Q8X6J0 (YAGS_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit
EC=1.17.1.4

Gene names

Name:	yagS
Ordered Locus Names:	Z0351, ECs0315

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	FAD By similarity.
Subunit structure	Heterotrimer of YagR, YagS and YagT Potential.
Sequence similarities	Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Putative xanthine dehydrogenase YagS FAD-binding subunit		PRO_0000166095
Regions
	Domain	1 – 223	223	FAD-binding PCMH-type
	Nucleotide binding	26 – 33	8	FAD Potential
	Nucleotide binding	107 – 111	5	FAD Potential
Sites
	Binding site	212	1	FAD; via amide nitrogen and carbonyl oxygen Potential
	Binding site	227	1	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8X6J0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 32C48CE9D0F16F90	FASTA	318	33,886
	10 20 30 40 50 60 MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID VNGLGLDKIE 70 80 90 100 110 120 VTDAGGLRIG ALVRNTDLVA HERVRRDYAV LSRALLAGAS GQLRNQATTA GNLLQRTRCP 130 140 150 160 170 180 YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV VGVSEACIAT HPSDMAVAMR LLDAVVETIT 190 200 210 220 230 240 PEGKTRSITL ADFYHPPGKT PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYTFA 250 260 270 280 290 300 LVSVAAIIQP DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDALFASA HPTAENTFKL 310 LLAKRTLASV LAEARAQA

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54610.1. BA000007 Genomic DNA. Translation: BAB33738.1.
PIR	C90668. F85518.
RefSeq	NP_286002.1. NC_002655.2. NP_308342.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8X6J0.
SMR	Q8X6J0. Positions 1-318.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z0351.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG54610; AAG54610; Z0351. BAB33738; BAB33738; BAB33738.
GeneID	914414. 957139.
KEGG	ece:Z0351. ecs:ECs0315.
PATRIC	18349572. VBIEscCol44059_0308.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1319.
HOGENOM	HOG000244727.
KO	K11178.
OMA	IGGFSRQ.
ProtClustDB	CLSK879612.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-315-MONOMER.
Family and domain databases
Gene3D	3.30.43.10. 1 hit. 3.30.465.10. 2 hits.
InterPro	IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR002346. Mopterin_DH_FAD-bd. [Graphical view]
Pfam	PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. [Graphical view]
SMART	SM01092. CO_deh_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF55447. CO_deh_flav_C. 1 hit. SSF56176. FAD-binding_2. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAGS_ECO57

Accession

Primary (citable) accession number: Q8X6J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program