Reviewed, UniProtKB/Swiss-Prot Q8XDG9 (MTLD_ECO57)

Names and origin

Protein names

Recommended name:
    Mannitol-1-phosphate 5-dehydrogenase
    EC=1.1.1.17

Gene names

Name:	mtlD
Ordered Locus Names:	Z5024, ECs4476

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-mannitol 1-phosphate + NAD(+) = D-fructose 6-phosphate + NADH.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Mannitol-1-phosphate 5-dehydrogenase		PRO_0000170705
Regions
	Nucleotide binding	3 – 14	12	NAD By similarity
Amino acid modifications
	Modified residue	269	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XDG9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E38BB1867C53CA3C	FASTA	382	41,198
	10 20 30 40 50 60 MKALHFGAGN IGRGFIGKLL ADAGIQLTFA DVNQVVLDAL NARHSYQVHV VGETEQVDTV 70 80 90 100 110 120 SGVDAVSSIG DDVVDLIAQV DLVTTAVGPV VLERIAPAIA KGLVKRKEQG NESPLNIIAC 130 140 150 160 170 180 ENMVRGTTQL KGHVMNALPE DAKAWVEEHV GFVDSAVDRI VPPSASATND PLEVTVETFS 190 200 210 220 230 240 EWIVDKTQFK GALPNIPGME LTDNLMAFVE RKLFTLNTGH AITAYLGKLA GHQTIRDAIL 250 260 270 280 290 300 DEKIRAVVKG AMEESGAVLI KRYGFDADKH AAYIQKILGR FENPYLKDDV ERVGRQPLRK 310 320 330 340 350 360 LSAGDRLIKP LLGTLEYSLP HKNLIQGIAG AMHFRSEDDP QAQELAALIA DKGPQAALAQ 370 380 ISDLDANSEV VSEAVTAYKA MQ

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG58744.1. BA000007 Genomic DNA. Translation: BAB37899.1.
PIR	D86035. D91188.
RefSeq	NP_290180.1. NP_312503.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	915582. 960952.
GenomeReviews	Gene locus ECs4476 in contig BA000007_GR. Gene locus Z5024 in contig AE005174_GR.
KEGG	ece:Z5024. ecs:ECs4476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XDG9.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4476-MON.
Family and domain databases
HAMAP	MF_00196. [Tree]
InterPro	IPR013328. DHase_multihelical. IPR013118. Mannitol_DHase_C. IPR000669. Mannitol_DHase_core. IPR013131. Mannitol_DHase_N. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

MTLD_ECO57

Accession

Primary (citable) accession number: Q8XDG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	November 4, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)