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Protein

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Gene

mnmC

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.UniRule annotation

Cofactori

FADUniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processtRNA processing
LigandFAD, Flavoprotein, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCUniRule annotation
Short name:
tRNA mnm(5)s(2)U biosynthesis bifunctional proteinUniRule annotation
Including the following 2 domains:
tRNA (mnm(5)s(2)U34)-methyltransferaseUniRule annotation (EC:2.1.1.61UniRule annotation)
FAD-dependent cmnm(5)s(2)U34 oxidoreductaseUniRule annotation (EC:1.5.-.-UniRule annotation)
Gene namesi
Name:mnmCUniRule annotation
Ordered Locus Names:Z3587, ECs3208
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950141 – 668tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCAdd BLAST668

Proteomic databases

PRIDEiQ8XCQ7

Interactioni

Protein-protein interaction databases

STRINGi155864.Z3587

Structurei

Secondary structure

1668
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni2 – 5Combined sources4
Beta strandi11 – 13Combined sources3
Beta strandi19 – 21Combined sources3
Turni22 – 25Combined sources4
Beta strandi26 – 29Combined sources4
Turni31 – 33Combined sources3
Helixi34 – 45Combined sources12
Helixi48 – 51Combined sources4
Helixi52 – 54Combined sources3
Beta strandi56 – 65Combined sources10
Helixi71 – 86Combined sources16
Beta strandi94 – 103Combined sources10
Helixi107 – 114Combined sources8
Helixi118 – 120Combined sources3
Helixi121 – 129Combined sources9
Beta strandi136 – 143Combined sources8
Beta strandi148 – 155Combined sources8
Helixi157 – 160Combined sources4
Helixi161 – 163Combined sources3
Helixi166 – 168Combined sources3
Beta strandi172 – 177Combined sources6
Turni182 – 184Combined sources3
Helixi186 – 188Combined sources3
Helixi191 – 200Combined sources10
Beta strandi201 – 210Combined sources10
Helixi214 – 223Combined sources10
Beta strandi225 – 230Combined sources6
Beta strandi239 – 244Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QY6X-ray2.00A/B2-247[»]
ProteinModelPortaliQ8XCQ7
SMRiQ8XCQ7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8XCQ7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 245tRNA (mnm(5)s(2)U34)-methyltransferaseAdd BLAST245
Regioni270 – 668FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd BLAST399

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the DAO family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CWG Bacteria
COG0665 LUCA
COG4121 LUCA
HOGENOMiHOG000218142
KOiK15461
OMAiTGLNFLC

Family and domain databases

Gene3Di3.50.50.60, 3 hits
HAMAPiMF_01102 MnmC, 1 hit
InterProiView protein in InterPro
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR008471 MnmC-like_methylTransf
IPR029063 SAM-dependent_MTases
IPR023032 tRNA_MAMT_biosynth_bifunc_MnmC
IPR017610 tRNA_S-uridine_synth_MnmC_C
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PF05430 Methyltransf_30, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR03197 MnmC_Cterm, 1 hit

Sequencei

Sequence statusi: Complete.

Q8XCQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHYSIQPAN LEFNAEGTPV SRDFDDVYFS NDNGLEETRY VFLGGNQLEA
60 70 80 90 100
RFPEHPHPLF VVAESGFGTG LNFLTLWQAF DQFREAHPQA QLQRLHFISF
110 120 130 140 150
EKFPLTRADL ALAHQHWPEL APWAEQLQAQ WPMPLPGCHR LLLDEGRVTL
160 170 180 190 200
DLWFGDINEL ISQLDDSLNQ KVDAWFLDGF APAKNPDMWT QNLFNAMARL
210 220 230 240 250
ARPGGTLATF TSAGFVRRGL QEAGFTMQKR KGFGRKREML CGVMEQTLPL
260 270 280 290 300
PCSTPWFNRT GSSKREVAII GGGIASALLS LALLRRGWQV TLYCADEAPA
310 320 330 340 350
LGASGNRQGA LYPLLSKHDE ALNRFFSNGF TFARRLYDSL PVKFDHDWCG
360 370 380 390 400
VTQLGWDEKS QHKIAQMLSM DLPEELAVAV EANAVEQITG VTTNCSGITY
410 420 430 440 450
PQGGWLCPAE LTRNVLELAQ QQGLQIYYQY QLQDLSRKDD CWLLTFAGDQ
460 470 480 490 500
QATHSVVVLA NGHQISRFSQ TSSLPVYSVA GQVSHIPTTP ELAKLKQVLC
510 520 530 540 550
YDGYLTPQNP ANQHHCIGAS YHRGSEETAY SEDNQQQNRQ RLIDCFPHAQ
560 570 580 590 600
WAKTVDVSKK EARCGVRCAT RDHLPMVGNV PDYEATLVEY ASLAEQKDKA
610 620 630 640 650
VSAPVFDDLF MFAALGSRGL CSAPLCAEIL AAQMSDEPIP MDASTLAALN
660
PNRLWVRKLL KGKAVKAG
Length:668
Mass (Da):74,468
Last modified:August 30, 2002 - v2
Checksum:i698D0E08AD0908BF
GO

Sequence cautioni

The sequence AAG57453 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB36631 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG57453.1 Different initiation.
BA000007 Genomic DNA Translation: BAB36631.1 Different initiation.
PIRiA85874
H91029
RefSeqiNP_311235.2, NC_002695.1
WP_000683769.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG57453; AAG57453; Z3587
BAB36631; BAB36631; BAB36631
GeneIDi913957
KEGGiece:Z3587
ecs:ECs3208
PATRICifig|386585.9.peg.3349

Similar proteinsi

Entry informationi

Entry nameiMNMC_ECO57
AccessioniPrimary (citable) accession number: Q8XCQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: August 30, 2002
Last modified: May 23, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health