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Q8X693 (PRPD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-methylcitrate dehydratase

EC=4.2.1.79
Gene names
Name:prpD
Ordered Locus Names:Z0429, ECs0387
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.

Catalytic activity

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

Cofactor

Binds 1 2Fe-2S cluster By similarity.

Pathway

Organic acid metabolism; propanoate degradation.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the PrpD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 4834822-methylcitrate dehydratase
PRO_0000215024

Sequences

Sequence LengthMass (Da)Tools
Q8X693 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 288942C490E56F86

FASTA48353,952
        10         20         30         40         50         60 
MSAQINNIRP EFDREIVDIV DYVMNYEISS KVAYDTAHYC LLDTLGCGLE ALEYPACKKL 

        70         80         90        100        110        120 
LGPIVPGTVV PNGVRVPGTQ FQLDPVQAAF NIGAMIRWLD FNDTWLAAEW GHPSDNLGGI 

       130        140        150        160        170        180 
LATADWLSRN AVASGKAPLT MKQVLTGMIK AHEIQGCIAL ENSFNRVGLD HVLLVKVAST 

       190        200        210        220        230        240 
AVVAEMLGLT REEILNAVSL AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK 

       250        260        270        280        290        300 
TGEMGYPSAL TAPVWGFYDV SFKGESFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA 

       310        320        330        340        350        360 
MTLYEQMQAA GKTAADIEKV SIRTHEACIR IIDKKGPLNN PADRDHCIQY MVAIPLLFGR 

       370        380        390        400        410        420 
LTAADYEDNV AQDKRIDALR EKINCFEDPV FTADYHDPEK RAIANAITLE FTDGTRFEEV 

       430        440        450        460        470        480 
VVEYPIGHAR RRQDGIPKLV DKFKINLARQ FPTRQQQRIL EVSLDRARLE QMPVNEYLDL 


YVI 

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG54682.1.
BA000007 Genomic DNA. Translation: BAB33810.1.
PIRC90677.
F85527.
RefSeqNP_286074.1. NC_002655.2.
NP_308414.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8X693.
SMRQ8X693. Positions 11-483.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z0429.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG54682; AAG54682; Z0429.
BAB33810; BAB33810; BAB33810.
GeneID914489.
957258.
KEGGece:Z0429.
ecs:ECs0387.
PATRIC18349721. VBIEscCol44059_0381.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2079.
HOGENOMHOG000159916.
KOK01720.
OMAPNAGSRK.
OrthoDBEOG6RC3K5.
ProtClustDBPRK09425.

Enzyme and pathway databases

BioCycECOL386585:GJFA-381-MONOMER.
ECOO157:PRPD-MONOMER.
UniPathwayUPA00946.

Family and domain databases

InterProIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERPTHR16943. PTHR16943. 1 hit.
PfamPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMSSF103378. SSF103378. 1 hit.
TIGRFAMsTIGR02330. prpD. 1 hit.
ProtoNetSearch...

Entry information

Entry namePRPD_ECO57
AccessionPrimary (citable) accession number: Q8X693
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: January 23, 2007
Last modified: December 11, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways