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Q8X4I9

- ALR2_ECO57

UniProt

Q8X4I9 - ALR2_ECO57

Protein

Alanine racemase, catabolic

Gene

dadX

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (05 Mar 2002)
      Previous versions | rss
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    Functioni

    Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.By similarity

    Catalytic activityi

    L-alanine = D-alanine.

    Cofactori

    Pyridoxal phosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei35 – 351Proton acceptor; specific for D-alanineBy similarity
    Binding sitei130 – 1301SubstrateBy similarity
    Active sitei253 – 2531Proton acceptor; specific for L-alanineBy similarity
    Binding sitei301 – 3011Substrate; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. alanine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-1667-MONOMER.
    ECOO157:DADX-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemase, catabolic (EC:5.1.1.1)
    Gene namesi
    Name:dadX
    Synonyms:dadB
    Ordered Locus Names:Z1953, ECs1685
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Alanine racemase, catabolicPRO_0000114519Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei35 – 351N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiQ8X4I9.

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z1953.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X4I9.
    SMRiQ8X4I9. Positions 3-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.Curated

    Phylogenomic databases

    eggNOGiCOG0787.
    HOGENOMiHOG000031446.
    KOiK01775.
    OMAiYGHGLER.
    OrthoDBiEOG6PP9NJ.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.
    PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8X4I9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRPIQASLD LQALKQNLSI VRQAAPHARV WSVVKANAYG HGIERIWSAL    50
    GATDGFALLN LEEAITLRER GWKGPILMLE GFFHAQDLEM YDQHRLTTCV 100
    HSNWQLKALQ NARLKAPLDI YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN 150
    VGEMTLMSHF AEAEHPDGIS GAMARIEQAA EGLECRRSLS NSAATLWHPE 200
    AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII GVQTLKAGER 250
    VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS 300
    MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR 350
    VPVVTV 356
    Length:356
    Mass (Da):38,859
    Last modified:March 5, 2002 - v1
    Checksum:i28B67F19517A3B09
    GO

    Sequence cautioni

    The sequence BAB35108.1 differs from that shown. Reason: Frameshift at position 321.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti314 – 3152AG → RD in BAB35108. (PubMed:11258796)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG56041.1.
    BA000007 Genomic DNA. Translation: BAB35108.1. Frameshift.
    PIRiE85697.
    E90839.
    RefSeqiNP_287429.1. NC_002655.2.

    Genome annotation databases

    EnsemblBacteriaiAAG56041; AAG56041; Z1953.
    BAB35108; BAB35108; BAB35108.
    GeneIDi960219.
    KEGGiece:Z1953.
    PATRICi18352622. VBIEscCol44059_1536.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG56041.1 .
    BA000007 Genomic DNA. Translation: BAB35108.1 . Frameshift.
    PIRi E85697.
    E90839.
    RefSeqi NP_287429.1. NC_002655.2.

    3D structure databases

    ProteinModelPortali Q8X4I9.
    SMRi Q8X4I9. Positions 3-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z1953.

    Proteomic databases

    PRIDEi Q8X4I9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG56041 ; AAG56041 ; Z1953 .
    BAB35108 ; BAB35108 ; BAB35108 .
    GeneIDi 960219.
    KEGGi ece:Z1953.
    PATRICi 18352622. VBIEscCol44059_1536.

    Phylogenomic databases

    eggNOGi COG0787.
    HOGENOMi HOG000031446.
    KOi K01775.
    OMAi YGHGLER.
    OrthoDBi EOG6PP9NJ.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-1667-MONOMER.
    ECOO157:DADX-MONOMER.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiALR2_ECO57
    AccessioniPrimary (citable) accession number: Q8X4I9
    Secondary accession number(s): Q8X2W4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3