Reviewed, UniProtKB/Swiss-Prot Q8XA73 (HCAF_ECO57)

Names and origin

Protein names

Recommended name:
    3-phenylpropionate/cinnamic acid dioxygenase subunit beta
    EC=1.14.12.19
Alternative name(s):
    Digoxigenin subunit beta

Gene names

Name:	hcaF
Ordered Locus Names:	Z3810, ECs3405

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity.
Catalytic activity	3-phenylpropanoic acid + NADH + O(2) = cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD(+). Cinnamic acid + H(+) + NADH + O(2) = cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-phenylpropionate dioxygenase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 172	172	3-phenylpropionate/cinnamic acid dioxygenase subunit beta		PRO_0000333711

Sequences

	Sequence		Length	Mass (Da)
	Q8XA73-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 83962E2D02415546	FASTA	172	20,526
	10 20 30 40 50 60 MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI CYTMRTTVNA QTRDRRKGVQ 70 80 90 100 110 120 PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQISETDIP NVFAVRVNYL 130 140 150 160 170 LYRAQKERDE TFYVGTRFDK VRRLEDDNWR LLERDIVLDQ AVITSHNLSV LF

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG57652.1. BA000007 Genomic DNA. Translation: BAB36828.1.
PIR	E91054. H85898.
RefSeq	NP_289095.1. NP_311432.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	914927. 957854.
GenomeReviews	Gene locus ECs3405 in contig BA000007_GR. Gene locus Z3810 in contig AE005174_GR.
KEGG	ece:Z3810. ecs:ECs3405.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XA73.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3405-MON.
Family and domain databases
HAMAP	MF_01649. [Tree]
InterPro	IPR000391. Rng_hydr_dOase-B. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCAF_ECO57

Accession

Primary (citable) accession number: Q8XA73
Secondary accession number(s): Q7ABL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 1, 2002
Last modified:	November 4, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)