Q8XB35 (TYPH_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine phosphorylase
EC=2.4.2.4

Alternative name(s):
TdRPase

Gene names

Name:	deoA
Ordered Locus Names:	Z5984, ECs5341

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis By similarity. HAMAP-Rule MF_01628
Catalytic activity	Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01628
Pathway	Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. HAMAP-Rule MF_01628
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01628
Sequence similarities	Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyrimidine nucleobase metabolic process Inferred from electronic annotation. Source: InterPro thymidine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	phosphorylase activity Inferred from electronic annotation. Source: InterPro pyrimidine-nucleoside phosphorylase activity Inferred from electronic annotation. Source: InterPro thymidine phosphorylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Thymidine phosphorylase HAMAP-Rule MF_01628		PRO_0000059055

Sequences

	Sequence		Length	Mass (Da)
	Q8XB35 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: B3BDF83C9275D2F1	FASTA	440	47,180
	10 20 30 40 50 60 MFLAQEIIRK KRDGHALSDE EIRFFINGIR DNTISEGQIA ALAMTIFFHD MTMPERVSLT 70 80 90 100 110 120 MAMRDSGTVL DWKSLHLNGP IVDKHSTGGV GDVTSLMLGP MVAACGGYIP MISGRGLGHT 130 140 150 160 170 180 GGTLDKLESI PGFDIFPDDN RFREIIKDVG VAIIGQTSSL APADKRFYAT RDITATVDSI 190 200 210 220 230 240 PLITASILAK KLAEGLDALV MDVKVGSGAF MPTYELSEAL AEAIVGVANG AGVRTTALLT 250 260 270 280 290 300 DMNQVLASSA GNAVEVREAV QFLTGEYRNP RLFDVTMALC VEMLISGKLA KDDAEARAKL 310 320 330 340 350 360 QAVLDNGKAA EVFGRMVAAQ KGPTDFVENY AKYLPTAMLT KAVYADTEGF VSEMDTRALG 370 380 390 400 410 420 MAVVAMGGGR RQASDTIDYS VGFTDMARLG DQVDGQRPLA VIHAKDENSW QEAAKAVKAA 430 440 IKLADKAPES TPTVYRRISE

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG59563.1. BA000007 Genomic DNA. Translation: BAB38764.1.
PIR	E91296. G86137.
RefSeq	NP_290996.1. NC_002655.2. NP_313368.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8XB35.
SMR	Q8XB35. Positions 1-440.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z5984.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG59563; AAG59563; Z5984. BAB38764; BAB38764; BAB38764.
GeneID	913506. 959672.
KEGG	ece:Z5984. ecs:ECs5341.
PATRIC	18360340. VBIEscCol44059_5300.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0213.
HOGENOM	HOG000047313.
KO	K00758.
OMA	LMAIYFR.
ProtClustDB	PRK05820.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-5353-MONOMER.
UniPathway	UPA00578; UER00638.
Family and domain databases
Gene3D	3.40.1030.10. 1 hit. 3.90.1170.30. 1 hit.
HAMAP	MF_01628. Thymid_phosp.
InterPro	IPR000312. Glycosyl_Trfase_fam3. IPR017459. Glycosyl_Trfase_fam3_N_dom. IPR013102. PYNP_C. IPR018090. Pyrmidine_PPas_bac/euk. IPR000053. Pyrmidine_PPase. IPR017872. Pyrmidine_PPase_CS. IPR013465. Thymidine_Pase. [Graphical view]
PANTHER	PTHR10515. PTHR10515. 1 hit.
Pfam	PF02885. Glycos_trans_3N. 1 hit. PF00591. Glycos_transf_3. 1 hit. PF07831. PYNP_C. 1 hit. [Graphical view]
PIRSF	PIRSF000478. TP_PyNP. 1 hit.
SMART	SM00941. PYNP_C. 1 hit. [Graphical view]
SUPFAM	SSF47648. Glyco_trans_3. 1 hit. SSF52418. Glyco_trans_3. 1 hit. SSF54680. PYNP_C. 1 hit.
TIGRFAMs	TIGR02643. T_phosphoryl. 1 hit. TIGR02644. Y_phosphoryl. 1 hit.
PROSITE	PS00647. THYMID_PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYPH_ECO57

Accession

Primary (citable) accession number: Q8XB35
Secondary accession number(s): Q8X2H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program