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Q8X7Z7 (DKGB_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,5-diketo-D-gluconic acid reductase B
Short name=2,5-DKG reductase B
Short name=2,5-DKGR B
Short name=25DKGR-B
EC=1.1.1.274
Alternative name(s):
AKR5D
Gene names
Name:dkgB
Ordered Locus Names:Z0229, ECs0203
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.

Catalytic activity

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Sequence similarities

Belongs to the aldo/keto reductase family.

Ontologies

Keywords
   Biological processAscorbate biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processL-ascorbic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function2,5-didehydrogluconate reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2672672,5-diketo-D-gluconic acid reductase B
PRO_0000124605

Regions

Nucleotide binding179 – 23153NADP By similarity

Sites

Active site391Proton donor By similarity
Binding site971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8X7Z7 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: C03126A69D94CED4

FASTA26729,439
        10         20         30         40         50         60 
MAIPAFGLGT FRLKDDVVIS SVKTALELGY RAIDTAQIYD NEAAVGLAIA ESGVPRHELY 

        70         80         90        100        110        120 
ITTKIWIENL SKDKLIPSLK ESLQKLRTDY VDLTLIHWPS PNDEVSVEEF MQALLEAKKE 

       130        140        150        160        170        180 
GLTREIGISN FTIPLMEKAI AAVGAENIAT NQIELSPYLQ NRKVVAWAKQ HGIHITSYMT 

       190        200        210        220        230        240 
LAYGKALKDE VIARIAAKHN ATPAQVILAW AMGEGYSVIP SSTKRENLES NLKAQNLQLD 

       250        260 
AEDKKAIAAL DCNDRLVSPE GLAPEWD

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed: 11206551] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
[3]"Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655."
Ohnishi M., Murata T., Nakayama K., Kuhara S., Hattori M., Kurokawa K., Yasunaga T., Yokoyama K., Makino K., Shinagawa H., Hayashi T.
Syst. Appl. Microbiol. 23:315-324(2000) [PubMed: 11108008] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-206.
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG54503.1.
BA000007 Genomic DNA. Translation: BAB33626.1.
AB035926 Genomic DNA. Translation: BAA93569.1.
PIRC85505.
C90654.
RefSeqNP_285895.1. NC_002655.2.
NP_308230.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8X7Z7.
SMRQ8X7Z7. Positions 1-255.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000029123; EBESCP00000028016; EBESCG00000028173.
EBESCT00000056702; EBESCP00000054530; EBESCG00000055750.
GeneID914031.
956971.
GenomeReviewsGene locus Z0229 in contig AE005174_GR.
Gene locus ECs0203 in contig BA000007_GR.
KEGGece:Z0229.
ecs:ECs0203.
PATRIC18349366. VBIEscCol44059_0207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009262.
HOGENOMHBG605727.
OMALAWAMQL.
ProtClustDBPRK11172.

Enzyme and pathway databases

BioCycECOL83334:ECS0203-MONOMER.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
KOK06222.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. Aldo/ket_red. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDKGB_ECO57
AccessionPrimary (citable) accession number: Q8X7Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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