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Q8X4P8 (FUMA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase class I, aerobic

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fumA
Ordered Locus Names:Z2615, ECs2318
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically By similarity.

Catalytic activity

(S)-malate = fumarate + H2O.

Cofactor

Binds 1 4Fe-4S cluster By similarity.

Enzyme regulation

Subject to aerobic respiratory control and catabolite repression.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the class-I fumarase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

fumarate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 548547Fumarate hydratase class I, aerobic
PRO_0000195657

Sites

Active site3971 Potential
Metal binding3181Iron-sulfur (4Fe-4S) By similarity
Binding site4631Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q8X4P8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F26C7AA07630563F

FASTA54860,298
        10         20         30         40         50         60 
MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS LSEFEGQEIL KVAPEALTLL ARQAFHDASF 

        70         80         90        100        110        120 
MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG VLPTCQDTGT AIIVGKKGQR 

       130        140        150        160        170        180 
VWTGGGDEAA LARGVYNTYI EDNLRYSQNA PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF 

       190        200        210        220        230        240 
LCIAKGGGSA NKTYLYQETK ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET 

       250        260        270        280        290        300 
NLKTVKLASA KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 

       310        320        330        340        350        360 
IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL RKAGEGEAVR 

       370        380        390        400        410        420 
VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL KERMDNGEGL PQYIKDHPIY 

       430        440        450        460        470        480 
YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ LQALGGSMIM LAKGNRSQQV TDACKKHGGF 

       490        500        510        520        530        540 
YLGSIGGPAA VLAQGSIKSL ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL 


TQCTRCVK 

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG56599.1.
BA000007 Genomic DNA. Translation: BAB35741.1.
PIRC85767.
F90918.
RefSeqNP_288047.1. NC_002655.2.
NP_310345.1. NC_002695.1.

3D structure databases

ProteinModelPortalQ8X4P8.
SMRQ8X4P8. Positions 356-537.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z2615.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG56599; AAG56599; Z2615.
BAB35741; BAB35741; BAB35741.
GeneID916929.
961562.
KEGGece:Z2615.
ecs:ECs2318.
PATRIC18353964. VBIEscCol44059_2195.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1951.
HOGENOMHOG000009338.
KOK01676.
OMATCVEYPE.
OrthoDBEOG6TXR10.

Enzyme and pathway databases

BioCycECOL386585:GJFA-2292-MONOMER.
ECOO157:FUMA-MONOMER.
UniPathwayUPA00223; UER01007.

Family and domain databases

Gene3D3.20.130.10. 1 hit.
InterProIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMSSF117457. SSF117457. 1 hit.
TIGRFAMsTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFUMA_ECO57
AccessionPrimary (citable) accession number: Q8X4P8
Secondary accession number(s): Q7ADM1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways