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Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC (By similarity).By similarity

Miscellaneous

Is part of the cryptic phage CP-933X.

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70MagnesiumBy similarity1
Metal bindingi72MagnesiumBy similarity1
Metal bindingi91MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene namesi
Name:rusA
Ordered Locus Names:Z1873, ECs1619
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003248351 – 120Crossover junction endodeoxyribonuclease RusAAdd BLAST120

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z1873

Structurei

3D structure databases

ProteinModelPortaliQ8X707
SMRiQ8X707
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 16DNA bindingBy similarity4
Regioni66 – 73DNA bindingBy similarity8

Sequence similaritiesi

Belongs to the RusA family.Curated

Phylogenomic databases

eggNOGiENOG4106CZA Bacteria
COG4570 LUCA
HOGENOMiHOG000119986
KOiK01160
OMAiKEQMLDI

Family and domain databases

Gene3Di3.30.1330.70, 1 hit
InterProiView protein in InterPro
IPR016281 Endonuclease_RusA
IPR008822 Endonuclease_RusA-like
IPR036614 RusA-like_sf
PfamiView protein in Pfam
PF05866 RusA, 1 hit
PIRSFiPIRSF001007 RusA, 1 hit
SUPFAMiSSF103084 SSF103084, 1 hit

Sequencei

Sequence statusi: Complete.

Q8X707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTYSITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV
110 120
KMPVTKGGKL ELTITELGNE
Length:120
Mass (Da):13,773
Last modified:March 1, 2002 - v1
Checksum:i5E6B65CA32230A7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG55970.1
BA000007 Genomic DNA Translation: BAB35042.1
PIRiC90831
F85688
RefSeqiNP_309646.1, NC_002695.1
WP_001099695.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG55970; AAG55970; Z1873
BAB35042; BAB35042; BAB35042
GeneIDi913268
KEGGiece:Z1873
ecs:ECs1619
PATRICifig|386585.9.peg.1717

Similar proteinsi

Entry informationi

Entry nameiRUSA_ECO57
AccessioniPrimary (citable) accession number: Q8X707
Secondary accession number(s): Q7AF07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health