Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41GTPUniRule annotation8
Nucleotide bindingi113 – 117GTPUniRule annotation5
Nucleotide bindingi168 – 171GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOO157:CYSN-MONOMER.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:Z4059, ECs3605
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915241 – 475Sulfate adenylyltransferase subunit 1Add BLAST475

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z4059.

Structurei

3D structure databases

ProteinModelPortaliQ8X7X7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 239tr-type GAdd BLAST215

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 41G1By similarity8
Regioni92 – 96G2By similarity5
Regioni113 – 116G3By similarity4
Regioni168 – 171G4By similarity4
Regioni206 – 208G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiFAANIVW.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8X7X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTALAQQIA NEGGVEAWMI AQQHKSLLRF LTCGSVDDGK STLIGRLLHD
60 70 80 90 100
TRQIYEDQLS SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR
110 120 130 140 150
YFSTEKRKFI IADTPGHEQY TRNMATGAST CELAILLIDA RKGVLDQTRR
160 170 180 190 200
HSFISTLLGI KHLVVAINKM DLVDYSEETF TRIREDYLTF AGQLPGNLDI
210 220 230 240 250
RFVPLSALEG DNVASQSESM PWYSGPTLLE VLETVEIQRV VDAQPMRFPV
260 270 280 290 300
QYVNRPNLDF RGYAGTLASG RVEVGQRVKV LPSGVESNVA RIVTFDGDRE
310 320 330 340 350
EAFAGEAITL VLTDEIDISR GDLLLAADEA LPAVQSASVD VVWMAEQPLS
360 370 380 390 400
PGQSYDIKIA GKKTRSRVDG IRYQVDINNL TQREVENLPL NGIGLVDLTF
410 420 430 440 450
DEPLVLDRYQ QNPVTGGLIF IDRLSNVTVG AGMVHEPVSQ ATAAPSEFSA
460 470
FELELNALVR RHFPHWGARD LLGEK
Length:475
Mass (Da):52,588
Last modified:March 1, 2002 - v1
Checksum:i61E833FB68C50B78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57858.1.
BA000007 Genomic DNA. Translation: BAB37028.1.
PIRiE91079.
F85924.
RefSeqiNP_311632.1. NC_002695.1.
WP_001090366.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG57858; AAG57858; Z4059.
BAB37028; BAB37028; BAB37028.
GeneIDi914673.
KEGGiece:Z4059.
ecs:ECs3605.
PATRICi18356683. VBIEscCol44059_3524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57858.1.
BA000007 Genomic DNA. Translation: BAB37028.1.
PIRiE91079.
F85924.
RefSeqiNP_311632.1. NC_002695.1.
WP_001090366.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliQ8X7X7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z4059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57858; AAG57858; Z4059.
BAB37028; BAB37028; BAB37028.
GeneIDi914673.
KEGGiece:Z4059.
ecs:ECs3605.
PATRICi18356683. VBIEscCol44059_3524.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiFAANIVW.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciECOO157:CYSN-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSN_ECO57
AccessioniPrimary (citable) accession number: Q8X7X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.