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Q8XD23 (GLSA1_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase 1

EC=3.5.1.2
Gene names
Name:glsA1
Ordered Locus Names:Z0606, ECs0538
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00313

Sequence similarities

Belongs to the glutaminase family.

Ontologies

Keywords
   Molecular functionHydrolase
   PTMAcetylation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Glutaminase 1 HAMAP-Rule MF_00313
PRO_0000110609

Sites

Binding site661Substrate By similarity
Binding site1171Substrate By similarity
Binding site1611Substrate By similarity
Binding site1681Substrate By similarity
Binding site1921Substrate By similarity
Binding site2441Substrate By similarity
Binding site2621Substrate; via amide nitrogen By similarity

Amino acid modifications

Modified residue2941N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XD23 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 9B335D6AA51CB378

FASTA31032,844
        10         20         30         40         50         60 
MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTSDG NVYSAGDSDY 

        70         80         90        100        110        120 
RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA 

       130        140        150        160        170        180 
IATTSLINAE NAEQRWQRIL HIQQQLAGEL VALSDEVNQS EQTTNFHNRA IAWLLYSAGY 

       190        200        210        220        230        240 
LYCDAMEACD VYTRQCSTLI NTVELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM 

       250        260        270        280        290        300 
EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEEGNSV RGQKMVASVA 

       310 
KQLGYNVFKG 

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG54834.1.
BA000007 Genomic DNA. Translation: BAB33961.1.
PIRB90696.
F85546.
RefSeqNP_286226.1. NC_002655.2.
NP_308565.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8XD23.
SMRQ8XD23. Positions 2-310.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z0606.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG54834; AAG54834; Z0606.
BAB33961; BAB33961; BAB33961.
GeneID914642.
957462.
KEGGece:Z0606.
ecs:ECs0538.
PATRIC18350051. VBIEscCol44059_0546.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2066.
HOGENOMHOG000216890.
KOK01425.
OMAMGLHLMS.
OrthoDBEOG6N94BK.
ProtClustDBPRK12356.

Enzyme and pathway databases

BioCycECOL386585:GJFA-533-MONOMER.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLSA1_ECO57
AccessionPrimary (citable) accession number: Q8XD23
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2003
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families