVARYADQP AFVNMGEVMT FRKLEERSRA 70 80 90 100 110 120 FAAYLQQGLG LKKGDRVALM MPNLLQYPVA LFGILRAGMI VVNVNPLYTP RELEHQLNDS 130 140 150 160 170 180 GASAIVIVSN FAHTLEKVVD KTAVQHVILT RMGDQLSTAK GTLVNFVVKY IKRLVPKYHL 190 200 210 220 230 240 PDAISFRSAL HNGYRMQYVK PELVPEDLAF LQYTGGTTGV AKGAMLTHRN MLANLEQVNA 250 260 270 280 290 300 TYGPLLHPGK ELVVTALPLY HIFALTINCL LFIELGGQNL LITNPRDIPG LVKELAKYPF 310 320 330 340 350 360 TAITGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQQV VAERWVKLTG QYLLEGYGLT 370 380 390 400 410 420 ECAPLVSVNP YDIDYHSGSI GLPVPSTEAK LVDDDDNEVS PGQPGELCVR GPQVMLGYWQ 430 440 450 460 470 480 RPDATDEIIK NGWLHTGDIA VMDEEGFLRI VDRKKDMILV SGFNVYPNEI EDVVMQHPGV 490 500 510 520 530 540 QEVAAVGVPS GSSGEAVKIF VVKKDPSLTE ESLVTFCRRQ LTGYKVPKLV EFRDELPKSN 550 560 VGKILRRELR DEARGKVDNK A

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56794.1. BA000007 Genomic DNA. Translation: BAB35937.1.
PIR	B90943. F85791.
RefSeq	NP_288241.1. NC_002655.2. NP_310541.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8XDR6.
SMR	Q8XDR6. Positions 23-551.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z2848.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG56794; AAG56794; Z2848. BAB35937; BAB35937; BAB35937.
GeneID	912449. 961778.
KEGG	ece:Z2848. ecs:ECs2514.
PATRIC	18354390. VBIEscCol44059_2406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0318.
HOGENOM	HOG000229983.
KO	K01897.
OMA	MPVQQAV.
ProtClustDB	PRK08974.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-2487-MONOMER.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR025110. DUF4009. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF13193. DUF4009. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCFA_ECO57

Accession

Primary (citable) accession number: Q8XDR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program