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Protein

Isocitrate dehydrogenase kinase/phosphatase

Gene

aceK

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.UniRule annotation

Catalytic activityi

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei336ATPUniRule annotation1
Active sitei371UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi315 – 321ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOO157:ACEK-MONOMER.
BRENDAi2.7.11.5. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation)
Short name:
IDH kinase/phosphataseUniRule annotation
Short name:
IDHK/PUniRule annotation
Gene namesi
Name:aceKUniRule annotation
Ordered Locus Names:Z5602, ECs4934
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579001 – 578Isocitrate dehydrogenase kinase/phosphataseAdd BLAST578

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5602.

Structurei

Secondary structure

1578
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 28Combined sources26
Helixi31 – 36Combined sources6
Helixi40 – 67Combined sources28
Turni71 – 74Combined sources4
Helixi76 – 87Combined sources12
Turni88 – 92Combined sources5
Helixi96 – 110Combined sources15
Turni111 – 113Combined sources3
Turni118 – 120Combined sources3
Beta strandi139 – 143Combined sources5
Beta strandi149 – 151Combined sources3
Helixi152 – 157Combined sources6
Beta strandi164 – 167Combined sources4
Helixi169 – 184Combined sources16
Helixi186 – 189Combined sources4
Beta strandi193 – 199Combined sources7
Beta strandi201 – 203Combined sources3
Beta strandi206 – 215Combined sources10
Beta strandi218 – 228Combined sources11
Beta strandi232 – 236Combined sources5
Helixi243 – 249Combined sources7
Helixi264 – 273Combined sources10
Helixi280 – 287Combined sources8
Helixi290 – 306Combined sources17
Beta strandi312 – 314Combined sources3
Beta strandi316 – 318Combined sources3
Beta strandi321 – 327Combined sources7
Beta strandi332 – 338Combined sources7
Helixi350 – 361Combined sources12
Turni366 – 368Combined sources3
Beta strandi373 – 381Combined sources9
Helixi382 – 384Combined sources3
Helixi387 – 396Combined sources10
Helixi398 – 400Combined sources3
Beta strandi401 – 404Combined sources4
Beta strandi407 – 417Combined sources11
Helixi422 – 428Combined sources7
Helixi432 – 450Combined sources19
Helixi460 – 462Combined sources3
Beta strandi463 – 465Combined sources3
Beta strandi471 – 473Combined sources3
Helixi476 – 478Combined sources3
Beta strandi480 – 482Combined sources3
Helixi496 – 500Combined sources5
Helixi517 – 522Combined sources6
Turni526 – 528Combined sources3
Helixi529 – 535Combined sources7
Helixi537 – 540Combined sources4
Helixi542 – 553Combined sources12
Helixi565 – 567Combined sources3
Helixi569 – 572Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EPSX-ray2.80A/B2-578[»]
3LC6X-ray3.10A/B1-578[»]
3LCBX-ray2.90A/B1-578[»]
4P69X-ray3.30A/B4-571[»]
SMRiQ8X607.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8X607.

Family & Domainsi

Sequence similaritiesi

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105VS7. Bacteria.
COG4579. LUCA.
HOGENOMiHOG000247673.
KOiK00906.
OMAiEPWYSVG.

Family and domain databases

HAMAPiMF_00747. AceK. 1 hit.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q8X607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR
60 70 80 90 100
IHLYDHHVGL VVEQLRCITN GQSTDAEFLL RVKEHYTRLL PDYPRFEIAE
110 120 130 140 150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE
160 170 180 190 200
SLLMRVISDL PLRLHWQNKS RDIHYIIRHL TETLGPENLS KSHLQVANEL
210 220 230 240 250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF
260 270 280 290 300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
310 320 330 340 350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD KFAPQKEMSA
360 370 380 390 400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK
410 420 430 440 450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA
460 470 480 490 500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL
510 520 530 540 550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN
560 570
RIREGHVEDV YAYRRRQRFS VRYGEMLF
Length:578
Mass (Da):67,735
Last modified:March 1, 2002 - v1
Checksum:iB6C1AF54D272685A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59208.1.
BA000007 Genomic DNA. Translation: BAB38357.1.
PIRiD86093.
F91245.
RefSeqiNP_312961.1. NC_002695.1.
WP_001137245.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG59208; AAG59208; Z5602.
BAB38357; BAB38357; BAB38357.
GeneIDi914849.
KEGGiece:Z5602.
ecs:ECs4934.
PATRICi18359573. VBIEscCol44059_4923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59208.1.
BA000007 Genomic DNA. Translation: BAB38357.1.
PIRiD86093.
F91245.
RefSeqiNP_312961.1. NC_002695.1.
WP_001137245.1. NZ_LPWC02000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EPSX-ray2.80A/B2-578[»]
3LC6X-ray3.10A/B1-578[»]
3LCBX-ray2.90A/B1-578[»]
4P69X-ray3.30A/B4-571[»]
SMRiQ8X607.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG59208; AAG59208; Z5602.
BAB38357; BAB38357; BAB38357.
GeneIDi914849.
KEGGiece:Z5602.
ecs:ECs4934.
PATRICi18359573. VBIEscCol44059_4923.

Phylogenomic databases

eggNOGiENOG4105VS7. Bacteria.
COG4579. LUCA.
HOGENOMiHOG000247673.
KOiK00906.
OMAiEPWYSVG.

Enzyme and pathway databases

BioCyciECOO157:ACEK-MONOMER.
BRENDAi2.7.11.5. 2026.

Miscellaneous databases

EvolutionaryTraceiQ8X607.

Family and domain databases

HAMAPiMF_00747. AceK. 1 hit.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiACEK_ECO57
AccessioniPrimary (citable) accession number: Q8X607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.