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Q8X707

- RUSA_ECO57

UniProt

Q8X707 - RUSA_ECO57

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Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701MagnesiumBy similarity
Metal bindingi72 – 721MagnesiumBy similarity
Metal bindingi91 – 911MagnesiumBy similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-1601-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene namesi
Name:rusA
Ordered Locus Names:Z1873, ECs1619
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Crossover junction endodeoxyribonuclease RusAPRO_0000324835Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z1873.

Structurei

3D structure databases

ProteinModelPortaliQ8X707.
SMRiQ8X707. Positions 1-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 164DNA bindingBy similarity
Regioni66 – 738DNA bindingBy similarity

Sequence similaritiesi

Belongs to the RusA family.Curated

Phylogenomic databases

eggNOGiCOG4570.
HOGENOMiHOG000119986.
KOiK01160.
OrthoDBiEOG66HVH9.

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamiPF05866. RusA. 1 hit.
[Graphical view]
PIRSFiPIRSF001007. RusA. 1 hit.
SUPFAMiSSF103084. SSF103084. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8X707-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTYSITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV
110 120
KMPVTKGGKL ELTITELGNE
Length:120
Mass (Da):13,773
Last modified:March 1, 2002 - v1
Checksum:i5E6B65CA32230A7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG55970.1.
BA000007 Genomic DNA. Translation: BAB35042.1.
PIRiC90831.
F85688.
RefSeqiNP_287358.1. NC_002655.2.
NP_309646.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG55970; AAG55970; Z1873.
BAB35042; BAB35042; BAB35042.
GeneIDi913268.
959829.
KEGGiece:Z1873.
ecs:ECs1619.
PATRICi18352471. VBIEscCol44059_1460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG55970.1 .
BA000007 Genomic DNA. Translation: BAB35042.1 .
PIRi C90831.
F85688.
RefSeqi NP_287358.1. NC_002655.2.
NP_309646.1. NC_002695.1.

3D structure databases

ProteinModelPortali Q8X707.
SMRi Q8X707. Positions 1-118.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z1873.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG55970 ; AAG55970 ; Z1873 .
BAB35042 ; BAB35042 ; BAB35042 .
GeneIDi 913268.
959829.
KEGGi ece:Z1873.
ecs:ECs1619.
PATRICi 18352471. VBIEscCol44059_1460.

Phylogenomic databases

eggNOGi COG4570.
HOGENOMi HOG000119986.
KOi K01160.
OrthoDBi EOG66HVH9.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-1601-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.70. 1 hit.
InterProi IPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view ]
Pfami PF05866. RusA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001007. RusA. 1 hit.
SUPFAMi SSF103084. SSF103084. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiRUSA_ECO57
AccessioniPrimary (citable) accession number: Q8X707
Secondary accession number(s): Q7AF07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2002
Last modified: November 26, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Is part of the cryptic phage CP-933X.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3