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Protein

Argininosuccinate synthase

Gene

argG

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argI)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei99CitrullineBy similarity1
Binding sitei129ATP; via amide nitrogenBy similarity1
Binding sitei131AspartateBy similarity1
Binding sitei131ATPBy similarity1
Binding sitei135AspartateBy similarity1
Binding sitei135CitrullineBy similarity1
Binding sitei136AspartateBy similarity1
Binding sitei136ATPBy similarity1
Binding sitei139CitrullineBy similarity1
Binding sitei192CitrullineBy similarity1
Binding sitei194ATPBy similarity1
Binding sitei201CitrullineBy similarity1
Binding sitei203CitrullineBy similarity1
Binding sitei280CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:Z4534, ECs4052
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001486962 – 447Argininosuccinate synthaseAdd BLAST446

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi155864.Z4534

Structurei

3D structure databases

ProteinModelPortaliQ8X9M0
SMRiQ8X9M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230094
KOiK01940
OMAiGVGRIDM

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di1.10.287.400, 1 hit
3.40.50.620, 2 hits
3.90.1260.10, 2 hits
HAMAPiMF_00581 Arg_succ_synth_type2, 1 hit
InterProiView protein in InterPro
IPR023437 Arg_succ_synth_type2_subfam
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR024073 AS_multimer_C_tail
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8X9M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTILKHLPV GQRIGIAFSG GLDTSAALLW MRQKGAVPYA YTANLGQPDE
60 70 80 90 100
EDYDAIPRRA MEYGAENARL IDCRKQLVAE GIAAIQCGAF HNTTGGLTYF
110 120 130 140 150
NTTPLGRAVT GTMLVAAMKE DGVNIWGDGS TYKGNDIERF YRYGLLTNAE
160 170 180 190 200
LQIYKPWLDT DFIDELGGRH EMSEFMIACG FDYKMSVEKA YSTDSNMLGA
210 220 230 240 250
THEAKDLEYL NSSVKIVNPI MGVKFWDESV KIPAEEVTVR FEQGHPVALN
260 270 280 290 300
GKTFSDDVEM MLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
HIAYERLLTG IHNEDTIEQY HAHGRQLGRL LYQGRWFDSQ ALMLRDSLQR
360 370 380 390 400
WVASQITGEV TLELRRGNDY SILNTVSENL TYKPERLTME KGDSVFSPDD
410 420 430 440
RIGQLTMRNL DITDTREKLF GYAKTGLLSS SAASGVPQME NLENKGQ
Length:447
Mass (Da):49,930
Last modified:January 23, 2007 - v3
Checksum:iC850E51BD06E1813
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58307.1
BA000007 Genomic DNA Translation: BAB37475.1
PIRiD91135
G85980
RefSeqiNP_312079.1, NC_002695.1
WP_000207678.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58307; AAG58307; Z4534
BAB37475; BAB37475; BAB37475
GeneIDi916108
KEGGiece:Z4534
ecs:ECs4052
PATRICifig|386585.9.peg.4231

Similar proteinsi

Entry informationi

Entry nameiASSY_ECO57
AccessioniPrimary (citable) accession number: Q8X9M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health