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Protein

3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase

Gene

hcaB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively.UniRule annotation

Catalytic activityi

3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+ = 3-(2,3-dihydroxyphenyl)propanoate + NADH.UniRule annotation
(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+ = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NADH.UniRule annotation

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431SubstrateUniRule annotation
Active sitei156 – 1561Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3425NADUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3370-MONOMER.
ECOO157:HCAB-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenaseUniRule annotation (EC:1.3.1.87UniRule annotation)
Alternative name(s):
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenaseUniRule annotation
3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenaseUniRule annotation
CI-dihydrodiol dehydrogenaseUniRule annotation
Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenaseUniRule annotation
Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenaseUniRule annotation
PP-dihydrodiol dehydrogenaseUniRule annotation
Gene namesi
Name:hcaBUniRule annotation
Ordered Locus Names:Z3813, ECs3407
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2702703-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenasePRO_0000333761Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z3813.

Structurei

3D structure databases

ProteinModelPortaliQ8XA72.
SMRiQ8XA72. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the short-chain dehydrogenases/reductases (SDR) family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108QJ2. Bacteria.
ENOG410XQCB. LUCA.
KOiK05711.
OMAiEVFHINV.
OrthoDBiEOG625JVT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01647. HcaB.
InterProiIPR023643. Dihydrodiol_DH_HcaB.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XA72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF
60 70 80 90 100
GEHILAVEGN VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT
110 120 130 140 150
PAETLETGFH ELFNVNVLGY LLGAKACAPA LIAGEGSMIF TLSNAAWYPG
160 170 180 190 200
GGGPLYTASK HAATGLIRQL AYELAPKVRV NGVGPCGMAS DLRGPQALGQ
210 220 230 240 250
SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLASRR NNRALSGVMI
260 270
NADAGLAIRG IRHVAAGLDL
Length:270
Mass (Da):28,440
Last modified:March 1, 2002 - v1
Checksum:i6BFE56F10442C2A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57654.1.
BA000007 Genomic DNA. Translation: BAB36830.1.
PIRiB85899.
G91054.
RefSeqiNP_311434.1. NC_002695.1.
WP_001281372.1. NZ_LPWC01000059.1.

Genome annotation databases

EnsemblBacteriaiAAG57654; AAG57654; Z3813.
BAB36830; BAB36830; BAB36830.
GeneIDi914919.
KEGGiece:Z3813.
ecs:ECs3407.
PATRICi18356246. VBIEscCol44059_3318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57654.1.
BA000007 Genomic DNA. Translation: BAB36830.1.
PIRiB85899.
G91054.
RefSeqiNP_311434.1. NC_002695.1.
WP_001281372.1. NZ_LPWC01000059.1.

3D structure databases

ProteinModelPortaliQ8XA72.
SMRiQ8XA72. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z3813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57654; AAG57654; Z3813.
BAB36830; BAB36830; BAB36830.
GeneIDi914919.
KEGGiece:Z3813.
ecs:ECs3407.
PATRICi18356246. VBIEscCol44059_3318.

Phylogenomic databases

eggNOGiENOG4108QJ2. Bacteria.
ENOG410XQCB. LUCA.
KOiK05711.
OMAiEVFHINV.
OrthoDBiEOG625JVT.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciECOL386585:GJFA-3370-MONOMER.
ECOO157:HCAB-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01647. HcaB.
InterProiIPR023643. Dihydrodiol_DH_HcaB.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiHCAB_ECO57
AccessioniPrimary (citable) accession number: Q8XA72
Secondary accession number(s): Q7ABL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2002
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.