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Q8XA71 (HCAD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component
EC=1.18.1.3
Alternative name(s):
Digoxigenin system ferredoxin--NAD(+) reductase component
Gene names
Name:hcaD
Ordered Locus Names:Z3814, ECs3408
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP MF_01651

Catalytic activity

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH. HAMAP MF_01651

Cofactor

FAD By similarity. HAMAP MF_01651

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01651

Subunit structure

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity.

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4004003-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component HAMAP MF_01651
PRO_0000333725

Regions

Nucleotide binding5 – 3632FAD Potential
Nucleotide binding146 – 17429NAD Potential

Sequences

Sequence LengthMass (Da)Tools
Q8XA71 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: F884158DF79CBC2B

FASTA40043,906
        10         20         30         40         50         60 
MKEKTIIIVG GGQAAAMAAA SLRQQGFTGE LHLFSDEQHL PYERPPLSKS MLLEDSPQLQ 

        70         80         90        100        110        120 
SVLPAHWWQE NNVHLHSGVT IKTLGRDTRE LVLANGESWH WDQLFIATGA AARPLPLLDA 

       130        140        150        160        170        180 
LGERCFTLRH AGDAARLREV LQPERSVVIV GAGTIGLELA ASATQRRCKV TVIELAATVM 

       190        200        210        220        230        240 
GRNAPPPVQR YLLQRHQQAG VRILLNNAIE HVVDGEKVEL TLQSGETLQA DVVIYGIGIS 

       250        260        270        280        290        300 
ANDQLAREAN LDTTNGIVID EACRTCDPAI FAGGDVAITR LDNGALHRCE SWENANNHAQ 

       310        320        330        340        350        360 
IAAAAMLGLP LPLLPPPWFW SDQYSDNLQF IGDMRGDDWL CRGNPETQKA IWFNLQNGVL 

       370        380        390        400 
IGAVTLNQGR EIRSIRKWIQ SGKTFDAKQL TDENIALKSL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG57655.1.
BA000007 Genomic DNA. Translation: BAB36831.1.
PIRC85899.
H91054.
RefSeqNP_289098.1. NC_002655.2.
NP_311435.1. NC_002695.1.

3D structure databases

HSSPHSSP built from PDB template 1M6I based on UniProtKB O95831.
ProteinModelPortalQ8XA71.
SMRQ8XA71. Positions 3-400.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000028714; EBESCP00000027607; EBESCG00000027765.
EBESCT00000058841; EBESCP00000056669; EBESCG00000057889.
GeneID914925.
958539.
GenomeReviewsGene locus Z3814 in contig AE005174_GR.
Gene locus ECs3408 in contig BA000007_GR.
KEGGece:Z3814.
ecs:ECs3408.
PATRIC18356248. VBIEscCol44059_3319.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009316.
HOGENOMHBG644911.
OMAATQRGCK.
ProtClustDBPRK09754.

Enzyme and pathway databases

BioCycECOL83334:ECS3408-MONOMER.

Family and domain databases

HAMAPMF_01651. HcaD.
[Tree]
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023744. HcaD.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
Gene3DG3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KOK00529.
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHCAD_ECO57
AccessionPrimary (citable) accession number: Q8XA71
Secondary accession number(s): Q7ABL5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2002
Last modified: January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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