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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.UniRule annotation

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.UniRule annotation

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (zwf)
  2. 6-phosphogluconolactonase (pgl)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (gnd)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50NADPUniRule annotation1
Binding sitei147NADPUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Binding sitei181SubstrateUniRule annotation1
Binding sitei215SubstrateUniRule annotation1
Binding sitei234SubstrateUniRule annotation1
Active sitei239Proton acceptorUniRule annotation1
Binding sitei339SubstrateUniRule annotation1
Binding sitei344SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 93NADPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciECOO157:ZWF-MONOMER.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
Short name:
G6PDUniRule annotation
Gene namesi
Name:zwfUniRule annotation
Ordered Locus Names:Z2904, ECs2562
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000681191 – 491Glucose-6-phosphate 1-dehydrogenaseAdd BLAST491

Interactioni

Protein-protein interaction databases

STRINGi155864.Z2904.

Structurei

3D structure databases

ProteinModelPortaliQ8XCJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8W. Bacteria.
COG0364. LUCA.
HOGENOMiHOG000046191.
KOiK00036.
OMAiGGICANL.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XCJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTQTAQVC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR
60 70 80 90 100
ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS
110 120 130 140 150
RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG
160 170 180 190 200
TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN
210 220 230 240 250
WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS
260 270 280 290 300
PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG
310 320 330 340 350
YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV
360 370 380 390 400
YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ
410 420 430 440 450
ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD
460 470 480 490
SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Length:491
Mass (Da):55,732
Last modified:March 1, 2002 - v1
Checksum:iFEE38F456F6A7B24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56842.1.
BA000007 Genomic DNA. Translation: BAB35985.1.
PIRiB90949.
F85797.
RefSeqiNP_310589.1. NC_002695.1.
WP_000301737.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG56842; AAG56842; Z2904.
BAB35985; BAB35985; BAB35985.
GeneIDi912956.
KEGGiece:Z2904.
ecs:ECs2562.
PATRICi18354494. VBIEscCol44059_2458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56842.1.
BA000007 Genomic DNA. Translation: BAB35985.1.
PIRiB90949.
F85797.
RefSeqiNP_310589.1. NC_002695.1.
WP_000301737.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliQ8XCJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z2904.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56842; AAG56842; Z2904.
BAB35985; BAB35985; BAB35985.
GeneIDi912956.
KEGGiece:Z2904.
ecs:ECs2562.
PATRICi18354494. VBIEscCol44059_2458.

Phylogenomic databases

eggNOGiENOG4105D8W. Bacteria.
COG0364. LUCA.
HOGENOMiHOG000046191.
KOiK00036.
OMAiGGICANL.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
BioCyciECOO157:ZWF-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD_ECO57
AccessioniPrimary (citable) accession number: Q8XCJ6
Secondary accession number(s): Q7AD71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.