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Protein

Gamma-glutamyl-gamma-aminobutyrate hydrolase

Gene

puuD

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma-aminobutyrate.By similarity

Catalytic activityi

4-(gamma-L-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate.

Pathwayi: putrescine degradation

This protein is involved in step 4 of the subpathway that synthesizes 4-aminobutanoate from putrescine.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Gamma-glutamyl-gamma-aminobutyrate hydrolase (puuD)
This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobutanoate from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114NucleophilePROSITE-ProRule annotation1
Active sitei222PROSITE-ProRule annotation1
Active sitei224PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00188; UER00883.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC:3.5.1.94)
Short name:
Gamma-Glu-GABA hydrolase
Gene namesi
Name:puuD
Ordered Locus Names:Z2490, ECs1875
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002726871 – 254Gamma-glutamyl-gamma-aminobutyrate hydrolaseAdd BLAST254

Interactioni

Protein-protein interaction databases

STRINGi155864.Z2490.

Structurei

3D structure databases

ProteinModelPortaliQ8X7G2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 250Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Belongs to the peptidase C26 family.Curated
Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108Z86. Bacteria.
COG2071. LUCA.
HOGENOMiHOG000035715.
KOiK09473.
OMAiGFITACR.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR011697. Peptidase_C26.
[Graphical view]
PfamiPF07722. Peptidase_C26. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8X7G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENIMNNPVI GVVMCRNRLK GHATQTLQEK YLNAIIHAGG LPIALPHALA
60 70 80 90 100
EPSLLEQLLP KLDGIYLPGS PSNVQPHLYG ENGDEPDADP ERDLLSMALI
110 120 130 140 150
NAALERRIPI FAICRGLQEL VVATGGSLHR KLCEQPELLE HREDPELPVE
160 170 180 190 200
QQYAPSHEVQ VEEGGLLSAL LPECSNFWVN SLHGQGAKVV SPRLRVEARS
210 220 230 240 250
PDGLVEAVSV INHPFALGVQ WHPEWNSSEY ALSRILFEGF ITACQHHIAE

KQRL
Length:254
Mass (Da):28,085
Last modified:January 23, 2007 - v2
Checksum:iB71D97A9C990F722
GO

Sequence cautioni

The sequence AAG56506 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB35298 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56506.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB35298.1. Different initiation.
PIRiC90863.
F85755.
RefSeqiNP_309902.2. NC_002695.1.
WP_001322278.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG56506; AAG56506; Z2490.
BAB35298; BAB35298; BAB35298.
GeneIDi912701.
KEGGiece:Z2490.
ecs:ECs1875.
PATRICi18353635. VBIEscCol44059_2034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56506.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB35298.1. Different initiation.
PIRiC90863.
F85755.
RefSeqiNP_309902.2. NC_002695.1.
WP_001322278.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliQ8X7G2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z2490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56506; AAG56506; Z2490.
BAB35298; BAB35298; BAB35298.
GeneIDi912701.
KEGGiece:Z2490.
ecs:ECs1875.
PATRICi18353635. VBIEscCol44059_2034.

Phylogenomic databases

eggNOGiENOG4108Z86. Bacteria.
COG2071. LUCA.
HOGENOMiHOG000035715.
KOiK09473.
OMAiGFITACR.

Enzyme and pathway databases

UniPathwayiUPA00188; UER00883.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR011697. Peptidase_C26.
[Graphical view]
PfamiPF07722. Peptidase_C26. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUUD_ECO57
AccessioniPrimary (citable) accession number: Q8X7G2
Secondary accession number(s): Q7AEJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.