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Q8X707

- RUSA_ECO57

UniProt

Q8X707 - RUSA_ECO57

Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701MagnesiumBy similarity
    Metal bindingi72 – 721MagnesiumBy similarity
    Metal bindingi91 – 911MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-1601-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    Ordered Locus Names:Z1873, ECs1619
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Crossover junction endodeoxyribonuclease RusAPRO_0000324835Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi155864.Z1873.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X707.
    SMRiQ8X707. Positions 1-118.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 164DNA bindingBy similarity
    Regioni66 – 738DNA bindingBy similarity

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Phylogenomic databases

    eggNOGiCOG4570.
    HOGENOMiHOG000119986.
    KOiK01160.
    OrthoDBiEOG66HVH9.

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001007. RusA. 1 hit.
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8X707-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTYSITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG    50
    LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV 100
    KMPVTKGGKL ELTITELGNE 120
    Length:120
    Mass (Da):13,773
    Last modified:March 1, 2002 - v1
    Checksum:i5E6B65CA32230A7F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55970.1.
    BA000007 Genomic DNA. Translation: BAB35042.1.
    PIRiC90831.
    F85688.
    RefSeqiNP_287358.1. NC_002655.2.
    NP_309646.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG55970; AAG55970; Z1873.
    BAB35042; BAB35042; BAB35042.
    GeneIDi913268.
    959829.
    KEGGiece:Z1873.
    ecs:ECs1619.
    PATRICi18352471. VBIEscCol44059_1460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55970.1 .
    BA000007 Genomic DNA. Translation: BAB35042.1 .
    PIRi C90831.
    F85688.
    RefSeqi NP_287358.1. NC_002655.2.
    NP_309646.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali Q8X707.
    SMRi Q8X707. Positions 1-118.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z1873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG55970 ; AAG55970 ; Z1873 .
    BAB35042 ; BAB35042 ; BAB35042 .
    GeneIDi 913268.
    959829.
    KEGGi ece:Z1873.
    ecs:ECs1619.
    PATRICi 18352471. VBIEscCol44059_1460.

    Phylogenomic databases

    eggNOGi COG4570.
    HOGENOMi HOG000119986.
    KOi K01160.
    OrthoDBi EOG66HVH9.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-1601-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001007. RusA. 1 hit.
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiRUSA_ECO57
    AccessioniPrimary (citable) accession number: Q8X707
    Secondary accession number(s): Q7AF07
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Is part of the cryptic phage CP-933X.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3