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Q8XBT6 (DKGA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,5-diketo-D-gluconic acid reductase A
Short name=2,5-DKG reductase A
Short name=2,5-DKGR A
Short name=25DKGR-A
EC=1.1.1.274
Alternative name(s):
AKR5C
Gene names
Name:dkgA
Ordered Locus Names:Z4365, ECs3896
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.

Catalytic activity

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Sequence similarities

Belongs to the aldo/keto reductase family.

Sequence caution

The sequence AAG58148.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB37319.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAscorbate biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processL-ascorbic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function2,5-didehydrogluconate reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2752752,5-diketo-D-gluconic acid reductase A
PRO_0000124600

Regions

Nucleotide binding187 – 24155NADP By similarity

Sites

Active site511Proton donor By similarity
Binding site1071Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XBT6 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: A807C84DB1B7F78B

FASTA27531,130
        10         20         30         40         50         60 
MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSFDTAAA YKNEEGVGKA 

        70         80         90        100        110        120 
LKNASVNREE LFITTKLWND DHNRPREALL DSLKKLQLDY IDLYLMHWPV PAIDHYVEAW 

       130        140        150        160        170        180 
KGMIELQKEG LIKSIGVCNF QIHHLQRLID ETGVTPVINQ IELHPLMQQR QLHAWNATHK 

       190        200        210        220        230        240 
IQTESWSPLA QGGKGVFDQK VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE 

       250        260        270 
NFDVWDFRLD KDELGEIAKL DQGKRLGPDP DQFGG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG58148.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB37319.1. Different initiation.
PIRH85960.
H91115.
RefSeqNP_289589.2. NC_002655.2.
NP_311923.2. NC_002695.1.

3D structure databases

ProteinModelPortalQ8XBT6.
SMRQ8XBT6. Positions 1-275.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000028353; EBESCP00000027246; EBESCG00000027404.
EBESCT00000059121; EBESCP00000056949; EBESCG00000058169.
GeneID916279.
957304.
GenomeReviewsGene locus Z4365 in contig AE005174_GR.
Gene locus ECs3896 in contig BA000007_GR.
KEGGece:Z4365.
ecs:ECs3896.
PATRIC18357283. VBIEscCol44059_3819.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009262.
HOGENOMHBG605727.
OMAWNGDLGY.
ProtClustDBPRK11565.

Enzyme and pathway databases

BioCycECOL83334:ECS3896-MONOMER.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
KOK06221.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. Aldo/ket_red. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDKGA_ECO57
AccessionPrimary (citable) accession number: Q8XBT6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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