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Protein

Isocitrate dehydrogenase kinase/phosphatase

Gene

aceK

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.UniRule annotation

Catalytic activityi

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei336 – 3361ATPUniRule annotation
Active sitei371 – 3711UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi315 – 3217ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4938-MONOMER.
ECOO157:ACEK-MONOMER.
BRENDAi2.7.11.5. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation)
Short name:
IDH kinase/phosphataseUniRule annotation
Short name:
IDHK/PUniRule annotation
Gene namesi
Name:aceKUniRule annotation
Ordered Locus Names:Z5602, ECs4934
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Isocitrate dehydrogenase kinase/phosphatasePRO_0000057900Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5602.

Structurei

Secondary structure

1
578
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2826Combined sources
Helixi31 – 366Combined sources
Helixi40 – 6728Combined sources
Turni71 – 744Combined sources
Helixi76 – 8712Combined sources
Turni88 – 925Combined sources
Helixi96 – 11015Combined sources
Turni111 – 1133Combined sources
Turni118 – 1203Combined sources
Beta strandi139 – 1435Combined sources
Beta strandi149 – 1513Combined sources
Helixi152 – 1576Combined sources
Beta strandi164 – 1674Combined sources
Helixi169 – 18416Combined sources
Helixi186 – 1894Combined sources
Beta strandi193 – 1997Combined sources
Beta strandi201 – 2033Combined sources
Beta strandi206 – 21510Combined sources
Beta strandi218 – 22811Combined sources
Beta strandi232 – 2365Combined sources
Helixi243 – 2497Combined sources
Helixi264 – 27310Combined sources
Helixi280 – 2878Combined sources
Helixi290 – 30617Combined sources
Beta strandi312 – 3143Combined sources
Beta strandi316 – 3183Combined sources
Beta strandi321 – 3277Combined sources
Beta strandi332 – 3387Combined sources
Helixi350 – 36112Combined sources
Turni366 – 3683Combined sources
Beta strandi373 – 3819Combined sources
Helixi382 – 3843Combined sources
Helixi387 – 39610Combined sources
Helixi398 – 4003Combined sources
Beta strandi401 – 4044Combined sources
Beta strandi407 – 41711Combined sources
Helixi422 – 4287Combined sources
Helixi432 – 45019Combined sources
Helixi460 – 4623Combined sources
Beta strandi463 – 4653Combined sources
Beta strandi471 – 4733Combined sources
Helixi476 – 4783Combined sources
Beta strandi480 – 4823Combined sources
Helixi496 – 5005Combined sources
Helixi517 – 5226Combined sources
Turni526 – 5283Combined sources
Helixi529 – 5357Combined sources
Helixi537 – 5404Combined sources
Helixi542 – 55312Combined sources
Helixi565 – 5673Combined sources
Helixi569 – 5724Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EPSX-ray2.80A/B2-578[»]
3LC6X-ray3.10A/B1-578[»]
3LCBX-ray2.90A/B1-578[»]
4P69X-ray3.30A/B4-571[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8X607.

Family & Domainsi

Sequence similaritiesi

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105VS7. Bacteria.
COG4579. LUCA.
HOGENOMiHOG000247673.
KOiK00906.
OMAiEPWYSVG.

Family and domain databases

HAMAPiMF_00747. AceK. 1 hit.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q8X607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR
60 70 80 90 100
IHLYDHHVGL VVEQLRCITN GQSTDAEFLL RVKEHYTRLL PDYPRFEIAE
110 120 130 140 150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE
160 170 180 190 200
SLLMRVISDL PLRLHWQNKS RDIHYIIRHL TETLGPENLS KSHLQVANEL
210 220 230 240 250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF
260 270 280 290 300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
310 320 330 340 350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD KFAPQKEMSA
360 370 380 390 400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK
410 420 430 440 450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA
460 470 480 490 500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL
510 520 530 540 550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN
560 570
RIREGHVEDV YAYRRRQRFS VRYGEMLF
Length:578
Mass (Da):67,735
Last modified:March 1, 2002 - v1
Checksum:iB6C1AF54D272685A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59208.1.
BA000007 Genomic DNA. Translation: BAB38357.1.
PIRiD86093.
F91245.
RefSeqiNP_312961.1. NC_002695.1.
WP_001137245.1. NZ_LPWC01000346.1.

Genome annotation databases

EnsemblBacteriaiAAG59208; AAG59208; Z5602.
BAB38357; BAB38357; BAB38357.
GeneIDi914849.
KEGGiece:Z5602.
ecs:ECs4934.
PATRICi18359573. VBIEscCol44059_4923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59208.1.
BA000007 Genomic DNA. Translation: BAB38357.1.
PIRiD86093.
F91245.
RefSeqiNP_312961.1. NC_002695.1.
WP_001137245.1. NZ_LPWC01000346.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EPSX-ray2.80A/B2-578[»]
3LC6X-ray3.10A/B1-578[»]
3LCBX-ray2.90A/B1-578[»]
4P69X-ray3.30A/B4-571[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG59208; AAG59208; Z5602.
BAB38357; BAB38357; BAB38357.
GeneIDi914849.
KEGGiece:Z5602.
ecs:ECs4934.
PATRICi18359573. VBIEscCol44059_4923.

Phylogenomic databases

eggNOGiENOG4105VS7. Bacteria.
COG4579. LUCA.
HOGENOMiHOG000247673.
KOiK00906.
OMAiEPWYSVG.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4938-MONOMER.
ECOO157:ACEK-MONOMER.
BRENDAi2.7.11.5. 2026.

Miscellaneous databases

EvolutionaryTraceiQ8X607.

Family and domain databases

HAMAPiMF_00747. AceK. 1 hit.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiACEK_ECO57
AccessioniPrimary (citable) accession number: Q8X607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.