Q8X708 (Q8X708_ECO57) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phosphorylase RuleBase RU000587
EC=2.4.1.1 RuleBase RU000587

Gene names

Name:	malP EMBL AAG58518.1
Ordered Locus Names:	ECs4259 EMBL BAB37682.1, Z4772 EMBL AAG58518.1

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties By similarity. RuleBase RU000587
Catalytic activity	(1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate. RuleBase RU000587
Cofactor	Pyridoxal phosphate By similarity. RuleBase RU000587
Sequence similarities	Belongs to the glycogen phosphorylase family. RuleBase RU004179

Ontologies

Keywords
Biological process	Carbohydrate metabolism RuleBase RU000587
Molecular function	Glycosyltransferase RuleBase RU000587 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glycogen phosphorylase activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8X708 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0C163C8B8066EC5C	FASTA	797	90,522
	10 20 30 40 50 60 MSQPIFNDKQ FQEALSRQWQ RYGLNSAAEM TPRQWWLAVS EALAEMLRAQ PFAKPVANQR 70 80 90 100 110 120 HVNYISMEFL IGRLTGNNLL NLGWYQDVQD SLKAYDINLT DLLEEEIDPA LGNGGLGRLA 130 140 150 160 170 180 ACFLDSMATV GQSATGYGLN YQYGLFRQSF VDGKQVEAPD DWHRSNYPWF RHNEALDVQV 190 200 210 220 230 240 GIGGKVTKDG RWEPEFTITG QAWDLPVVGY RNGVAQPLRL WQATHAHPFD LTKFNDGDFL 250 260 270 280 290 300 RAEQQGINAE KLTKVLYPND NHTAGKKLRL MQQYFQCACS VADILRRHHL AGRKLHELAD 310 320 330 340 350 360 YEVIQLNDTH PTIAIPELLR VLIDEHQMSW DDAWAITSKT FAYTNHTLMP EALERWDVKL 370 380 390 400 410 420 VKGLLPRHMQ IINEINTRFK TLVEKTWPGD EKVWAKLAVV HDKQVHMANL CVVGGFAVNG 430 440 450 460 470 480 VAALHSDLVV KDLFPEYHQL WPNKFHNVTN GITPRRWIKQ CNPALAALLD KSLKKEWAND 490 500 510 520 530 540 LDQLINLEKF ADDAKFRQQY REIKQANKVR LAEFVKVRTG IEINPQAIFD IQIKRLHEYK 550 560 570 580 590 600 RQHLNLLHIL ALYKEIRENP QADRVPRVFL FGAKAAPGYY LAKNIIFAIN KVADVINNDP 610 620 630 640 650 660 LVGDKLKVVF LPDYCVSAAE KLIPAADISE QISTAGKEAS GTGNMKLALN GALTVGTLDG 670 680 690 700 710 720 ANVEIAEKVG EENIFIFGHT VEQVKAILAK GYDPVKWRKK DKVLDAVLKE LESGKYSDGD 730 740 750 760 770 780 KHAFDQMLHS IGKQGGDPYL VMADFAAYVE AQKQVDVLYR DQEAWTRAAI LNTARCGMFS 790 SDRSIRDYQA RIWQAKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak." Makino K., Yokoyama K., Kubota Y., Yutsudo C.H., Kimura S., Kurokawa K., Ishii K., Hattori M., Tatsuno I., Abe H., Iida T., Yamamoto K., Ohnishi M., Hayashi T., Yasunaga T., Honda T., Sasakawa C., Shinagawa H. Genes Genet. Syst. 74:227-239(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sakai EMBL BAB37682.1.
[2]	"Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak." Yokoyama K., Makino K., Kubota Y., Watanabe M., Kimura S., Yutsudo C.H., Kurokawa K., Ishii K., Hattori M., Abe H., Iida T., Yamamoto K., Hayashi T., Yasunaga T., Honda T., Sasakawa C., Shinagawa H. Gene 258:127-139(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sakai EMBL BAB37682.1.
[3]	"Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655." Ohnishi M., Murata T., Nakayama K., Kuhara S., Hattori M., Kurokawa K., Yasunaga T., Yokoyama K., Makino K., Shinagawa H., Hayashi T. Syst. Appl. Microbiol. 23:315-324(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sakai EMBL BAB37682.1.
[4]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC and Sakai EMBL BAB37682.1.
[5]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EDL933 EMBL AAG58518.1 and O157:H7 / EDL933 / ATCC 700927 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58518.1. BA000007 Genomic DNA. Translation: BAB37682.1.
PIR	B86007. C91161.
RefSeq	NP_289957.1. NC_002655.2. NP_312286.1. NC_002695.1.
3D structure databases
SMR	Q8X708. Positions 2-797.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z4772.
Protein family/group databases
CAZy	GT35. Glycosyltransferase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG58518; AAG58518; Z4772. BAB37682; BAB37682; BAB37682.
GeneID	915885. 958898.
KEGG	ece:Z4772. ecs:ECs4259.
PATRIC	18358081. VBIEscCol44059_4207.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000278445.
KO	K00688.
OMA	TESFDFY.
ProtClustDB	PRK14985.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-4229-MONOMER.
Family and domain databases
InterPro	IPR011833. Glycg_phsphrylas. IPR000811. Glyco_trans_35. [Graphical view]
PANTHER	PTHR11468. PTHR11468. 1 hit.
Pfam	PF00343. Phosphorylase. 1 hit. [Graphical view]
PIRSF	PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMs	TIGR02093. P_ylase. 1 hit.
PROSITE	PS00102. PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8X708_ECO57

Accession

Primary (citable) accession number: Q8X708
Secondary accession number(s): Q7AAA8

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)