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Q8XD57

- SYK1_ECO57

UniProt

Q8XD57 - SYK1_ECO57

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Protein
Lysine--tRNA ligase
Gene
lysS, Z4228, ECs3762
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).UniRule annotation

Cofactori

Binds 3 magnesium ions per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi415 – 4151Magnesium 1 By similarity
Metal bindingi422 – 4221Magnesium 1 By similarity
Metal bindingi422 – 4221Magnesium 2 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. lysine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. nucleic acid binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. lysyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3729-MONOMER.
ECOO157:LYSS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:Z4228, ECs3762
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 505504Lysine--tRNA ligaseUniRule annotation
PRO_0000152624Add
BLAST

Proteomic databases

PRIDEiQ8XD57.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z4228.

Structurei

3D structure databases

ProteinModelPortaliQ8XD57.
SMRiQ8XD57. Positions 12-503.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1190.
HOGENOMiHOG000236578.
KOiK04567.
OMAiGTEICLE.
OrthoDBiEOG69PQ2M.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XD57-1 [UniParc]FASTAAdd to Basket

« Hide

MSEQHAQGAD AVVDLNNELK TRREKLANLR EQGIAFPNDF RRDHTSDQLH    50
AEFDGKENEE LEALNIEVAV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA 100
RDDLPEGVYN EQFKKWDLGD ILGAKGKLFK TKTGELSIHC TELRLLTKAL 150
RPLPDKFHGL QDQEARYRQR YLDLISNDES RNTFKVRSQI LSGIRQFMVN 200
RGFMEVETPM MQVIPGGAAA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG 250
GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYKDL IELTESLFRT 300
LAQDILGKTE VTYGDVTLDF GKPFEKLTMR EAIKKYRPET DMADLDNFDS 350
AKAIVESIGI HVEKSWGLGR IVTEIFEEVA EAHLIQPTFI TEYPAEVSPL 400
ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAQRF LDQVAAKDAG 450
DDEAMFYDED YVTALEHGLP PTAGLGIGID RMVMLFTNSH TIRDVILFPA 500
MRPVK 505
Length:505
Mass (Da):57,631
Last modified:January 23, 2007 - v3
Checksum:iCDABF71FAC12A629
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58018.1.
BA000007 Genomic DNA. Translation: BAB37185.1.
PIRiB91099.
F85944.
RefSeqiNP_289459.1. NC_002655.2.
NP_311789.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58018; AAG58018; Z4228.
BAB37185; BAB37185; BAB37185.
GeneIDi916412.
958385.
KEGGiece:Z4228.
ecs:ECs3762.
PATRICi18357007. VBIEscCol44059_3682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58018.1 .
BA000007 Genomic DNA. Translation: BAB37185.1 .
PIRi B91099.
F85944.
RefSeqi NP_289459.1. NC_002655.2.
NP_311789.1. NC_002695.1.

3D structure databases

ProteinModelPortali Q8XD57.
SMRi Q8XD57. Positions 12-503.
ModBasei Search...

Protein-protein interaction databases

STRINGi 155864.Z4228.

Proteomic databases

PRIDEi Q8XD57.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG58018 ; AAG58018 ; Z4228 .
BAB37185 ; BAB37185 ; BAB37185 .
GeneIDi 916412.
958385.
KEGGi ece:Z4228.
ecs:ECs3762.
PATRICi 18357007. VBIEscCol44059_3682.

Phylogenomic databases

eggNOGi COG1190.
HOGENOMi HOG000236578.
KOi K04567.
OMAi GTEICLE.
OrthoDBi EOG69PQ2M.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-3729-MONOMER.
ECOO157:LYSS-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
HAMAPi MF_00252. Lys_tRNA_synth_class2.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
Pfami PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR00982. TRNASYNTHLYS.
SUPFAMi SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00499. lysS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiSYK1_ECO57
AccessioniPrimary (citable) accession number: Q8XD57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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