Q8XBK7 (TTDA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L(+)-tartrate dehydratase subunit alpha
Short name=L-TTD alpha
EC=4.2.1.32

Gene names

Name:	ttdA
Ordered Locus Names:	Z4414, ECs3944

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(R,R)-tartrate = oxaloacetate + H2O.
Cofactor	Iron-sulfur By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Induction	Induced by tartrate, via ttdR By similarity.
Sequence similarities	Belongs to the class-I fumarase family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW L(+)-tartrate dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	L(+)-tartrate dehydratase subunit alpha		PRO_0000262696
Sites
	Metal binding	71	1	Iron-sulfur By similarity
	Metal binding	190	1	Iron-sulfur By similarity
	Metal binding	277	1	Iron-sulfur By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XBK7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4BA9141EA22240AC	FASTA	303	32,677
	10 20 30 40 50 60 MMSESNKQQA VNKLTEIVAN FTAMISTRMP DDVVDKLKQL KDAETSSMGK IIYHTMFDNM 70 80 90 100 110 120 QKAIDLNRPA CQDTGEIMFF VKVGSRFPLL GELQSILKQA VEEATIKAPL RHNAVEIFDE 130 140 150 160 170 180 VNTGKNTGSG VPWVTWDIVP DGDDAEIEVY MAGGGCTLPG RSKVLMPSEG YEGVVKFVFE 190 200 210 220 230 240 NISTLAVNAC PPVLVGVGIA TSVETAAVLS RKAILRPIGS RHPNPKAAEL ELRLEEGLNR 250 260 270 280 290 300 LGIGPQGLTG NSSVMGVHIE SAARHPSTIG VAVSTGCWAH RRGTLLVHAD LTFENLSHTR SAL

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58195.1. BA000007 Genomic DNA. Translation: BAB37367.1.
PIR	G85966. H91121.
RefSeq	NP_289636.1. NC_002655.2. NP_311971.1. NC_002695.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000028526; EBESCP00000027419; EBESCG00000027577. EBESCT00000059282; EBESCP00000057110; EBESCG00000058330.
GeneID	916192. 958530.
GenomeReviews	Gene locus Z4414 in contig AE005174_GR. Gene locus ECs3944 in contig BA000007_GR.
KEGG	ece:Z4414. ecs:ECs3944.
PATRIC	18357383. VBIEscCol44059_3869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010386.
HOGENOM	HBG548096.
OMA	ESSARHP.
ProtClustDB	PRK08230.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3944-MONOMER.
Family and domain databases
InterPro	IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat. [Graphical view]
KO	K03779.
Pfam	PF05681. Fumerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00722. TtdA_fumA_fumB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TTDA_ECO57

Accession

Primary (citable) accession number: Q8XBK7
Secondary accession number(s): Q7AAQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program