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Q8XA46 (PUR4_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:Z3835, ECs3423
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length1295 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12951295Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100408

Regions

Domain1042 – 1295254Glutamine amidotransferase type-1
Nucleotide binding307 – 31812ATP Potential

Sites

Active site11351Nucleophile By similarity
Active site12601 By similarity
Active site12621 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XA46 [UniParc].

Last modified December 4, 2007. Version 3.
Checksum: 86F7FF0B2C261AA1

FASTA1,295141,401
        10         20         30         40         50         60 
MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD EHAQLERLLK 

        70         80         90        100        110        120 
YGPALASHAP QGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVNRLERGV AYYIEAGTLT 

       130        140        150        160        170        180 
NEQWQQVTAE LHDRMMETVF FALDDAEQLF AHHQPTPVTS VDLLGQGRQA LIDANLRLGL 

       190        200        210        220        230        240 
ALAEDEIDYL QDAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWIIDG EQQPKSLFKM 

       250        260        270        280        290        300 
IKNTFETTPD HVLSAYKDNA AVMEGSEVGR YFADHETGRY DFHQEPAHIL MKVETHNHPT 

       310        320        330        340        350        360 
AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV 

       370        380        390        400        410        420 
TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI 

       430        440        450        460        470        480 
RADHVQKGEI NVGAKLVVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR 

       490        500        510        520        530        540 
CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELRDI LSDEPGMSPL 

       550        560        570        580        590        600 
EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL 

       610        620        630        640        650        660 
PLDVLLGKTP KMTRDVQTLK AKGDALVREG ITIADAVKRV LHLPTVAEKT FLVTIGDRSV 

       670        680        690        700        710        720 
TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER APVALLDFAA SARLAVGEAL 

       730        740        750        760        770        780 
TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 

       790        800        810        820        830        840 
MKTRWQEGNE EREMTSPLSL VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA 

       850        860        870        880        890        900 
TALAQVYRQL GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA 

       910        920        930        940        950        960 
GHCGIDADIA TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD CVHYVGQAVS 

       970        980        990       1000       1010       1020 
GDRFVITANG QTVFSESRTT LRVWWAETTW QMQRLRDNPE CADQEHQAKS NDADPGLNVK 

      1030       1040       1050       1060       1070       1080 
LSFDINEDVA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLAGRT 

      1090       1100       1110       1120       1130       1140 
GLEDFHALVA CGGFSYGDVL GAGEGWAKSI LFNDRVRDEF ATFFHRPQTL ALGVCNGCQM 

      1150       1160       1170       1180       1190       1200 
MSNLRELIPG SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR 

      1210       1220       1230       1240       1250       1260 
VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE SGRVTIMMPH 

      1270       1280       1290 
PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed: 11206551] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG57671.2.
BA000007 Genomic DNA. Translation: BAB36846.2.
PIRC85901.
G91056.
RefSeqNP_289113.1. NC_002655.2.
NP_311450.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8XA46.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000024861; EBESCP00000023754; EBESCG00000023915.
EBESCT00000058314; EBESCP00000056142; EBESCG00000057362.
GeneID914912.
957901.
GenomeReviewsGene locus Z3835 in contig AE005174_GR.
Gene locus ECs3423 in contig BA000007_GR.
KEGGece:Z3835.
ecs:ECs3423.
PATRIC18356282. VBIEscCol44059_3336.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000010521.
HOGENOMHBG335309.
OMAVKAVGME.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycECOL83334:ECS3423-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_ECO57
AccessionPrimary (citable) accession number: Q8XA46
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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