Q8XAT5 (GPPA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
EC=3.6.1.40

Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase

Gene names

Name:	gppA
Ordered Locus Names:	Z5289, ECs4712

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities By similarity. HAMAP-Rule MF_01550
Catalytic activity	Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate. HAMAP-Rule MF_01550
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP-Rule MF_01550
Sequence similarities	Belongs to the GppA/Ppx family. GppA subfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	guanosine pentaphosphate catabolic process Inferred from electronic annotation. Source: InterPro guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP-Rule MF_01550		PRO_0000194281

Sequences

	Sequence		Length	Mass (Da)
	Q8XAT5 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C4DD8DC59432A5F9	FASTA	494	54,871
	10 20 30 40 50 60 MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN ALSNEAMERG 70 80 90 100 110 120 WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA QEILGCPVQV ISGEEEARLI 130 140 150 160 170 180 YQGVAHTTGG ADQRLVVDIG GASTELVTGT GAQTTSLFSL SMGCVTWLER YFADRNLGQE 190 200 210 220 230 240 NFDAAEKAAR EVLRPVADEL RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK 250 260 270 280 290 300 QRAIHCGRLE ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML 310 320 330 340 350 360 HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI SRDLLISACQ 370 380 390 400 410 420 LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL LLNQTNPVDL SSLHQQNAVP 430 440 450 460 470 480 PRVAEQLCRL LRLAIIFASR RRDDLVPEMT LQANHELLTL TLPQGWLTQH PLGKEIIAQE 490 SQWQSYVHWP LEVH

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58973.1. BA000007 Genomic DNA. Translation: BAB38135.1.
PIR	A86064. H91217.
RefSeq	NP_290409.1. NC_002655.2. NP_312739.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8XAT5.
SMR	Q8XAT5. Positions 8-493.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z5289.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG58973; AAG58973; Z5289. BAB38135; BAB38135; BAB38135.
GeneID	915259. 960391.
KEGG	ece:Z5289. ecs:ECs4712.
PATRIC	18359031. VBIEscCol44059_4674.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0248.
HOGENOM	HOG000258672.
KO	K01524.
OMA	PPRVAEH.
ProtClustDB	PRK11031.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-4686-MONOMER.
UniPathway	UPA00908; UER00884.
Family and domain databases
HAMAP	MF_01550. GppA.
InterPro	IPR023709. Guo-5TP_3DP_PyrP. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPPA_ECO57

Accession

Primary (citable) accession number: Q8XAT5
Secondary accession number(s): Q7A9F8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program