Reviewed, UniProtKB/Swiss-Prot Q8X8L4 (DLHH_ECO57)

Names and origin

Protein names

Recommended name:
    Putative carboxymethylenebutenolidase
    EC=3.1.1.45
Alternative name(s):
    Dienelactone hydrolase
      Short name=DLH

Gene names

Name:	ysgA
Ordered Locus Names:	Z5352, ECs4760

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Putative carboxymethylenebutenolidase		PRO_0000161579
Sites
	Active site	147	1	By similarity
	Active site	204	1	By similarity
	Active site	236	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8X8L4-1 [UniParc]. Last modified February 21, 2002. Version 1. Checksum: A2FCEACBDC8F91E5	FASTA	271	29,396
	10 20 30 40 50 60 MATTQQSGFA PAASPLASTI VQTPDDAIVA GFTSIPSQGD NMPAYHARPK QSDGPLPVVI 70 80 90 100 110 120 VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND FADIPTLLSG LVAKVPDSQV 130 140 150 160 170 180 LADLDHVASW ASRNGGDVHR LMITGFCWGG RITWLYAAHN PQLKAAVAWY GKLTGDKSLN 190 200 210 220 230 240 SPKQPVDIAT DLNAPVLGLY GGLDNSIPQE SVETMRQALR AANAKAEIIV YPDAGHAFNA 250 260 270 DYRPSYHAAS AEDGWQRMLE WFKQYGGKKS L

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG59026.1. Different initiation. BA000007 Genomic DNA. Translation: BAB38183.1. Different initiation.
PIR	F86070. H91223.
RefSeq	NP_290462.1. NP_312787.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	915144. 960328.
GenomeReviews	Gene locus Z5352 in contig AE005174_GR. Gene locus ECs4760 in contig BA000007_GR.
KEGG	ece:Z5352. ecs:ECs4760.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8X8L4.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4760-MON.
Family and domain databases
InterPro	IPR002925. Dienelactn_hydro. [Graphical view]
Pfam	PF01738. DLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DLHH_ECO57

Accession

Primary (citable) accession number: Q8X8L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	December 16, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)