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Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the stereospecific dehydration of (2S,3S)-2-methylcitrate (2-MC) to yield the cis isomer of 2-methyl-aconitate. Could also catalyze the dehydration of citrate and the hydration of cis-aconitate.By similarity

Catalytic activityi

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity
Citrate = isocitrate.By similarity

Pathway:ipropanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Pathway:itricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. 2-methylcitrate dehydratase (prpD), Aconitate hydratase B (acnB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciECOL386585:GJFA-381-MONOMER.
ECOO157:PRPD-MONOMER.
UniPathwayiUPA00223; UER00718.
UPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
2-methylcitrate dehydrataseBy similarity (EC:4.2.1.79By similarity)
Short name:
2-MC dehydrataseBy similarity
Alternative name(s):
(2S,3S)-2-methylcitrate dehydrataseBy similarity
Probable aconitate hydrataseBy similarity (EC:4.2.1.3By similarity)
Short name:
ACNBy similarity
Short name:
AconitaseBy similarity
Gene namesi
Name:prpD
Ordered Locus Names:Z0429, ECs0387
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558 Componenti: Chromosome UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 4834822-methylcitrate dehydratasePRO_0000215024Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi155864.Z0429.

Structurei

3D structure databases

ProteinModelPortaliQ8X693.
SMRiQ8X693. Positions 11-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PrpD family.Curated

Phylogenomic databases

eggNOGiCOG2079.
HOGENOMiHOG000159916.
KOiK01720.
OMAiPECTKHL.
OrthoDBiEOG6RC3K5.

Family and domain databases

InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERiPTHR16943. PTHR16943. 1 hit.
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.
TIGRFAMsiTIGR02330. prpD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8X693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQINNIRP EFDREIVDIV DYVMNYEISS KVAYDTAHYC LLDTLGCGLE
60 70 80 90 100
ALEYPACKKL LGPIVPGTVV PNGVRVPGTQ FQLDPVQAAF NIGAMIRWLD
110 120 130 140 150
FNDTWLAAEW GHPSDNLGGI LATADWLSRN AVASGKAPLT MKQVLTGMIK
160 170 180 190 200
AHEIQGCIAL ENSFNRVGLD HVLLVKVAST AVVAEMLGLT REEILNAVSL
210 220 230 240 250
AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK TGEMGYPSAL
260 270 280 290 300
TAPVWGFYDV SFKGESFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA
310 320 330 340 350
MTLYEQMQAA GKTAADIEKV SIRTHEACIR IIDKKGPLNN PADRDHCIQY
360 370 380 390 400
MVAIPLLFGR LTAADYEDNV AQDKRIDALR EKINCFEDPV FTADYHDPEK
410 420 430 440 450
RAIANAITLE FTDGTRFEEV VVEYPIGHAR RRQDGIPKLV DKFKINLARQ
460 470 480
FPTRQQQRIL EVSLDRARLE QMPVNEYLDL YVI
Length:483
Mass (Da):53,952
Last modified:January 23, 2007 - v3
Checksum:i288942C490E56F86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54682.1.
BA000007 Genomic DNA. Translation: BAB33810.1.
PIRiC90677.
F85527.
RefSeqiNP_308414.1. NC_002695.1.
WP_001275870.1. NZ_LAZD01000108.1.

Genome annotation databases

EnsemblBacteriaiAAG54682; AAG54682; Z0429.
BAB33810; BAB33810; BAB33810.
GeneIDi914489.
KEGGiecs:ECs0387.
PATRICi18349721. VBIEscCol44059_0381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54682.1.
BA000007 Genomic DNA. Translation: BAB33810.1.
PIRiC90677.
F85527.
RefSeqiNP_308414.1. NC_002695.1.
WP_001275870.1. NZ_LAZD01000108.1.

3D structure databases

ProteinModelPortaliQ8X693.
SMRiQ8X693. Positions 11-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z0429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54682; AAG54682; Z0429.
BAB33810; BAB33810; BAB33810.
GeneIDi914489.
KEGGiecs:ECs0387.
PATRICi18349721. VBIEscCol44059_0381.

Phylogenomic databases

eggNOGiCOG2079.
HOGENOMiHOG000159916.
KOiK01720.
OMAiPECTKHL.
OrthoDBiEOG6RC3K5.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
UPA00946.
BioCyciECOL386585:GJFA-381-MONOMER.
ECOO157:PRPD-MONOMER.

Family and domain databases

InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERiPTHR16943. PTHR16943. 1 hit.
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.
TIGRFAMsiTIGR02330. prpD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPRPD_ECO57
AccessioniPrimary (citable) accession number: Q8X693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.