Reviewed, UniProtKB/Swiss-Prot Q8X7A5 (CDH_ECO57)

Names and origin

Protein names

Recommended name:
    CDP-diacylglycerol pyrophosphatase
    EC=3.6.1.26
Alternative name(s):
    CDP-diacylglycerol phosphatidylhydrolase
    CDP-diglyceride hydrolase

Gene names

Name:	cdh
Ordered Locus Names:	Z5463, ECs4843

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	CDP-diacylglycerol + H2O = CMP + phosphatidate. HAMAP MF_00319
Pathway	Phospholipid metabolism; CDP-diacylglycerol degradation; phosphatidate from CDP-diacylglycerol: step 1/1. HAMAP MF_00319
Subcellular location	Cell inner membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the cdh family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CDP-diacylglycerol diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 251

251

CDP-diacylglycerol pyrophosphatase HAMAP MF_00319

PRO_0000198575

Regions

Transmembrane

4 – 24

21

Potential

Secondary structure

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251

Helix Strand Turn

Details...

Helix

33 – 40

8

Helix

42 – 49

8

Beta strand

55 – 58

4

Turn

60 – 62

3

Beta strand

63 – 68

6

Beta strand

70 – 74

5

Beta strand

76 – 82

7

Helix

90 – 93

4

Helix

100 – 106

7

Helix

109 – 115

7

Helix

121 – 123

3

Beta strand

124 – 129

6

Helix

131 – 133

3

Beta strand

141 – 146

6

Helix

148 – 155

8

Helix

156 – 160

5

Beta strand

178 – 182

5

Helix

184 – 189

6

Helix

192 – 199

8

Turn

201 – 205

5

Helix

207 – 209

3

Beta strand

210 – 216

7

Beta strand

222 – 228

7

Turn

231 – 234

4

Helix

239 – 242

4

Sequences

	Sequence		Length	Mass (Da)
	Q8X7A5-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C987D3A4B12BE5F3	FASTA	251	28,383
	10 20 30 40 50 60 MKKAGLLFLV MIVIAVVAAG IGYWKLTGEE SDTLRKIVLE ECLPNQQQNQ NPSPCAEVKP 70 80 90 100 110 120 NAGYVVLKDL NGPLQYLLMP TYRINGTESP LLTDPSTPNF FWLAWQARDF MSKKYGQPVP 130 140 150 160 170 180 DRAVSLAINS RTGRTQNHFH IHISCIRPDV REQLDNNLAN ISSRWLPLPG GLRGHEYLAR 190 200 210 220 230 240 RVTESELVQR SPFMMLAEEV PEAREHMGSY GLAMVRQSDN SFVLLATQRN LLTLNRASAE 250 EIQDHQCEIL R

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE005174 Genomic DNA. Translation: AAG59111.1.
BA000007 Genomic DNA. Translation: BAB38266.1.

PIR

C86081.
C91234.

RefSeq

NP_290547.1.
NP_312870.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2POF	X-ray	1.40	A/B	27-251	[»]

ModBase

Search...

Genome annotation databases

GeneID

915054.
960226.

GenomeReviews

Gene locus Z5463 in contig AE005174_GR.
Gene locus ECs4843 in contig BA000007_GR.

KEGG

ece:Z5463.
ecs:ECs4843.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q8X7A5.

Enzyme and pathway databases

BioCyc

ECOL83334:ECS4843-MON.

Family and domain databases

HAMAP

MF_00319.
[Tree]

InterPro

IPR003763. CDP-diacylglyc_Pase_bac.
IPR015993. CDP-diacylglyc_Pase_proteobac.
[Graphical view]

Pfam

PF02611. CDH. 1 hit.
[Graphical view]

PIRSF

PIRSF001273. CDH. 1 hit.

TIGRFAMs

TIGR00672. cdh. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CDH_ECO57

Accession

Primary (citable) accession number: Q8X7A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)