SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8X929

- PANB_ECO57

UniProt

Q8X929 - PANB_ECO57

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene
panB, Z0145, ECs0138
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Magnesium By similarity
Metal bindingi84 – 841Magnesium By similarity
Binding sitei84 – 841Alpha-ketoisovalerate By similarity
Binding sitei112 – 1121Alpha-ketoisovalerate By similarity
Metal bindingi114 – 1141Magnesium By similarity
Active sitei181 – 1811Proton acceptor By similarity

GO - Molecular functioni

  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. pantothenate biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-135-MONOMER.
ECOO157:PANB-MONOMER.
UniPathwayiUPA00028; UER00003.

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase (EC:2.1.2.11)
Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name:
KPHMT
Gene namesi
Name:panB
Ordered Locus Names:Z0145, ECs0138
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2642643-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotationPRO_0000184843Add
BLAST

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z0145.

Structurei

3D structure databases

ProteinModelPortaliQ8X929.
SMRiQ8X929. Positions 3-264.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 462Alpha-ketoisovalerate binding By similarity

Sequence similaritiesi

Belongs to the PanB family.

Phylogenomic databases

eggNOGiCOG0413.
HOGENOMiHOG000078427.
KOiK00606.
OMAiEKIAMIT.
OrthoDBiEOG63C0WN.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_00156. PanB.
InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiPF02548. Pantoate_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8X929-1 [UniParc]FASTAAdd to Basket

« Hide

MKPTTIASLQ KCKQEKKRFA TITAYDYSFA KLFAEEGLNV MLVGDSLGMT    50
VQGHESTLPV TVEDIAYHTT AVRRGAPNCL LLADLPFMAY ATPEQAFENA 100
ATVMRAGANM VKIEGGEWLV ETVKMLTERA VPVCGHLGLT PQSVNIFGGY 150
KVQGRGDEAS DRLLSDALAL EAAGAQLLVL ECVPVELAKR ITEALAIPVI 200
GIGAGNVTDG QILVMHDAFG ITGGHIPKFA KNFLAETGDI RAAVRQYMAE 250
VESGVYPGEE HSFH 264
Length:264
Mass (Da):28,310
Last modified:March 1, 2002 - v1
Checksum:iD93FF23EBEF8D090
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG54438.1.
BA000007 Genomic DNA. Translation: BAB33561.1.
PIRiB85497.
B90646.
RefSeqiNP_285830.1. NC_002655.2.
NP_308165.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54438; AAG54438; Z0145.
BAB33561; BAB33561; BAB33561.
GeneIDi913736.
956841.
KEGGiece:Z0145.
ecs:ECs0138.
PATRICi18349218. VBIEscCol44059_0139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG54438.1 .
BA000007 Genomic DNA. Translation: BAB33561.1 .
PIRi B85497.
B90646.
RefSeqi NP_285830.1. NC_002655.2.
NP_308165.1. NC_002695.1.

3D structure databases

ProteinModelPortali Q8X929.
SMRi Q8X929. Positions 3-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z0145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG54438 ; AAG54438 ; Z0145 .
BAB33561 ; BAB33561 ; BAB33561 .
GeneIDi 913736.
956841.
KEGGi ece:Z0145.
ecs:ECs0138.
PATRICi 18349218. VBIEscCol44059_0139.

Phylogenomic databases

eggNOGi COG0413.
HOGENOMi HOG000078427.
KOi K00606.
OMAi EKIAMIT.
OrthoDBi EOG63C0WN.

Enzyme and pathway databases

UniPathwayi UPA00028 ; UER00003 .
BioCyci ECOL386585:GJFA-135-MONOMER.
ECOO157:PANB-MONOMER.

Family and domain databases

Gene3Di 3.20.20.60. 1 hit.
HAMAPi MF_00156. PanB.
InterProi IPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
PANTHERi PTHR20881. PTHR20881. 1 hit.
Pfami PF02548. Pantoate_transf. 1 hit.
[Graphical view ]
PIRSFi PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMi SSF51621. SSF51621. 1 hit.
TIGRFAMsi TIGR00222. panB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPANB_ECO57
AccessioniPrimary (citable) accession number: Q8X929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi