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Protein

Urease subunit alpha

Gene

ureC1

more
Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB1), Urease subunit gamma (ureA1), Urease subunit alpha (ureC1)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi135Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi137Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi218Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi218Nickel 2; via carbamate groupUniRule annotation1
Binding sitei220SubstrateUniRule annotation1
Metal bindingi247Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi273Nickel 2; via tele nitrogenUniRule annotation1
Active sitei321Proton donorUniRule annotation1
Metal bindingi361Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureC1UniRule annotation
Ordered Locus Names:Z1145, ECs1324
AND
Name:ureC2UniRule annotation
Ordered Locus Names:Z1584
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002341551 – 568Urease subunit alphaAdd BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PRIDEiQ8XAG0.

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z1584.

Structurei

3D structure databases

ProteinModelPortaliQ8XAG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini130 – 568UreaseUniRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XAG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSNISRQAY ADMFGPTTGD KIRLADTELW IEVEDDLTTY GEEVKFGGGK
60 70 80 90 100
VIRDGMGQGQ MLSAGCADLV LTNALIIDYW GIVKADIGVK DGRIFAIGKA
110 120 130 140 150
GNPDIQPNVT IPIGVSTEII AAEGRIVTAG GVDTHIHWIC PQQAEEALTS
160 170 180 190 200
GITTMIGGGT GPTAGSNATT CTPGPWYIYQ MLQAADSLPV NIGLLGKGNC
210 220 230 240 250
SNPDALREQV AAGVIGLKIH EDWGATPAVI NCALTVADEM DVQVALHSDT
260 270 280 290 300
LNESGFVEDT LTAIGGRTIH TFHTEGAGGG HAPDIITACA HPNILPSSTN
310 320 330 340 350
PTLPYTVNTI DEHLDMLMVC HHLDPDIAED VAFAESRIRQ ETIAAEDVLH
360 370 380 390 400
DLGAFSLTSS DSQAMGRVGE VVLRTWQVAH RMKVQRGPLP EESGDNDNVR
410 420 430 440 450
VKRYIAKYTI NPALTHGIAH EVGSIEVGKL ADLVLWSPAF FGVKPATIVK
460 470 480 490 500
GGMIAMAPMG DINGSIPTPQ PVHYRPMFAA LGSARHRCRV TFLSQAAAAN
510 520 530 540 550
GVAEQLNLHS TTAVVKGCRT VQKADMRHNS LLPDITVDSQ TYEVRINGEL
560
ITSEPADILP MAQRYFLF
Length:568
Mass (Da):60,626
Last modified:March 1, 2002 - v1
Checksum:iAC39B7B701481361
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG55290.1.
AE005174 Genomic DNA. Translation: AAG55699.1.
BA000007 Genomic DNA. Translation: BAB34747.1.
PIRiD90794.
G85654.
RefSeqiNP_309351.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG55290; AAG55290; Z1145.
AAG55699; AAG55699; Z1584.
BAB34747; BAB34747; BAB34747.
GeneIDi913507.
KEGGiece:Z1145.
ece:Z1584.
ecs:ECs1324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG55290.1.
AE005174 Genomic DNA. Translation: AAG55699.1.
BA000007 Genomic DNA. Translation: BAB34747.1.
PIRiD90794.
G85654.
RefSeqiNP_309351.1. NC_002695.1.

3D structure databases

ProteinModelPortaliQ8XAG0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z1584.

Protein family/group databases

MEROPSiM38.982.

Proteomic databases

PRIDEiQ8XAG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG55290; AAG55290; Z1145.
AAG55699; AAG55699; Z1584.
BAB34747; BAB34747; BAB34747.
GeneIDi913507.
KEGGiece:Z1145.
ece:Z1584.
ecs:ECs1324.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_ECO57
AccessioniPrimary (citable) accession number: Q8XAG0
Secondary accession number(s): Q7AFH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Neither O157 strain expresses urease due to a truncation of ureD, the last gene of the probable operon. Urease activity is restored in O157 / Sakai upon complementation with wild-type ureD.Curated
This region of the chromosome is duplicated in strain O157:H7 / EDL933 but not in O157:H7 / Sakai.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.