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Protein

Putative xanthine dehydrogenase YagS FAD-binding subunit

Gene

yagS

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121FAD; via amide nitrogen and carbonyl oxygenSequence Analysis
Binding sitei227 – 2271FADSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338FADSequence Analysis
Nucleotide bindingi107 – 1115FADSequence Analysis

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  3. xanthine dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. purine nucleobase metabolic process Source: UniProtKB-KW
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism, Purine salvage

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciECOL386585:GJFA-315-MONOMER.
ECOO157:YAGS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit (EC:1.17.1.4)
Gene namesi
Name:yagS
Ordered Locus Names:Z0351, ECs0315
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558 Componenti: Chromosome UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Putative xanthine dehydrogenase YagS FAD-binding subunitPRO_0000166095Add
BLAST

Proteomic databases

PRIDEiQ8X6J0.

Interactioni

Subunit structurei

Heterotrimer of YagR, YagS and YagT.Curated

Protein-protein interaction databases

STRINGi155864.Z0351.

Structurei

3D structure databases

ProteinModelPortaliQ8X6J0.
SMRiQ8X6J0. Positions 1-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 223223FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1319.
HOGENOMiHOG000244727.
KOiK11178.
OMAiIGGFSRQ.
OrthoDBiEOG66TG3V.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8X6J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID
60 70 80 90 100
VNGLGLDKIE VTDAGGLRIG ALVRNTDLVA HERVRRDYAV LSRALLAGAS
110 120 130 140 150
GQLRNQATTA GNLLQRTRCP YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV
160 170 180 190 200
VGVSEACIAT HPSDMAVAMR LLDAVVETIT PEGKTRSITL ADFYHPPGKT
210 220 230 240 250
PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYTFA LVSVAAIIQP
260 270 280 290 300
DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDALFASA HPTAENTFKL
310
LLAKRTLASV LAEARAQA
Length:318
Mass (Da):33,886
Last modified:March 1, 2002 - v1
Checksum:i32C48CE9D0F16F90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54610.1.
BA000007 Genomic DNA. Translation: BAB33738.1.
PIRiC90668.
F85518.
RefSeqiNP_286002.1. NC_002655.2.
NP_308342.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54610; AAG54610; Z0351.
BAB33738; BAB33738; BAB33738.
GeneIDi914414.
957139.
KEGGiece:Z0351.
ecs:ECs0315.
PATRICi18349572. VBIEscCol44059_0308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54610.1.
BA000007 Genomic DNA. Translation: BAB33738.1.
PIRiC90668.
F85518.
RefSeqiNP_286002.1. NC_002655.2.
NP_308342.1. NC_002695.1.

3D structure databases

ProteinModelPortaliQ8X6J0.
SMRiQ8X6J0. Positions 1-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z0351.

Proteomic databases

PRIDEiQ8X6J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54610; AAG54610; Z0351.
BAB33738; BAB33738; BAB33738.
GeneIDi914414.
957139.
KEGGiece:Z0351.
ecs:ECs0315.
PATRICi18349572. VBIEscCol44059_0308.

Phylogenomic databases

eggNOGiCOG1319.
HOGENOMiHOG000244727.
KOiK11178.
OMAiIGGFSRQ.
OrthoDBiEOG66TG3V.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-315-MONOMER.
ECOO157:YAGS-MONOMER.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiYAGS_ECO57
AccessioniPrimary (citable) accession number: Q8X6J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.