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Q8X929

- PANB_ECO57

UniProt

Q8X929 - PANB_ECO57

Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451MagnesiumUniRule annotation
    Metal bindingi84 – 841MagnesiumUniRule annotation
    Binding sitei84 – 841Alpha-ketoisovalerateUniRule annotation
    Binding sitei112 – 1121Alpha-ketoisovalerateUniRule annotation
    Metal bindingi114 – 1141MagnesiumUniRule annotation
    Active sitei181 – 1811Proton acceptorUniRule annotation

    GO - Molecular functioni

    1. 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. pantothenate biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Pantothenate biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-135-MONOMER.
    ECOO157:PANB-MONOMER.
    UniPathwayiUPA00028; UER00003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
    Alternative name(s):
    Ketopantoate hydroxymethyltransferaseUniRule annotation
    Short name:
    KPHMTUniRule annotation
    Gene namesi
    Name:panBUniRule annotation
    Ordered Locus Names:Z0145, ECs0138
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2642643-methyl-2-oxobutanoate hydroxymethyltransferasePRO_0000184843Add
    BLAST

    Interactioni

    Subunit structurei

    Homodecamer; pentamer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi155864.Z0145.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X929.
    SMRiQ8X929. Positions 3-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni45 – 462Alpha-ketoisovalerate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PanB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0413.
    HOGENOMiHOG000078427.
    KOiK00606.
    OMAiEKIAMIT.
    OrthoDBiEOG63C0WN.

    Family and domain databases

    Gene3Di3.20.20.60. 1 hit.
    HAMAPiMF_00156. PanB.
    InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR20881. PTHR20881. 1 hit.
    PfamiPF02548. Pantoate_transf. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR00222. panB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8X929-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPTTIASLQ KCKQEKKRFA TITAYDYSFA KLFAEEGLNV MLVGDSLGMT    50
    VQGHESTLPV TVEDIAYHTT AVRRGAPNCL LLADLPFMAY ATPEQAFENA 100
    ATVMRAGANM VKIEGGEWLV ETVKMLTERA VPVCGHLGLT PQSVNIFGGY 150
    KVQGRGDEAS DRLLSDALAL EAAGAQLLVL ECVPVELAKR ITEALAIPVI 200
    GIGAGNVTDG QILVMHDAFG ITGGHIPKFA KNFLAETGDI RAAVRQYMAE 250
    VESGVYPGEE HSFH 264
    Length:264
    Mass (Da):28,310
    Last modified:March 1, 2002 - v1
    Checksum:iD93FF23EBEF8D090
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG54438.1.
    BA000007 Genomic DNA. Translation: BAB33561.1.
    PIRiB85497.
    B90646.
    RefSeqiNP_285830.1. NC_002655.2.
    NP_308165.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG54438; AAG54438; Z0145.
    BAB33561; BAB33561; BAB33561.
    GeneIDi913736.
    956841.
    KEGGiece:Z0145.
    ecs:ECs0138.
    PATRICi18349218. VBIEscCol44059_0139.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG54438.1 .
    BA000007 Genomic DNA. Translation: BAB33561.1 .
    PIRi B85497.
    B90646.
    RefSeqi NP_285830.1. NC_002655.2.
    NP_308165.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali Q8X929.
    SMRi Q8X929. Positions 3-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z0145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG54438 ; AAG54438 ; Z0145 .
    BAB33561 ; BAB33561 ; BAB33561 .
    GeneIDi 913736.
    956841.
    KEGGi ece:Z0145.
    ecs:ECs0138.
    PATRICi 18349218. VBIEscCol44059_0139.

    Phylogenomic databases

    eggNOGi COG0413.
    HOGENOMi HOG000078427.
    KOi K00606.
    OMAi EKIAMIT.
    OrthoDBi EOG63C0WN.

    Enzyme and pathway databases

    UniPathwayi UPA00028 ; UER00003 .
    BioCyci ECOL386585:GJFA-135-MONOMER.
    ECOO157:PANB-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.60. 1 hit.
    HAMAPi MF_00156. PanB.
    InterProi IPR003700. Pantoate_hydroxy_MeTrfase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR20881. PTHR20881. 1 hit.
    Pfami PF02548. Pantoate_transf. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR00222. panB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiPANB_ECO57
    AccessioniPrimary (citable) accession number: Q8X929
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3