Q8X693 (PRPD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-methylcitrate dehydratase
EC=4.2.1.79

Gene names

Name:	prpD
Ordered Locus Names:	Z0429, ECs0387

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.
Catalytic activity	(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.
Cofactor	Binds 1 2Fe-2S cluster By similarity.
Pathway	Organic acid metabolism; propanoate degradation.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the prpD family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 2-methylcitrate dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 483	482	2-methylcitrate dehydratase		PRO_0000215024

Sequences

	Sequence		Length	Mass (Da)
	Q8X693 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 288942C490E56F86	FASTA	483	53,952
	10 20 30 40 50 60 MSAQINNIRP EFDREIVDIV DYVMNYEISS KVAYDTAHYC LLDTLGCGLE ALEYPACKKL 70 80 90 100 110 120 LGPIVPGTVV PNGVRVPGTQ FQLDPVQAAF NIGAMIRWLD FNDTWLAAEW GHPSDNLGGI 130 140 150 160 170 180 LATADWLSRN AVASGKAPLT MKQVLTGMIK AHEIQGCIAL ENSFNRVGLD HVLLVKVAST 190 200 210 220 230 240 AVVAEMLGLT REEILNAVSL AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK 250 260 270 280 290 300 TGEMGYPSAL TAPVWGFYDV SFKGESFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA 310 320 330 340 350 360 MTLYEQMQAA GKTAADIEKV SIRTHEACIR IIDKKGPLNN PADRDHCIQY MVAIPLLFGR 370 380 390 400 410 420 LTAADYEDNV AQDKRIDALR EKINCFEDPV FTADYHDPEK RAIANAITLE FTDGTRFEEV 430 440 450 460 470 480 VVEYPIGHAR RRQDGIPKLV DKFKINLARQ FPTRQQQRIL EVSLDRARLE QMPVNEYLDL YVI

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54682.1. BA000007 Genomic DNA. Translation: BAB33810.1.
PIR	C90677. F85527.
RefSeq	NP_286074.1. NC_002655.2. NP_308414.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8X693.
SMR	Q8X693. Positions 11-483.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000025526; EBESCP00000024419; EBESCG00000024579. EBESCT00000058185; EBESCP00000056013; EBESCG00000057233.
GeneID	914489. 957258.
GenomeReviews	Gene locus Z0429 in contig AE005174_GR. Gene locus ECs0387 in contig BA000007_GR.
KEGG	ece:Z0429. ecs:ECs0387.
PATRIC	18349721. VBIEscCol44059_0381.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000011071.
HOGENOM	HBG298383.
OMA	CLMDTLG.
ProtClustDB	PRK09425.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0387-MONOMER.
Family and domain databases
InterPro	IPR012705. 2Me_IsoCit_deHydtase_PrpD. IPR005656. MmgE_PrpD. [Graphical view]
KO	K01720.
PANTHER	PTHR16943. MmgE_PrpD. 1 hit.
Pfam	PF03972. MmgE_PrpD. 1 hit. [Graphical view]
SUPFAM	SSF103378. MmgE_PrpD. 1 hit.
TIGRFAMs	TIGR02330. PrpD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRPD_ECO57

Accession

Primary (citable) accession number: Q8X693

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program