Reviewed, UniProtKB/Swiss-Prot Q8XA46 (PUR4_ECO57)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	Z3835, ECs3423

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100408
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XA46-1 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: 86F7FF0B2C261AA1	FASTA	1,295	141,401
	10 20 30 40 50 60 MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD EHAQLERLLK 70 80 90 100 110 120 YGPALASHAP QGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVNRLERGV AYYIEAGTLT 130 140 150 160 170 180 NEQWQQVTAE LHDRMMETVF FALDDAEQLF AHHQPTPVTS VDLLGQGRQA LIDANLRLGL 190 200 210 220 230 240 ALAEDEIDYL QDAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWIIDG EQQPKSLFKM 250 260 270 280 290 300 IKNTFETTPD HVLSAYKDNA AVMEGSEVGR YFADHETGRY DFHQEPAHIL MKVETHNHPT 310 320 330 340 350 360 AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV 370 380 390 400 410 420 TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI 430 440 450 460 470 480 RADHVQKGEI NVGAKLVVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR 490 500 510 520 530 540 CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELRDI LSDEPGMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL 610 620 630 640 650 660 PLDVLLGKTP KMTRDVQTLK AKGDALVREG ITIADAVKRV LHLPTVAEKT FLVTIGDRSV 670 680 690 700 710 720 TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER APVALLDFAA SARLAVGEAL 730 740 750 760 770 780 TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 790 800 810 820 830 840 MKTRWQEGNE EREMTSPLSL VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA 850 860 870 880 890 900 TALAQVYRQL GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA 910 920 930 940 950 960 GHCGIDADIA TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD CVHYVGQAVS 970 980 990 1000 1010 1020 GDRFVITANG QTVFSESRTT LRVWWAETTW QMQRLRDNPE CADQEHQAKS NDADPGLNVK 1030 1040 1050 1060 1070 1080 LSFDINEDVA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLAGRT 1090 1100 1110 1120 1130 1140 GLEDFHALVA CGGFSYGDVL GAGEGWAKSI LFNDRVRDEF ATFFHRPQTL ALGVCNGCQM 1150 1160 1170 1180 1190 1200 MSNLRELIPG SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR 1210 1220 1230 1240 1250 1260 VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE SGRVTIMMPH 1270 1280 1290 PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57671.2. BA000007 Genomic DNA. Translation: BAB36846.2.
PIR	C85901. G91056.
RefSeq	NP_289113.1. NP_311450.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	914912. 957901.
GenomeReviews	Gene locus Z3835 in contig AE005174_GR. Gene locus ECs3423 in contig BA000007_GR.
KEGG	ece:Z3835. ecs:ECs3423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335309.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3423-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_ECO57

Accession

Primary (citable) accession number: Q8XA46

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)