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Protein

Endoglucanase

Gene

bcsZ

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes carboxymethylcellulose.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55Proton donorBy similarity1
Active sitei116NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00694.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethylcellulase
Short name:
CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:bcsZ
Ordered Locus Names:Z4946, ECs4411
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000793922 – 368EndoglucanaseAdd BLAST347

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4946.

Structurei

3D structure databases

ProteinModelPortaliQ8X5L9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105K2Z. Bacteria.
COG3405. LUCA.
HOGENOMiHOG000259088.
KOiK20542.
OMAiIRVYLWV.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8X5L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLRSGLVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK
60 70 80 90 100
ITTSEGQSYG MFFALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK
110 120 130 140 150
KENSKWEVLD SNSASDGDVW MAWSLLEAGR LWKEQRYTDI GSALLKRIAR
160 170 180 190 200
EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP SYLPPTLAQY FTRFGAPWTT
210 220 230 240 250
LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS SYDAIRVYMW
260 270 280 290 300
VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF
310 320 330 340 350
SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF
360
STKGELLPDW GQECANSH
Length:368
Mass (Da):41,760
Last modified:March 1, 2002 - v1
Checksum:iECFD4234AD5113D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58673.1.
BA000007 Genomic DNA. Translation: BAB37834.1.
PIRiC91180.
E86026.
RefSeqiNP_312438.1. NC_002695.1.
WP_001302749.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG58673; AAG58673; Z4946.
BAB37834; BAB37834; BAB37834.
GeneIDi915724.
KEGGiece:Z4946.
ecs:ECs4411.
PATRICi18358405. VBIEscCol44059_4369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58673.1.
BA000007 Genomic DNA. Translation: BAB37834.1.
PIRiC91180.
E86026.
RefSeqiNP_312438.1. NC_002695.1.
WP_001302749.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliQ8X5L9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z4946.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58673; AAG58673; Z4946.
BAB37834; BAB37834; BAB37834.
GeneIDi915724.
KEGGiece:Z4946.
ecs:ECs4411.
PATRICi18358405. VBIEscCol44059_4369.

Phylogenomic databases

eggNOGiENOG4105K2Z. Bacteria.
COG3405. LUCA.
HOGENOMiHOG000259088.
KOiK20542.
OMAiIRVYLWV.

Enzyme and pathway databases

UniPathwayiUPA00694.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN_ECO57
AccessioniPrimary (citable) accession number: Q8X5L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.