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Protein

Xanthine dehydrogenase FAD-binding subunit

Gene

xdhB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism) (By similarity).By similarity

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

FADBy similarity

Pathwayi: hypoxanthine degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Xanthine dehydrogenase iron-sulfur-binding subunit (xdhC), Xanthine dehydrogenase FAD-binding subunit (xdhB), Xanthine dehydrogenase molybdenum-binding subunit (xdhA)
  2. Xanthine dehydrogenase FAD-binding subunit (xdhB), Xanthine dehydrogenase molybdenum-binding subunit (xdhA)
This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei184FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34FADBy similarity8
Nucleotide bindingi109 – 113FADBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism, Purine salvage
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

UniPathwayiUPA00604; UER00661
UPA00604; UER00662

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase FAD-binding subunit (EC:1.17.1.4)
Gene namesi
Name:xdhB
Ordered Locus Names:Z4206, ECs3740
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660931 – 292Xanthine dehydrogenase FAD-binding subunitAdd BLAST292

Interactioni

Subunit structurei

Heterotrimer of XdhA, XdhB and XdhC.Curated

Protein-protein interaction databases

STRINGi155864.Z4206

Structurei

3D structure databases

ProteinModelPortaliQ8X6C5
SMRiQ8X6C5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 176FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST176

Phylogenomic databases

eggNOGiENOG4108HR6 Bacteria
COG1319 LUCA
HOGENOMiHOG000244729
KOiK13479
OMAiMVEINFD

Family and domain databases

InterProiView protein in InterPro
IPR005107 CO_DH_flav_C
IPR036683 CO_DH_flav_C_dom_sf
IPR016166 FAD-bd_2
IPR036318 FAD-bd_2-like_sf
IPR002346 Mopterin_DH_FAD-bd
PfamiView protein in Pfam
PF03450 CO_deh_flav_C, 1 hit
PF00941 FAD_binding_5, 1 hit
SMARTiView protein in SMART
SM01092 CO_deh_flav_C, 1 hit
SUPFAMiSSF55447 SSF55447, 1 hit
SSF56176 SSF56176, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

Q8X6C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDFASYHRA ATLADAINLL ADNPQAKLLA GGTDVLIQLH HHNDRYRHIV
60 70 80 90 100
DIHNLAELRG ITLAEDGSLR IGSATTFTQL IEDSITQRHL PALCAAASSI
110 120 130 140 150
AGPQIRNVAT YGGNICNGAT SADSATPTLI YDAKLEIHSP RGVRFVPING
160 170 180 190 200
FHTGPGKVSL EHDEILVAFH FPPQPKEHVG SAHFKYAMRD AMDISTIGCA
210 220 230 240 250
AHCRLDNGNF SELRLAFGVA APTPIRCQHA EQTAQNAPLN LQTLEAISES
260 270 280 290
VLQDVAPRSS WRASKEFRLH LIQTMTKKVI SEAVAAAGGK LQ
Length:292
Mass (Da):31,561
Last modified:March 1, 2002 - v1
Checksum:iFEC44F9990BF9BC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG57996.2
BA000007 Genomic DNA Translation: BAB37163.1
PIRiD91096
H85941
RefSeqiNP_311767.1, NC_002695.1
WP_000459192.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG57996; AAG57996; Z4206
BAB37163; BAB37163; BAB37163
GeneIDi916440
KEGGiece:Z4206
ecs:ECs3740
PATRICifig|386585.9.peg.3902

Entry informationi

Entry nameiXDHB_ECO57
AccessioniPrimary (citable) accession number: Q8X6C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health