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Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the stereospecific dehydration of (2S,3S)-2-methylcitrate (2-MC) to yield the cis isomer of 2-methyl-aconitate. Could also catalyze the dehydration of citrate and the hydration of cis-aconitate.By similarity

Catalytic activityi

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity
Citrate = isocitrate.By similarity

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA), Citrate synthase (ECs0745)
  2. 2-methylcitrate dehydratase (prpD), Aconitate hydratase B (ECs0122)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718
UPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
2-methylcitrate dehydrataseBy similarity (EC:4.2.1.79By similarity)
Short name:
2-MC dehydrataseBy similarity
Alternative name(s):
(2S,3S)-2-methylcitrate dehydrataseBy similarity
Probable aconitate hydrataseBy similarity (EC:4.2.1.3By similarity)
Short name:
ACNBy similarity
Short name:
AconitaseBy similarity
Gene namesi
Name:prpD
Ordered Locus Names:Z0429, ECs0387
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002150242 – 4832-methylcitrate dehydrataseAdd BLAST482

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi155864.Z0429

Structurei

3D structure databases

ProteinModelPortaliQ8X693
SMRiQ8X693
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PrpD family.Curated

Phylogenomic databases

eggNOGiENOG4105DXD Bacteria
COG2079 LUCA
HOGENOMiHOG000159916
KOiK01720
OMAiECTKHLG

Family and domain databases

InterProiView protein in InterPro
IPR012705 2Me_IsoCit_deHydtase_PrpD
IPR036148 MmgE/PrpD_sf
IPR005656 MmgE_PrpD
PANTHERiPTHR16943 PTHR16943, 1 hit
PfamiView protein in Pfam
PF03972 MmgE_PrpD, 1 hit
SUPFAMiSSF103378 SSF103378, 1 hit
TIGRFAMsiTIGR02330 prpD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8X693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQINNIRP EFDREIVDIV DYVMNYEISS KVAYDTAHYC LLDTLGCGLE
60 70 80 90 100
ALEYPACKKL LGPIVPGTVV PNGVRVPGTQ FQLDPVQAAF NIGAMIRWLD
110 120 130 140 150
FNDTWLAAEW GHPSDNLGGI LATADWLSRN AVASGKAPLT MKQVLTGMIK
160 170 180 190 200
AHEIQGCIAL ENSFNRVGLD HVLLVKVAST AVVAEMLGLT REEILNAVSL
210 220 230 240 250
AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK TGEMGYPSAL
260 270 280 290 300
TAPVWGFYDV SFKGESFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA
310 320 330 340 350
MTLYEQMQAA GKTAADIEKV SIRTHEACIR IIDKKGPLNN PADRDHCIQY
360 370 380 390 400
MVAIPLLFGR LTAADYEDNV AQDKRIDALR EKINCFEDPV FTADYHDPEK
410 420 430 440 450
RAIANAITLE FTDGTRFEEV VVEYPIGHAR RRQDGIPKLV DKFKINLARQ
460 470 480
FPTRQQQRIL EVSLDRARLE QMPVNEYLDL YVI
Length:483
Mass (Da):53,952
Last modified:January 23, 2007 - v3
Checksum:i288942C490E56F86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG54682.1
BA000007 Genomic DNA Translation: BAB33810.1
PIRiC90677
F85527
RefSeqiNP_308414.1, NC_002695.1
WP_001275870.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG54682; AAG54682; Z0429
BAB33810; BAB33810; BAB33810
GeneIDi914489
KEGGiece:Z0429
ecs:ECs0387
PATRICifig|386585.9.peg.482

Similar proteinsi

Entry informationi

Entry nameiPRPD_ECO57
AccessioniPrimary (citable) accession number: Q8X693
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 98 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health