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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.By similarity

Catalytic activityi

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10D-glucose 6-phosphateBy similarity1
Binding sitei77D-glucose 6-phosphateBy similarity1
Sitei86Involved in alpha anomer selectivityBy similarity1
Binding sitei131D-glucose 6-phosphateBy similarity1
Sitei156Involved in alpha anomer selectivityBy similarity1
Binding sitei263UDP-glucoseBy similarity1
Binding sitei268UDP-glucoseBy similarity1
Binding sitei301D-glucose 6-phosphateBy similarity1
Binding sitei340UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299

Protein family/group databases

CAZyiGT20 Glycosyltransferase Family 20

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
UDP-glucose-glucosephosphate glucosyltransferaseBy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:Z2949, ECs2604
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001224902 – 474Trehalose-6-phosphate synthaseAdd BLAST473

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi155864.Z2949

Structurei

3D structure databases

ProteinModelPortaliQ8XCE7
SMRiQ8XCE7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 23UDP-glucose bindingBy similarity2
Regioni366 – 370UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

eggNOGiENOG4105C1K Bacteria
COG0380 LUCA
HOGENOMiHOG000191478
KOiK00697
OMAiIEFMPIH

Family and domain databases

CDDicd03788 GT1_TPS, 1 hit
InterProiView protein in InterPro
IPR001830 Glyco_trans_20
IPR012766 Trehalose_OtsA
PfamiView protein in Pfam
PF00982 Glyco_transf_20, 1 hit
TIGRFAMsiTIGR02400 trehalose_OtsA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XCE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED
60 70 80 90 100
QPLKKVKKGN ITWASFNLSE QDLDEYYNQF SNAVLWPAFH YRLDLVQFQR
110 120 130 140 150
PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN
210 220 230 240 250
LTRVTTRSAK SHTAWGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA
260 270 280 290 300
ELKNVQNIFS VERLDYSKGL PERFLAYEAL LEKYPQHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI
410 420 430 440 450
VNPYDRDEVA VALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD
460 470
LKQIVPRSAE SQQRDKVATF PKLV
Length:474
Mass (Da):53,667
Last modified:January 23, 2007 - v3
Checksum:i63FFA9F628056F82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG56885.1
BA000007 Genomic DNA Translation: BAB36027.1
PIRiA85803
D90954
RefSeqiNP_310631.1, NC_002695.1
WP_000089030.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG56885; AAG56885; Z2949
BAB36027; BAB36027; BAB36027
GeneIDi912524
KEGGiece:Z2949
ecs:ECs2604
PATRICifig|386585.9.peg.2730

Similar proteinsi

Entry informationi

Entry nameiOTSA_ECO57
AccessioniPrimary (citable) accession number: Q8XCE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 86 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health