Q8X6C6 (BETA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline dehydrogenase
Short name=CDH
Short name=CHD
EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Z0398, ECs0357

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00750.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	choline dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Choline dehydrogenase HAMAP MF_00750		PRO_0000205587
Regions
	Nucleotide binding	4 – 33	30	FAD Potential
Sites
	Active site	473	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8X6C6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7DC9B36EC06D24ED	FASTA	562	62,633
	10 20 30 40 50 60 MQFDYIIIGA GSAGNVLATR LTEDPNTSVL LLEAGGPDYR FDFRTQMPAA LAFPLQGKRY 70 80 90 100 110 120 NWAYETEPEP FMNNRRMECG RGKGLGGSSL INGMCYIRGN AMDLDNWAKE PGLENWSYLD 130 140 150 160 170 180 CLPYYRKAET RDVGENDYHG GDGPVSVTTS KPGVNPLFEA MIEAGVQAGY PRTDDLNGYQ 190 200 210 220 230 240 QEGFGPMDRT VTPQGRRAST ARGYLDQAKS RPNLTIRTHA MTDHIIFDCK RAVGVEWLEG 250 260 270 280 290 300 DSTIPTRATA NKEVLLCAGA IASPQILQRS GVGNAELLAE FDIPLVHDLP GVGENLQDHL 310 320 330 340 350 360 EMYLQYECKE PVSLYPALQW WNQPKIGAEW LFGGTGVGAS NHFEAGGFIR SREEFAWPNI 370 380 390 400 410 420 QYHFLPVAIN YNGSNAVKEH GFQCHVGSMR SPSRGHVRIK SRDPHQHPAI LFNYMSHEQD 430 440 450 460 470 480 WQEFRDAIRI TREIMHQPAL DQYRGREISP GTECQTDEQL DEFVRNHAET AFHPCGTCKM 490 500 510 520 530 540 GYDEMSVVDG EGRVHGLEGL RVVDASIMPQ IITGNLNATT IMIGEKMADM IRGKEALPRS 550 560 TAGYFVANGM PVRAKKMSRD LN

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54653.1. BA000007 Genomic DNA. Translation: BAB33780.1.
PIR	A85524. E90673.
RefSeq	NP_286045.1. NC_002655.2. NP_308384.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8X6C6.
SMR	Q8X6C6. Positions 1-531.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000024910; EBESCP00000023803; EBESCG00000023964. EBESCT00000057346; EBESCP00000055174; EBESCG00000056394.
GeneID	914458. 957211.
GenomeReviews	Gene locus Z0398 in contig AE005174_GR. Gene locus ECs0357 in contig BA000007_GR.
KEGG	ece:Z0398. ecs:ECs0357.
PATRIC	18349652. VBIEscCol44059_0348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010954.
HOGENOM	HBG734713.
OMA	RSTAPYY.
ProtClustDB	PRK02106.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0357-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
KO	K00108.
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. BetA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_ECO57

Accession

Primary (citable) accession number: Q8X6C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program