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Protein

S-(hydroxymethyl)glutathione dehydrogenase

Gene

frmA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent.By similarity

Catalytic activityi

S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Zinc 1; catalyticBy similarity1
Metal bindingi62Zinc 1; catalyticBy similarity1
Metal bindingi92Zinc 2By similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi98Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi169Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Alternative name(s):
Alcohol dehydrogenase class-3 (EC:1.1.1.1)
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
GSH-FDH
Gene namesi
Name:frmA
Ordered Locus Names:Z0456, ECs0411
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003412871 – 369S-(hydroxymethyl)glutathione dehydrogenaseAdd BLAST369

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z0456.

Structurei

3D structure databases

ProteinModelPortaliQ8X5J4.
SMRiQ8X5J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QPD. Bacteria.
COG1062. LUCA.
HOGENOMiHOG000294674.
KOiK00121.
OMAiMITHVLK.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiView protein in PROSITE
PS00059. ADH_ZINC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8X5J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD
60 70 80 90 100
DPEGVFPVVL GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS
110 120 130 140 150
GKTNLCVAVR ETQGKGLMPD GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV
160 170 180 190 200
SLAKINPEAN HEHVCLLGCG VTTGIGAVHN TAKVQPGDSV AVFGLGAIGL
210 220 230 240 250
AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND YDKPIKDVLL
260 270 280 290 300
DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA GSGQEISTRP
310 320 330 340 350
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI
360
NDAFDLMHEG KSIRTVIRY
Length:369
Mass (Da):39,333
Last modified:March 1, 2002 - v1
Checksum:i37C601848801ECFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54707.1.
BA000007 Genomic DNA. Translation: BAB33834.1.
PIRiC90680.
G85530.
RefSeqiNP_308438.1. NC_002695.1.
WP_000842102.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG54707; AAG54707; Z0456.
BAB33834; BAB33834; BAB33834.
GeneIDi914513.
KEGGiece:Z0456.
ecs:ECs0411.
PATRICi18349769. VBIEscCol44059_0405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54707.1.
BA000007 Genomic DNA. Translation: BAB33834.1.
PIRiC90680.
G85530.
RefSeqiNP_308438.1. NC_002695.1.
WP_000842102.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliQ8X5J4.
SMRiQ8X5J4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z0456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54707; AAG54707; Z0456.
BAB33834; BAB33834; BAB33834.
GeneIDi914513.
KEGGiece:Z0456.
ecs:ECs0411.
PATRICi18349769. VBIEscCol44059_0405.

Phylogenomic databases

eggNOGiENOG4107QPD. Bacteria.
COG1062. LUCA.
HOGENOMiHOG000294674.
KOiK00121.
OMAiMITHVLK.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiView protein in PROSITE
PS00059. ADH_ZINC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFRMA_ECO57
AccessioniPrimary (citable) accession number: Q8X5J4
Secondary accession number(s): Q7AH45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2002
Last modified: March 15, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.