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Protein

Fumarate hydratase class I, aerobic

Gene

fumA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible hydration of fumarate to (S)-malate. Functions as an aerobic enzyme in the direction of malate formation as part of the citric acid cycle. Accounts for about 80% of the fumarase activity when the bacteria grow aerobically. To a lesser extent, also displays D-tartrate dehydratase activity in vitro, but is not able to convert (R)-malate, L-tartrate or meso-tartrate. Can also catalyze the isomerization of enol- to keto-oxaloacetate.By similarity

Catalytic activityi

(S)-malate = fumarate + H2O.By similarity
Keto-oxaloacetate = enol-oxaloacetate.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class I, aerobic (fumA), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase
Biological processTricarboxylic acid cycle
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class I, aerobicBy similarity (EC:4.2.1.2By similarity)
Alternative name(s):
Fumarase ABy similarity
Oxaloacetate keto--enol-isomeraseBy similarity
Short name:
OAAKE isomeraseBy similarity
Oxaloacetate tautomeraseBy similarity (EC:5.3.2.2By similarity)
Gene namesi
Name:fumABy similarity
Ordered Locus Names:Z2615, ECs2318
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001956572 – 548Fumarate hydratase class I, aerobicAdd BLAST547

Expressioni

Inductioni

Is expressed under aerobic conditions. Is repressed by glucose and anaerobiosis.By similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z2615

Structurei

3D structure databases

ProteinModelPortaliQ8X4P8
SMRiQ8X4P8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I fumarase family.Curated

Phylogenomic databases

eggNOGiENOG4107GFK Bacteria
COG1838 LUCA
COG1951 LUCA
HOGENOMiHOG000009338
KOiK01676
OMAiNKSKMVM

Family and domain databases

Gene3Di3.20.130.10, 1 hit
InterProiView protein in InterPro
IPR004646 Fe-S_hydro-lyase_TtdA-typ_cat
IPR004647 Fe-S_hydro-lyase_TtdB-typ_cat
IPR036660 Fe-S_hydroAse_TtdB_cat_sf
IPR011167 Fe_dep_fumarate_hydratase
IPR020557 Fumarate_lyase_CS
PfamiView protein in Pfam
PF05681 Fumerase, 1 hit
PF05683 Fumerase_C, 1 hit
PIRSFiPIRSF001394 Fe_dep_fumar_hy, 1 hit
SUPFAMiSSF117457 SSF117457, 1 hit
TIGRFAMsiTIGR00722 ttdA_fumA_fumB, 1 hit
TIGR00723 ttdB_fumA_fumB, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8X4P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS LSEFEGQEIL KVAPEALTLL
60 70 80 90 100
ARQAFHDASF MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG
110 120 130 140 150
VLPTCQDTGT AIIVGKKGQR VWTGGGDEAA LARGVYNTYI EDNLRYSQNA
160 170 180 190 200
PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCIAKGGGSA NKTYLYQETK
210 220 230 240 250
ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA
260 270 280 290 300
KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV
310 320 330 340 350
IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL
360 370 380 390 400
RKAGEGEAVR VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL
410 420 430 440 450
KERMDNGEGL PQYIKDHPIY YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ
460 470 480 490 500
LQALGGSMIM LAKGNRSQQV TDACKKHGGF YLGSIGGPAA VLAQGSIKSL
510 520 530 540
ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK
Length:548
Mass (Da):60,298
Last modified:January 23, 2007 - v3
Checksum:iF26C7AA07630563F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG56599.1
BA000007 Genomic DNA Translation: BAB35741.1
PIRiC85767
F90918
RefSeqiNP_310345.1, NC_002695.1
WP_000066619.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG56599; AAG56599; Z2615
BAB35741; BAB35741; BAB35741
GeneIDi916929
KEGGiece:Z2615
ecs:ECs2318
PATRICifig|386585.9.peg.2428

Similar proteinsi

Entry informationi

Entry nameiFUMA_ECO57
AccessioniPrimary (citable) accession number: Q8X4P8
Secondary accession number(s): Q7ADM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 102 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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