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Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi45Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi67Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi99Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi103Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi106Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi112Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi154Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi157Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi201Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi228Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi231Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi235Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi263Iron-sulfur 4 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G
NUO7
Gene namesi
Name:nuoG
Ordered Locus Names:Z3542, ECs3167
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001185472 – 908NADH-quinone oxidoreductase subunit GAdd BLAST907

Keywords - PTMi

Quinone

Proteomic databases

PRIDEiQ8XCX2

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Protein-protein interaction databases

STRINGi155864.Z3542

Structurei

3D structure databases

ProteinModelPortaliQ8XCX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 832Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST82
Domaini83 – 1224Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini221 – 2774Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4108IEH Bacteria
COG1034 LUCA
HOGENOMiHOG000276177
KOiK00336
OMAiGYGYVNR

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XCX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIHVDGKE YEVNGADNLL EACLSLGLDI PYFCWHPALG SVGACRQCAV
60 70 80 90 100
KQYQNAEDTR GRLVMSCMTP ASDGTFISID DEEAKQFRES VVEWLMTNHP
110 120 130 140 150
HDCPVCEEGG NCHLQDMTVM TGHSFRRYRF TKRTHRNQDL GPFISHEMNR
160 170 180 190 200
CIACYRCVRY YKDYADGTDL GVYGAHDNVY FGRPEDGTLE SEFSGNLVEI
210 220 230 240 250
CPTGVFTDKT HSERYNRKWD MQFAPSICQQ CSIGCNISPG ERYGELRRIE
260 270 280 290 300
NRYNGTVNHY FLCDRGRFGY GYVNLKDRPR QPVQRRGDDF ITLNAEQAMQ
310 320 330 340 350
GAADILRQSK KVIGIGSPRA SVESNFALRE LVGEENFYTG IAHGEQERLQ
360 370 380 390 400
LALKVLREGG IYTPALREIE SYDAVLVLGE DVTQTGARVA LAVRQAVKGK
410 420 430 440 450
AREMAAAQKV ADWQIAAILN IGQRAKHPLF VTNVDDTRLD DIAAWTYRAP
460 470 480 490 500
VEDQARLGFA IAHALDNSAP AVDGIEPELQ SKIDVIVQAL AGAKKPLIIS
510 520 530 540 550
GTNAGSLEVI QAAANVAKAL KGRGADVGIT MIARSVNSMG LGIMGGGSLE
560 570 580 590 600
EALTELETGR ADAVVVLEND LHRHASATRV NAALAKAPLV MVVDHQRTAI
610 620 630 640 650
MENAHLVLSA ASFAESDGTV INNEGRAQRF FQVYDPAYYD SKTVMLESWR
660 670 680 690 700
WLHSLHSTLL SREVDWTQLD HVIDAVVAKI PELAGIKDAA PDATFRIRGQ
710 720 730 740 750
KLAREPHRYS GRTAMRANIS VHEPRQPQDI DTMFTFSMEG NNQPTAHRSQ
760 770 780 790 800
VPFAWAPGWN SPQAWNKFQD EVGGKLRFGD PGVRLFETSE NGLDYFTSVP
810 820 830 840 850
ARFQPQDGKW RIAPYYHLFG SDELSQRAPV FQCRMPQPYI KLNPADAAKL
860 870 880 890 900
GVNAGTRVSF SYDGNTVTLP VEIAEGLTAG QVGLPMGMSG IAPVLAGAHL

EDLKEAQQ
Length:908
Mass (Da):100,303
Last modified:January 23, 2007 - v3
Checksum:i048222FD1B9230CD
GO

Sequence cautioni

The sequence AAG57412 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB36590 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG57412.1 Different initiation.
BA000007 Genomic DNA Translation: BAB36590.1 Different initiation.
PIRiG91024
H85868
RefSeqiNP_311194.2, NC_002695.1
WP_001301902.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG57412; AAG57412; Z3542
BAB36590; BAB36590; BAB36590
GeneIDi916875
KEGGiece:Z3542
ecs:ECs3167
PATRICifig|386585.9.peg.3305

Similar proteinsi

Entry informationi

Entry nameiNUOG_ECO57
AccessioniPrimary (citable) accession number: Q8XCX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 107 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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