Q8X707 (RUSA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RusA
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase

Gene names

Name:	rusA
Ordered Locus Names:	Z1873, ECs1619

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Miscellaneous	Is part of the cryptic phage CP-933X.
Sequence similarities	Belongs to the RusA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	Crossover junction endodeoxyribonuclease RusA		PRO_0000324835
Regions
	Region	13 – 16	4	DNA binding By similarity
	Region	66 – 73	8	DNA binding By similarity
Sites
	Metal binding	70	1	Magnesium By similarity
	Metal binding	72	1	Magnesium By similarity
	Metal binding	91	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8X707 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5E6B65CA32230A7F	FASTA	120	13,773
	10 20 30 40 50 60 MNTYSITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG LAMPVKIRIE 70 80 90 100 110 120 CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGKL ELTITELGNE

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG55970.1. BA000007 Genomic DNA. Translation: BAB35042.1.
PIR	C90831. F85688.
RefSeq	NP_287358.1. NC_002655.2. NP_309646.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8X707.
SMR	Q8X707. Positions 1-118.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z1873.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG55970; AAG55970; Z1873. BAB35042; BAB35042; BAB35042.
GeneID	913268. 959829.
KEGG	ece:Z1873. ecs:ECs1619.
PATRIC	18352471. VBIEscCol44059_1460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4570.
HOGENOM	HOG000119986.
KO	K01160.
ProtClustDB	PRK09786.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-1601-MONOMER.
Family and domain databases
Gene3D	3.30.1330.70. 1 hit.
InterPro	IPR016281. Endonuclease_RusA. IPR008822. Endonuclease_RusA-like. [Graphical view]
Pfam	PF05866. RusA. 1 hit. [Graphical view]
PIRSF	PIRSF001007. RusA. 1 hit.
SUPFAM	SSF103084. RusA-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUSA_ECO57

Accession

Primary (citable) accession number: Q8X707
Secondary accession number(s): Q7AF07

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program