Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8X707 (RUSA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RusA

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene names
Name:rusA
Ordered Locus Names:Z1873, ECs1619
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Miscellaneous

Is part of the cryptic phage CP-933X.

Sequence similarities

Belongs to the RusA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120Crossover junction endodeoxyribonuclease RusA
PRO_0000324835

Regions

Region13 – 164DNA binding By similarity
Region66 – 738DNA binding By similarity

Sites

Metal binding701Magnesium By similarity
Metal binding721Magnesium By similarity
Metal binding911Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8X707 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 5E6B65CA32230A7F

FASTA12013,773
        10         20         30         40         50         60 
MNTYSITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG LAMPVKIRIE 

        70         80         90        100        110        120 
CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGKL ELTITELGNE 

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG55970.1.
BA000007 Genomic DNA. Translation: BAB35042.1.
PIRC90831.
F85688.
RefSeqNP_287358.1. NC_002655.2.
NP_309646.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8X707.
SMRQ8X707. Positions 1-118.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z1873.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG55970; AAG55970; Z1873.
BAB35042; BAB35042; BAB35042.
GeneID913268.
959829.
KEGGece:Z1873.
ecs:ECs1619.
PATRIC18352471. VBIEscCol44059_1460.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4570.
HOGENOMHOG000119986.
KOK01160.
OrthoDBEOG66HVH9.
ProtClustDBPRK09786.

Enzyme and pathway databases

BioCycECOL386585:GJFA-1601-MONOMER.

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
PIRSFPIRSF001007. RusA. 1 hit.
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUSA_ECO57
AccessionPrimary (citable) accession number: Q8X707
Secondary accession number(s): Q7AF07
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families