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Q8X5J4 (FRMA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
S-(hydroxymethyl)glutathione dehydrogenase
EC=1.1.1.284
Alternative name(s):
Alcohol dehydrogenase class-3
EC=1.1.1.1
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase
Short name=FALDH
Short name=FDH
Short name=GSH-FDH
EC=1.1.1.-
Gene names
Name:frmA
Ordered Locus Names:Z0456, ECs0411
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent By similarity.

Catalytic activity

S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369S-(hydroxymethyl)glutathione dehydrogenase
PRO_0000341287

Sites

Metal binding401Zinc 1; catalytic By similarity
Metal binding621Zinc 1; catalytic By similarity
Metal binding921Zinc 2 By similarity
Metal binding951Zinc 2 By similarity
Metal binding981Zinc 2 By similarity
Metal binding1061Zinc 2 By similarity
Metal binding1691Zinc 1; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8X5J4 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 37C601848801ECFB

FASTA36939,333
        10         20         30         40         50         60 
MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD DPEGVFPVVL 

        70         80         90        100        110        120 
GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS GKTNLCVAVR ETQGKGLMPD 

       130        140        150        160        170        180 
GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV SLAKINPEAN HEHVCLLGCG VTTGIGAVHN 

       190        200        210        220        230        240 
TAKVQPGDSV AVFGLGAIGL AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND 

       250        260        270        280        290        300 
YDKPIKDVLL DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA GSGQEISTRP 

       310        320        330        340        350        360 
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI NDAFDLMHEG 


KSIRTVIRY

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG54707.1.
BA000007 Genomic DNA. Translation: BAB33834.1.
PIRC90680.
G85530.
RefSeqNP_286099.1. NC_002655.2.
NP_308438.1. NC_002695.1.

3D structure databases

HSSPHSSP built from PDB template 1M6H based on UniProtKB P11766.
ProteinModelPortalQ8X5J4.
SMRQ8X5J4. Positions 1-368.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000026595; EBESCP00000025488; EBESCG00000025648.
EBESCT00000057631; EBESCP00000055459; EBESCG00000056679.
GeneID914513.
957296.
GenomeReviewsGene locus Z0456 in contig AE005174_GR.
Gene locus ECs0411 in contig BA000007_GR.
KEGGece:Z0456.
ecs:ECs0411.
PATRIC18349769. VBIEscCol44059_0405.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000008796.
HOGENOMHBG753318.
OMAVDHTFEC.
ProtClustDBCLSK2393163.

Enzyme and pathway databases

BioCycECOL83334:ECS0411-MONOMER.

Family and domain databases

InterProIPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00121.
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMSSF50129. GroES_like. 2 hits.
TIGRFAMsTIGR02818. Adh_III_F_hyde. 1 hit.
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFRMA_ECO57
AccessionPrimary (citable) accession number: Q8X5J4
Secondary accession number(s): Q7AH45
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2002
Last modified: January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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