Reviewed, UniProtKB/Swiss-Prot Q8X7P1 (MANC1_ECO57)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanylyltransferase 1
    EC=2.7.7.13
Alternative name(s):
    GDP-mannose pyrophosphorylase 1
      Short name=GMPP 1
      Short name=GMP 1

Gene names

Name:	manC1
Synonyms:	cpsB
Ordered Locus Names:	Z3213, ECs2854

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. In vitro, shows very broad substrate specificities for four nucleotides and various hexose-1-phosphates, yielding ADP-mannose, CDP-mannose, UDP-mannose, GDP-mannose, GDP-glucose and GDP-2-deoxy-glucose. Ref.3
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose. Ref.3
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.5.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Mannose-1-phosphate guanylyltransferase 1		PRO_0000350734

Sequences

	Sequence		Length	Mass (Da)
	Q8X7P1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 46AAA47CEE00D861	FASTA	478	53,000
	10 20 30 40 50 60 MAQSKLYPVV MAGGSGSRLW PLSRVLYPKQ FLCLKGDLTM LQTTICRLNG VECESPVVIC 70 80 90 100 110 120 NEQHRFIVAE QLRQLNKLTE NIILEPAGRN TAPAIALAAL AAKRHSPEND PLMLVLAADH 130 140 150 160 170 180 VIADEDAFRA AVRNAMPYAE AGKLVTFGIV PDLPETGYGY IRRGEVSAGE QDTVAFEVAQ 190 200 210 220 230 240 FVEKPNLETA QAYVASGEYY WNSGMFLFRA GRYLEELKKY RPDILDACEK AMSAVDPDLD 250 260 270 280 290 300 FIRVDEDAFL ACPEESVDYA VMERTADAVV VPMDAGWSDV GSWSSLWEIS AHTAEGNVCH 310 320 330 340 350 360 GDVINHKTEN SYVYAESGLV TTVGVKDLVV VQTKDAVLIA DRNAVQDVKK VVEQIKADGR 370 380 390 400 410 420 HEHRVHREVY RPWGKYDSID AGDRYQVKRI TVKPGEGLSV QMHHHRAEHW VVVAGTAKVT 430 440 450 460 470 IDGDIKLLGE NESIYIPLGA THCLENPGKI PLDLIEVRSG SYLEEDDVVR FADRYGRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
[3]	"Characterization of GDP-mannose pyrophosphorylase from Escherichia coli O157:H7 EDL933 and its broad substrate specificity." Yang Y.-H., Kang Y.-B., Lee K.-W., Lee T.-H., Park S.-S., Hwang B.-Y., Kim B.-G. J. Mol. Catal., B Enzym. 37:1-8(2005) Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, PH DEPENDENCE. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG57109.1. BA000007 Genomic DNA. Translation: BAB36277.1.
PIR	A85831. F90985.
RefSeq	NP_288555.1. NP_310881.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	912555. 962108.
GenomeReviews	Gene locus Z3213 in contig AE005174_GR. Gene locus ECs2854 in contig BA000007_GR.
KEGG	ece:Z3213. ecs:ECs2854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8X7P1.
OMA	AEHRMVA.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2854-MONOMER.
Family and domain databases
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
ProDom	PD002664. Man6P_isomerII. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MANC1_ECO57

Accession

Primary (citable) accession number: Q8X7P1
Secondary accession number(s): Q7ACP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)