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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41GTPUniRule annotation8
Nucleotide bindingi113 – 117GTPUniRule annotation5
Nucleotide bindingi168 – 171GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:Z4059, ECs3605
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915241 – 475Sulfate adenylyltransferase subunit 1Add BLAST475

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z4059

Structurei

3D structure databases

ProteinModelPortaliQ8X7X7
SMRiQ8X7X7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 239tr-type GAdd BLAST215

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 41G1By similarity8
Regioni92 – 96G2By similarity5
Regioni113 – 116G3By similarity4
Regioni168 – 171G4By similarity4
Regioni206 – 208G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3T Bacteria
COG2895 LUCA
HOGENOMiHOG000229289
KOiK00956
OMAiSRGDMIC

Family and domain databases

HAMAPiMF_00062 Sulf_adenylyltr_sub1, 1 hit
InterProiView protein in InterPro
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR011779 SO4_adenylTrfase_lsu
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02034 CysN, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8X7X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTALAQQIA NEGGVEAWMI AQQHKSLLRF LTCGSVDDGK STLIGRLLHD
60 70 80 90 100
TRQIYEDQLS SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR
110 120 130 140 150
YFSTEKRKFI IADTPGHEQY TRNMATGAST CELAILLIDA RKGVLDQTRR
160 170 180 190 200
HSFISTLLGI KHLVVAINKM DLVDYSEETF TRIREDYLTF AGQLPGNLDI
210 220 230 240 250
RFVPLSALEG DNVASQSESM PWYSGPTLLE VLETVEIQRV VDAQPMRFPV
260 270 280 290 300
QYVNRPNLDF RGYAGTLASG RVEVGQRVKV LPSGVESNVA RIVTFDGDRE
310 320 330 340 350
EAFAGEAITL VLTDEIDISR GDLLLAADEA LPAVQSASVD VVWMAEQPLS
360 370 380 390 400
PGQSYDIKIA GKKTRSRVDG IRYQVDINNL TQREVENLPL NGIGLVDLTF
410 420 430 440 450
DEPLVLDRYQ QNPVTGGLIF IDRLSNVTVG AGMVHEPVSQ ATAAPSEFSA
460 470
FELELNALVR RHFPHWGARD LLGEK
Length:475
Mass (Da):52,588
Last modified:March 1, 2002 - v1
Checksum:i61E833FB68C50B78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG57858.1
BA000007 Genomic DNA Translation: BAB37028.1
PIRiE91079
F85924
RefSeqiNP_311632.1, NC_002695.1
WP_001090366.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG57858; AAG57858; Z4059
BAB37028; BAB37028; BAB37028
GeneIDi914673
KEGGiece:Z4059
ecs:ECs3605
PATRICifig|386585.9.peg.3768

Similar proteinsi

Entry informationi

Entry nameiCYSN_ECO57
AccessioniPrimary (citable) accession number: Q8X7X7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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