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Q8X9Y6 (DDLB_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase B
EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase B
D-alanylalanine synthetase B
Gene names
Name:ddlB
Ordered Locus Names:Z0102, ECs0096
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047

Subunit structure

Monomer By similarity. HAMAP MF_00047

Subcellular location

Cytoplasm By similarity HAMAP MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 306305D-alanine--D-alanine ligase B HAMAP MF_00047
PRO_0000177820

Regions

Domain101 – 303203ATP-grasp
Nucleotide binding134 – 18956ATP By similarity

Sites

Active site151 By similarity
Active site1501 By similarity
Active site2811 By similarity
Metal binding2571Magnesium or manganese 1 By similarity
Metal binding2701Magnesium or manganese 1 By similarity
Metal binding2701Magnesium or manganese 2 By similarity
Metal binding2721Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8X9Y6 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3302284E030DD647

FASTA30632,854
        10         20         30         40         50         60 
MTDKIAVLLG GTSAEREVSL NSGAAVLAGL REGGIDAYPV DPKEVDVTQL KSMGFQKVFI 

        70         80         90        100        110        120 
ALHGRGGEDG TLQGMLELMG LPYTGSGVMA SALSMDKLRS KLLWQGAGLP VAPWVALTRV 

       130        140        150        160        170        180 
EFEKGLSDKQ LAEISALGLP VIVKPSREGS SVGMSKVVAE NALQDALRLA FQHDEEVLIE 

       190        200        210        220        230        240 
KWLSGPEFTV AILGEEILPS VRIQPSGTFY DYEAKYLSDE TQYFCPAGLE ASQEANLQAL 

       250        260        270        280        290        300 
VLKAWTTLGC KGWGRIDVML DSDGQFYLLE ANTSPGMTSH SLVPMAARQA GMSFSQLVVR 


ILELAD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG54396.1.
BA000007 Genomic DNA. Translation: BAB33519.1.
PIRH85491.
H90640.
RefSeqNP_285788.1. NC_002655.2.
NP_308123.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8X9Y6.
SMRQ8X9Y6. Positions 1-306.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000027180; EBESCP00000026073; EBESCG00000026232.
EBESCT00000056869; EBESCP00000054697; EBESCG00000055917.
GeneID913558.
956778.
GenomeReviewsGene locus Z0102 in contig AE005174_GR.
Gene locus ECs0096 in contig BA000007_GR.
KEGGece:Z0102.
ecs:ECs0096.
PATRIC18349134. VBIEscCol44059_0097.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009081.
HOGENOMHBG427092.
OMAWREAARY.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycECOL83334:ECS0096-MONOMER.

Family and domain databases

HAMAPMF_00047. Dala_Dala_lig.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01921.
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLB_ECO57
AccessionPrimary (citable) accession number: Q8X9Y6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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