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Q8X929

- PANB_ECO57

UniProt

Q8X929 - PANB_ECO57

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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451MagnesiumUniRule annotation
Metal bindingi84 – 841MagnesiumUniRule annotation
Binding sitei84 – 841Alpha-ketoisovalerateUniRule annotation
Binding sitei112 – 1121Alpha-ketoisovalerateUniRule annotation
Metal bindingi114 – 1141MagnesiumUniRule annotation
Active sitei181 – 1811Proton acceptorUniRule annotation

GO - Molecular functioni

  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. pantothenate biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-135-MONOMER.
ECOO157:PANB-MONOMER.
UniPathwayiUPA00028; UER00003.

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:Z0145, ECs0138
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2642643-methyl-2-oxobutanoate hydroxymethyltransferasePRO_0000184843Add
BLAST

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z0145.

Structurei

3D structure databases

ProteinModelPortaliQ8X929.
SMRiQ8X929. Positions 3-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 462Alpha-ketoisovalerate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0413.
HOGENOMiHOG000078427.
KOiK00606.
OMAiEKIAMIT.
OrthoDBiEOG63C0WN.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_00156. PanB.
InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiPF02548. Pantoate_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8X929-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPTTIASLQ KCKQEKKRFA TITAYDYSFA KLFAEEGLNV MLVGDSLGMT
60 70 80 90 100
VQGHESTLPV TVEDIAYHTT AVRRGAPNCL LLADLPFMAY ATPEQAFENA
110 120 130 140 150
ATVMRAGANM VKIEGGEWLV ETVKMLTERA VPVCGHLGLT PQSVNIFGGY
160 170 180 190 200
KVQGRGDEAS DRLLSDALAL EAAGAQLLVL ECVPVELAKR ITEALAIPVI
210 220 230 240 250
GIGAGNVTDG QILVMHDAFG ITGGHIPKFA KNFLAETGDI RAAVRQYMAE
260
VESGVYPGEE HSFH
Length:264
Mass (Da):28,310
Last modified:March 1, 2002 - v1
Checksum:iD93FF23EBEF8D090
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54438.1.
BA000007 Genomic DNA. Translation: BAB33561.1.
PIRiB85497.
B90646.
RefSeqiNP_285830.1. NC_002655.2.
NP_308165.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54438; AAG54438; Z0145.
BAB33561; BAB33561; BAB33561.
GeneIDi913736.
956841.
KEGGiece:Z0145.
ecs:ECs0138.
PATRICi18349218. VBIEscCol44059_0139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54438.1 .
BA000007 Genomic DNA. Translation: BAB33561.1 .
PIRi B85497.
B90646.
RefSeqi NP_285830.1. NC_002655.2.
NP_308165.1. NC_002695.1.

3D structure databases

ProteinModelPortali Q8X929.
SMRi Q8X929. Positions 3-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z0145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG54438 ; AAG54438 ; Z0145 .
BAB33561 ; BAB33561 ; BAB33561 .
GeneIDi 913736.
956841.
KEGGi ece:Z0145.
ecs:ECs0138.
PATRICi 18349218. VBIEscCol44059_0139.

Phylogenomic databases

eggNOGi COG0413.
HOGENOMi HOG000078427.
KOi K00606.
OMAi EKIAMIT.
OrthoDBi EOG63C0WN.

Enzyme and pathway databases

UniPathwayi UPA00028 ; UER00003 .
BioCyci ECOL386585:GJFA-135-MONOMER.
ECOO157:PANB-MONOMER.

Family and domain databases

Gene3Di 3.20.20.60. 1 hit.
HAMAPi MF_00156. PanB.
InterProi IPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
PANTHERi PTHR20881. PTHR20881. 1 hit.
Pfami PF02548. Pantoate_transf. 1 hit.
[Graphical view ]
PIRSFi PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMi SSF51621. SSF51621. 1 hit.
TIGRFAMsi TIGR00222. panB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPANB_ECO57
AccessioniPrimary (citable) accession number: Q8X929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3