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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi415Magnesium 1UniRule annotation1
Metal bindingi422Magnesium 1UniRule annotation1
Metal bindingi422Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligaseUniRule annotation (EC:6.1.1.6UniRule annotation)
Alternative name(s):
Lysyl-tRNA synthetaseUniRule annotation
Short name:
LysRSUniRule annotation
Gene namesi
Name:lysSUniRule annotation
Ordered Locus Names:Z4228, ECs3762
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001526242 – 505Lysine--tRNA ligaseAdd BLAST504

Proteomic databases

PRIDEiQ8XD57

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z4228

Structurei

3D structure databases

ProteinModelPortaliQ8XD57
SMRiQ8XD57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CRK Bacteria
COG1190 LUCA
HOGENOMiHOG000236578
KOiK04567
OMAiMMNIIPG

Family and domain databases

CDDicd00775 LysRS_core, 1 hit
HAMAPiMF_00252 Lys_tRNA_synth_class2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR002313 Lys-tRNA-ligase_II
IPR034762 Lys-tRNA-ligase_II_bac/euk
IPR018149 Lys-tRNA-synth_II_C
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PIRSFiPIRSF039101 LysRS2, 1 hit
PRINTSiPR00982 TRNASYNTHLYS
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00499 lysS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XD57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQHAQGAD AVVDLNNELK TRREKLANLR EQGIAFPNDF RRDHTSDQLH
60 70 80 90 100
AEFDGKENEE LEALNIEVAV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA
110 120 130 140 150
RDDLPEGVYN EQFKKWDLGD ILGAKGKLFK TKTGELSIHC TELRLLTKAL
160 170 180 190 200
RPLPDKFHGL QDQEARYRQR YLDLISNDES RNTFKVRSQI LSGIRQFMVN
210 220 230 240 250
RGFMEVETPM MQVIPGGAAA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG
260 270 280 290 300
GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYKDL IELTESLFRT
310 320 330 340 350
LAQDILGKTE VTYGDVTLDF GKPFEKLTMR EAIKKYRPET DMADLDNFDS
360 370 380 390 400
AKAIVESIGI HVEKSWGLGR IVTEIFEEVA EAHLIQPTFI TEYPAEVSPL
410 420 430 440 450
ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAQRF LDQVAAKDAG
460 470 480 490 500
DDEAMFYDED YVTALEHGLP PTAGLGIGID RMVMLFTNSH TIRDVILFPA

MRPVK
Length:505
Mass (Da):57,631
Last modified:January 23, 2007 - v3
Checksum:iCDABF71FAC12A629
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58018.1
BA000007 Genomic DNA Translation: BAB37185.1
PIRiB91099
F85944
RefSeqiNP_311789.1, NC_002695.1
WP_000003075.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58018; AAG58018; Z4228
BAB37185; BAB37185; BAB37185
GeneIDi916412
KEGGiece:Z4228
ecs:ECs3762
PATRICifig|386585.9.peg.3925

Similar proteinsi

Entry informationi

Entry nameiSYK1_ECO57
AccessioniPrimary (citable) accession number: Q8XD57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 114 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health