Q8X5L9 (GUN_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase
Short name=CMCase
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:	bcsZ
Ordered Locus Names:	Z4946, ECs4411

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes carboxymethylcellulose By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 368	347	Endoglucanase		PRO_0000007939
Sites
	Active site	55	1	Proton donor By similarity
	Active site	116	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8X5L9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: ECFD4234AD5113D2	FASTA	368	41,760
	10 20 30 40 50 60 MNVLRSGLVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK ITTSEGQSYG 70 80 90 100 110 120 MFFALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK KENSKWEVLD SNSASDGDVW 130 140 150 160 170 180 MAWSLLEAGR LWKEQRYTDI GSALLKRIAR EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP 190 200 210 220 230 240 SYLPPTLAQY FTRFGAPWTT LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS 250 260 270 280 290 300 SYDAIRVYMW VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF 310 320 330 340 350 360 SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF STKGELLPDW GQECANSH

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58673.1. BA000007 Genomic DNA. Translation: BAB37834.1.
PIR	C91180. E86026.
RefSeq	NP_290111.1. NC_002655.2. NP_312438.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8X5L9.
SMR	Q8X5L9. Positions 26-347.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z4946.
Protein family/group databases
CAZy	GH8. Glycoside Hydrolase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG58673; AAG58673; Z4946. BAB37834; BAB37834; BAB37834.
GeneID	915724. 961090.
KEGG	ece:Z4946. ecs:ECs4411.
PATRIC	18358405. VBIEscCol44059_4369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3405.
HOGENOM	HOG000259088.
KO	K01179.
OMA	IRVYLWV.
ProtClustDB	PRK11097.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-4378-MONOMER.
UniPathway	UPA00694.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. IPR019834. Glyco_hydro_8_CS. [Graphical view]
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00812. GLYCOSYL_HYDROL_F8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_ECO57

Accession

Primary (citable) accession number: Q8X5L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program