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Q8XD57

- SYK1_ECO57

UniProt

Q8XD57 - SYK1_ECO57

Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).UniRule annotation

    Cofactori

    Binds 3 magnesium ions per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi415 – 4151Magnesium 1UniRule annotation
    Metal bindingi422 – 4221Magnesium 1UniRule annotation
    Metal bindingi422 – 4221Magnesium 2UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. lysine-tRNA ligase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. lysyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-3729-MONOMER.
    ECOO157:LYSS-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysine--tRNA ligaseUniRule annotation (EC:6.1.1.6UniRule annotation)
    Alternative name(s):
    Lysyl-tRNA synthetaseUniRule annotation
    Short name:
    LysRSUniRule annotation
    Gene namesi
    Name:lysSUniRule annotation
    Ordered Locus Names:Z4228, ECs3762
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 505504Lysine--tRNA ligasePRO_0000152624Add
    BLAST

    Proteomic databases

    PRIDEiQ8XD57.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi155864.Z4228.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8XD57.
    SMRiQ8XD57. Positions 12-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1190.
    HOGENOMiHOG000236578.
    KOiK04567.
    OMAiGTEICLE.
    OrthoDBiEOG69PQ2M.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00252. Lys_tRNA_synth_class2.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR002313. Lys-tRNA-ligase_II.
    IPR018149. Lys-tRNA-synth_II_C.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PfamiPF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR00982. TRNASYNTHLYS.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8XD57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEQHAQGAD AVVDLNNELK TRREKLANLR EQGIAFPNDF RRDHTSDQLH    50
    AEFDGKENEE LEALNIEVAV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA 100
    RDDLPEGVYN EQFKKWDLGD ILGAKGKLFK TKTGELSIHC TELRLLTKAL 150
    RPLPDKFHGL QDQEARYRQR YLDLISNDES RNTFKVRSQI LSGIRQFMVN 200
    RGFMEVETPM MQVIPGGAAA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG 250
    GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYKDL IELTESLFRT 300
    LAQDILGKTE VTYGDVTLDF GKPFEKLTMR EAIKKYRPET DMADLDNFDS 350
    AKAIVESIGI HVEKSWGLGR IVTEIFEEVA EAHLIQPTFI TEYPAEVSPL 400
    ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAQRF LDQVAAKDAG 450
    DDEAMFYDED YVTALEHGLP PTAGLGIGID RMVMLFTNSH TIRDVILFPA 500
    MRPVK 505
    Length:505
    Mass (Da):57,631
    Last modified:January 23, 2007 - v3
    Checksum:iCDABF71FAC12A629
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58018.1.
    BA000007 Genomic DNA. Translation: BAB37185.1.
    PIRiB91099.
    F85944.
    RefSeqiNP_289459.1. NC_002655.2.
    NP_311789.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG58018; AAG58018; Z4228.
    BAB37185; BAB37185; BAB37185.
    GeneIDi916412.
    958385.
    KEGGiece:Z4228.
    ecs:ECs3762.
    PATRICi18357007. VBIEscCol44059_3682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58018.1 .
    BA000007 Genomic DNA. Translation: BAB37185.1 .
    PIRi B91099.
    F85944.
    RefSeqi NP_289459.1. NC_002655.2.
    NP_311789.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali Q8XD57.
    SMRi Q8XD57. Positions 12-503.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z4228.

    Proteomic databases

    PRIDEi Q8XD57.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG58018 ; AAG58018 ; Z4228 .
    BAB37185 ; BAB37185 ; BAB37185 .
    GeneIDi 916412.
    958385.
    KEGGi ece:Z4228.
    ecs:ECs3762.
    PATRICi 18357007. VBIEscCol44059_3682.

    Phylogenomic databases

    eggNOGi COG1190.
    HOGENOMi HOG000236578.
    KOi K04567.
    OMAi GTEICLE.
    OrthoDBi EOG69PQ2M.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-3729-MONOMER.
    ECOO157:LYSS-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00252. Lys_tRNA_synth_class2.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR002313. Lys-tRNA-ligase_II.
    IPR018149. Lys-tRNA-synth_II_C.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    Pfami PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR00982. TRNASYNTHLYS.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00499. lysS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiSYK1_ECO57
    AccessioniPrimary (citable) accession number: Q8XD57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3