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Protein

Gamma-glutamyl-gamma-aminobutyrate hydrolase

Gene

puuD

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma-aminobutyrate.By similarity

Catalytic activityi

4-(gamma-L-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate.

Pathwayi: putrescine degradation

This protein is involved in step 4 of the subpathway that synthesizes 4-aminobutanoate from putrescine.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Gamma-glutamyl-gamma-aminobutyrate hydrolase (puuD)
This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobutanoate from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114NucleophilePROSITE-ProRule annotation1
Active sitei222PROSITE-ProRule annotation1
Active sitei224PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

UniPathwayiUPA00188; UER00883

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC:3.5.1.94)
Short name:
Gamma-Glu-GABA hydrolase
Gene namesi
Name:puuD
Ordered Locus Names:Z2490, ECs1875
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002726871 – 254Gamma-glutamyl-gamma-aminobutyrate hydrolaseAdd BLAST254

Interactioni

Protein-protein interaction databases

STRINGi155864.Z2490

Structurei

3D structure databases

ProteinModelPortaliQ8X7G2
SMRiQ8X7G2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 250Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Belongs to the peptidase C26 family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108Z86 Bacteria
COG2071 LUCA
HOGENOMiHOG000035715
KOiK09473
OMAiNVEPHRY

Family and domain databases

Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR011697 Peptidase_C26
PfamiView protein in Pfam
PF07722 Peptidase_C26, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8X7G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENIMNNPVI GVVMCRNRLK GHATQTLQEK YLNAIIHAGG LPIALPHALA
60 70 80 90 100
EPSLLEQLLP KLDGIYLPGS PSNVQPHLYG ENGDEPDADP ERDLLSMALI
110 120 130 140 150
NAALERRIPI FAICRGLQEL VVATGGSLHR KLCEQPELLE HREDPELPVE
160 170 180 190 200
QQYAPSHEVQ VEEGGLLSAL LPECSNFWVN SLHGQGAKVV SPRLRVEARS
210 220 230 240 250
PDGLVEAVSV INHPFALGVQ WHPEWNSSEY ALSRILFEGF ITACQHHIAE

KQRL
Length:254
Mass (Da):28,085
Last modified:January 23, 2007 - v2
Checksum:iB71D97A9C990F722
GO

Sequence cautioni

The sequence AAG56506 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB35298 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG56506.1 Different initiation.
BA000007 Genomic DNA Translation: BAB35298.1 Different initiation.
PIRiC90863
F85755
RefSeqiNP_309902.2, NC_002695.1
WP_001322278.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG56506; AAG56506; Z2490
BAB35298; BAB35298; BAB35298
GeneIDi912701
KEGGiece:Z2490
ecs:ECs1875
PATRICifig|386585.9.peg.1978

Similar proteinsi

Entry informationi

Entry nameiPUUD_ECO57
AccessioniPrimary (citable) accession number: Q8X7G2
Secondary accession number(s): Q7AEJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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