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Q8X612 (PUR2_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Z5582, ECs4928
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138

Subunit structure

Monomer By similarity. HAMAP MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Phosphoribosylamine--glycine ligase HAMAP MF_00138
PRO_0000151450

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8X612 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: 5F8D9E5961330672

FASTA42946,035
        10         20         30         40         50         60 
MKVLVIGNGG REHALAWKAA QSPLVETVFV APGNAGTALE PTLQNVAIGV TDIPALLDFA 

        70         80         90        100        110        120 
QNEKVDLTIV GPEAPLVKGV VDTFRAAGMK IFGPTAGAAQ LEGSKAFTKD FLARHNIPTA 

       130        140        150        160        170        180 
EYQNFTEVEP ALAYLREKGA PIVIKADGLA AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA 

       190        200        210        220        230        240 
GHRIVIEEFL DGEEASFIVM VDGEHVLPMA TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT 

       250        260        270        280        290        300 
DDVHQRTMER IIWPTVKGMA SEGNTYTGFL YAGLMIDKQG NPKVIEFNCR FGDPETQPIM 

       310        320        330        340        350        360 
LRMKSDLVEL CLAACEGKLD EKTSEWDERA SLGVVMAAGG YPGDYRTGDV IHGLPLEEVE 

       370        380        390        400        410        420 
DGKVFHAGTK LADDEQVVTS GGRVLCVTAL GHTVAEAQKR AYALMTDIHW DDCFCRKDIG 


WRAIEREQN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG59202.1.
BA000007 Genomic DNA. Translation: BAB38351.1.
PIRF86092.
H91244.
RefSeqNP_290637.1. NC_002655.2.
NP_312955.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8X612.
SMRQ8X612. Positions 1-426.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000025429; EBESCP00000024322; EBESCG00000024483.
EBESCT00000058616; EBESCP00000056444; EBESCG00000057664.
GeneID914922.
960124.
GenomeReviewsGene locus Z5582 in contig AE005174_GR.
Gene locus ECs4928 in contig BA000007_GR.
KEGGece:Z5582.
ecs:ECs4928.
PATRIC18359551. VBIEscCol44059_4916.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000010610.
HOGENOMHBG404060.
OMAMGAYTPL.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycECOL83334:ECS4928-MONOMER.

Family and domain databases

HAMAPMF_00138. GARS.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.90.600.10. Gars. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01945.
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR00877. PurD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_ECO57
AccessionPrimary (citable) accession number: Q8X612
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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