Reviewed, UniProtKB/Swiss-Prot Q8XAQ8 (WECF_ECO57)

Names and origin

Protein names

Recommended name:
    4-alpha-L-fucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    TDP-Fuc4NAc:lipid II Fuc4NAc transferase
      Short name=Fuc4NAc transferase

Gene names

Name:	wecF
Synonyms:	rffT
Ordered Locus Names:	Z5304, ECs4726

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis By similarity.
Catalytic activity	TDP-Fuc4NAc + Und-PP-GlcNAc-ManNAcA = TDP + Und-PP-GlcNAc-ManNAcA-Fuc4NAc. HAMAP MF_01002
Pathway	Bacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis. HAMAP MF_01002
Subcellular location	Cell inner membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 56 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	enterobacterial common antigen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	4-alpha-L-fucosyltransferase HAMAP MF_01002		PRO_0000216182

Sequences

	Sequence		Length	Mass (Da)
	Q8XAQ8-1 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 0510A64D395466D9	FASTA	359	40,541
	10 20 30 40 50 60 MTVLIHVLGS DIPHHNRTVL RFFNDALAAT SEHAREFMVV GKDDGLSDSC PALSVQFFPG 70 80 90 100 110 120 KKSLAEAVIA KAKANRQQRF FFHGQFNPTL WLALLSGGIK PSQFYWHIWG ADLYELSSGL 130 140 150 160 170 180 RYKLFYPLRR LAQKRVGCVF ATRGDLSFFA KTHPKVRGEL LYFPTRMDAS LNTMANDRQR 190 200 210 220 230 240 EGKMTILVGN SGDRSNEHIA ALRAVHQQFG DTVKVVVPMG YPPHNEAYIE EVRQAGLELF 250 260 270 280 290 300 SEENLQVLSE KLEFDAYLTL LRQCALGYFI FARQQGIGTL CLLIQAGIPC VLNRENPFWQ 310 320 330 340 350 DMTEQHLPVL FTTDDLNEDI VREAQRQLAS VDKNTIAFFS PNYLQGWQRA LAIAAGEVA

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG58988.1. BA000007 Genomic DNA. Translation: BAB38149.1.
PIR	F91219. H86065.
RefSeq	NP_290424.1. NP_312753.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT56. Glycosyltransferase Family 56.
Genome annotation databases
GeneID	915126. 960373.
GenomeReviews	Gene locus Z5304 in contig AE005174_GR. Gene locus ECs4726 in contig BA000007_GR.
KEGG	ece:Z5304. ecs:ECs4726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XAQ8.
OMA	Q8XAQ8. IPHHNQT.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4726-MON.
Family and domain databases
HAMAP	MF_01002. [Tree]
InterPro	IPR009993. 4-alpha-FUT. [Graphical view]
Pfam	PF07429. Fuc4NAc_transf. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WECF_ECO57

Accession

Primary (citable) accession number: Q8XAQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)