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Q8X7G3

- THIM_ECO57

UniProt

Q8X7G3 - THIM_ECO57

Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
    Binding sitei125 – 1251ATPUniRule annotation
    Binding sitei171 – 1711ATPUniRule annotation
    Binding sitei198 – 1981Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-2867-MONOMER.
    ECOO157:THIM-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
    Short name:
    TH kinaseUniRule annotation
    Short name:
    Thz kinaseUniRule annotation
    Gene namesi
    Name:thiMUniRule annotation
    Ordered Locus Names:Z3268, ECs2907
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Hydroxyethylthiazole kinasePRO_0000156935Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z3268.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X7G3.
    SMRiQ8X7G3. Positions 4-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    HOGENOMiHOG000114352.
    KOiK00878.
    OMAiQTFTANV.
    OrthoDBiEOG628F8M.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00694. thiM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8X7G3-1 [UniParc]FASTAAdd to Basket

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    MQVDLLSSAQ SAHTLHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM    50
    VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP 100
    VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGVANG GRGVDTTDAA 150
    VNAIPAAQTL ARETGAIVVV TGEVDYVTDG HRAVGIHGGD PLMTKVVGTG 200
    CALSAVVAAC CALPGDMLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL 250
    DALWQLTQEV QA 262
    Length:262
    Mass (Da):27,355
    Last modified:March 1, 2002 - v1
    Checksum:iA28BF773FD2AD29F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG57161.1.
    BA000007 Genomic DNA. Translation: BAB36330.1.
    PIRiC90992.
    E85837.
    RefSeqiNP_288606.1. NC_002655.2.
    NP_310934.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG57161; AAG57161; Z3268.
    BAB36330; BAB36330; BAB36330.
    GeneIDi916610.
    956840.
    KEGGiece:Z3268.
    ecs:ECs2907.
    PATRICi18355197. VBIEscCol44059_2799.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG57161.1 .
    BA000007 Genomic DNA. Translation: BAB36330.1 .
    PIRi C90992.
    E85837.
    RefSeqi NP_288606.1. NC_002655.2.
    NP_310934.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali Q8X7G3.
    SMRi Q8X7G3. Positions 4-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z3268.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG57161 ; AAG57161 ; Z3268 .
    BAB36330 ; BAB36330 ; BAB36330 .
    GeneIDi 916610.
    956840.
    KEGGi ece:Z3268.
    ecs:ECs2907.
    PATRICi 18355197. VBIEscCol44059_2799.

    Phylogenomic databases

    eggNOGi COG2145.
    HOGENOMi HOG000114352.
    KOi K00878.
    OMAi QTFTANV.
    OrthoDBi EOG628F8M.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci ECOL386585:GJFA-2867-MONOMER.
    ECOO157:THIM-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00694. thiM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiTHIM_ECO57
    AccessioniPrimary (citable) accession number: Q8X7G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3