Reviewed, UniProtKB/Swiss-Prot Q8X4P8 (FUMA_ECO57)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class I, aerobic
      Short name=Fumarase
    EC=4.2.1.2

Gene names

Name:	fumA
Ordered Locus Names:	Z2615, ECs2318

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically By similarity.
Catalytic activity	(S)-malate = fumarate + H2O.
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Enzyme regulation	Subject to aerobic respiratory control and catabolite repression.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the class-I fumarase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW fumarate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 548	547	Fumarate hydratase class I, aerobic		PRO_0000195657
Sites
	Active site	397	1	Potential
	Metal binding	318	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	463	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8X4P8-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F26C7AA07630563F	FASTA	548	60,298
	10 20 30 40 50 60 MSNKPFHYQA PFPLKKDDTE YYLLTSEHVS LSEFEGQEIL KVAPEALTLL ARQAFHDASF 70 80 90 100 110 120 MLRPAHQQQV ADILRDPEAS ENDKYVALQF LRNSDIAAKG VLPTCQDTGT AIIVGKKGQR 130 140 150 160 170 180 VWTGGGDEAA LARGVYNTYI EDNLRYSQNA PLDMYKEVNT GTNLPAQIDL YAVDGDEYKF 190 200 210 220 230 240 LCIAKGGGSA NKTYLYQETK ALLTPGKLKN YLVEKMRTLG TAACPPYHIA FVIGGTSAET 250 260 270 280 290 300 NLKTVKLASA KYYDELPTEG NEHGQAFRDV ELEKELLIEA QNLGLGAQFG GKYFAHDIRV 310 320 330 340 350 360 IRLPRHGASC PVGMGVSCSA DRNIKAKINR QGIWIEKLEH NPGKYIPEEL RKAGEGEAVR 370 380 390 400 410 420 VDLNRPMKEI LAQLSQYPVS TRLSLNGTII VGRDIAHAKL KERMDNGEGL PQYIKDHPIY 430 440 450 460 470 480 YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ LQALGGSMIM LAKGNRSQQV TDACKKHGGF 490 500 510 520 530 540 YLGSIGGPAA VLAQGSIKSL ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQL TQCTRCVK

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG56599.1. BA000007 Genomic DNA. Translation: BAB35741.1.
PIR	C85767. F90918.
RefSeq	NP_288047.1. NP_310345.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	916929. 961562.
GenomeReviews	Gene locus Z2615 in contig AE005174_GR. Gene locus ECs2318 in contig BA000007_GR.
KEGG	ece:Z2615. ecs:ECs2318.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8X4P8.
OMA	FQQIQTS.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2318-MON.
Family and domain databases
InterPro	IPR011167. Fe_dep_fumarate_hydratase. IPR020557. Fumarate_lyase_CS. IPR004646. TtdA_fumA_fumB. IPR004647. TtdB_fumA_fumB. [Graphical view]
Gene3D	G3DSA:3.20.130.10. TtdB_fumA_fumB. 1 hit.
Pfam	PF05681. Fumerase. 1 hit. PF05683. Fumerase_C. 1 hit. [Graphical view]
PIRSF	PIRSF001394. Fe_dep_fumar_hy. 1 hit.
TIGRFAMs	TIGR00722. ttdA_fumA_fumB. 1 hit. TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMA_ECO57

Accession

Primary (citable) accession number: Q8X4P8
Secondary accession number(s): Q7ADM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)