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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Binding sitei197 – 1971ATPBy similarity
Binding sitei314 – 3141ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3434ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3764-MONOMER.
ECOO157:G7E-3-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:Z4265, ECs3797
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 387386Phosphoglycerate kinasePRO_0000145943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi155864.Z4265.

Structurei

3D structure databases

ProteinModelPortaliQ8XD03.
SMRiQ8XD03. Positions 2-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 233Substrate bindingBy similarity
Regioni59 – 624Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
KOiK00927.
OMAiDAFGTCH.
OrthoDBiEOG64N9Z0.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XD03-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVIKMTDLD LAGKRVFIRA DLNVPVKDGK VTSDARIRAS LPTIELALKQ
60 70 80 90 100
GAKVMVTSHL GRPTEGEYNE EFSLLPVVNY LKDKLSNPVR LVKDYLDGVD
110 120 130 140 150
VAEGELVVLE NVRFNKGEKK DDEILSKKYA ALCDVFVMDA FGTAHRAQAS
160 170 180 190 200
THGIGKFADV ACAGPLLAAE LDALGKALKE PARPMVAIVG GSKVSTKLTV
210 220 230 240 250
LDSLSKIADQ LIVGGGIANT FIAAQGHDVG KSLYEADLVD EAKRLLTTCN
260 270 280 290 300
IPVPSDVRVA TEFSETAPAT LKSVNDVKAD EQILDIGDAS AQELAEILKN
310 320 330 340 350
AKTILWNGPV GVFEFPNFRK GTEIVANAIA DSEAFSIAGG GDTLAAIDLF
360 370 380
GIADKISYIS TGGGAFLEFV EGKVLPAVAM LEERAKK
Length:387
Mass (Da):41,130
Last modified:January 23, 2007 - v3
Checksum:iC21AD662E33514B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58052.1.
BA000007 Genomic DNA. Translation: BAB37220.1.
PIRiE91103.
H85948.
RefSeqiNP_289493.1. NC_002655.2.
NP_311824.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58052; AAG58052; Z4265.
BAB37220; BAB37220; BAB37220.
GeneIDi916382.
957143.
KEGGiece:Z4265.
ecs:ECs3797.
PATRICi18357083. VBIEscCol44059_3720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58052.1.
BA000007 Genomic DNA. Translation: BAB37220.1.
PIRiE91103.
H85948.
RefSeqiNP_289493.1. NC_002655.2.
NP_311824.1. NC_002695.1.

3D structure databases

ProteinModelPortaliQ8XD03.
SMRiQ8XD03. Positions 2-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z4265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58052; AAG58052; Z4265.
BAB37220; BAB37220; BAB37220.
GeneIDi916382.
957143.
KEGGiece:Z4265.
ecs:ECs3797.
PATRICi18357083. VBIEscCol44059_3720.

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
KOiK00927.
OMAiDAFGTCH.
OrthoDBiEOG64N9Z0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciECOL386585:GJFA-3764-MONOMER.
ECOO157:G7E-3-MONOMER.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPGK_ECO57
AccessioniPrimary (citable) accession number: Q8XD03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.