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Q8X4I9

- ALR2_ECO57

UniProt

Q8X4I9 - ALR2_ECO57

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Protein

Alanine racemase, catabolic

Gene

dadX

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.By similarity

Catalytic activityi

L-alanine = D-alanine.

Cofactori

Pyridoxal phosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351Proton acceptor; specific for D-alanineBy similarity
Binding sitei130 – 1301SubstrateBy similarity
Active sitei253 – 2531Proton acceptor; specific for L-alanineBy similarity
Binding sitei301 – 3011Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciECOL386585:GJFA-1667-MONOMER.
ECOO157:DADX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, catabolic (EC:5.1.1.1)
Gene namesi
Name:dadX
Synonyms:dadB
Ordered Locus Names:Z1953, ECs1685
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Alanine racemase, catabolicPRO_0000114519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PRIDEiQ8X4I9.

Interactioni

Protein-protein interaction databases

STRINGi155864.Z1953.

Structurei

3D structure databases

ProteinModelPortaliQ8X4I9.
SMRiQ8X4I9. Positions 3-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.Curated

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031446.
KOiK01775.
OMAiYGHGLER.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8X4I9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRPIQASLD LQALKQNLSI VRQAAPHARV WSVVKANAYG HGIERIWSAL
60 70 80 90 100
GATDGFALLN LEEAITLRER GWKGPILMLE GFFHAQDLEM YDQHRLTTCV
110 120 130 140 150
HSNWQLKALQ NARLKAPLDI YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN
160 170 180 190 200
VGEMTLMSHF AEAEHPDGIS GAMARIEQAA EGLECRRSLS NSAATLWHPE
210 220 230 240 250
AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII GVQTLKAGER
260 270 280 290 300
VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS
310 320 330 340 350
MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR

VPVVTV
Length:356
Mass (Da):38,859
Last modified:March 5, 2002 - v1
Checksum:i28B67F19517A3B09
GO

Sequence cautioni

The sequence BAB35108.1 differs from that shown. Reason: Frameshift at position 321.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti314 – 3152AG → RD in BAB35108. (PubMed:11258796)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56041.1.
BA000007 Genomic DNA. Translation: BAB35108.1. Frameshift.
PIRiE85697.
E90839.
RefSeqiNP_287429.1. NC_002655.2.

Genome annotation databases

EnsemblBacteriaiAAG56041; AAG56041; Z1953.
BAB35108; BAB35108; BAB35108.
GeneIDi960219.
KEGGiece:Z1953.
PATRICi18352622. VBIEscCol44059_1536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56041.1 .
BA000007 Genomic DNA. Translation: BAB35108.1 . Frameshift.
PIRi E85697.
E90839.
RefSeqi NP_287429.1. NC_002655.2.

3D structure databases

ProteinModelPortali Q8X4I9.
SMRi Q8X4I9. Positions 3-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z1953.

Proteomic databases

PRIDEi Q8X4I9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG56041 ; AAG56041 ; Z1953 .
BAB35108 ; BAB35108 ; BAB35108 .
GeneIDi 960219.
KEGGi ece:Z1953.
PATRICi 18352622. VBIEscCol44059_1536.

Phylogenomic databases

eggNOGi COG0787.
HOGENOMi HOG000031446.
KOi K01775.
OMAi YGHGLER.
OrthoDBi EOG6PP9NJ.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-1667-MONOMER.
ECOO157:DADX-MONOMER.

Family and domain databases

Gene3Di 2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPi MF_01201. Ala_racemase.
InterProi IPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view ]
Pfami PF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view ]
PRINTSi PR00992. ALARACEMASE.
SMARTi SM01005. Ala_racemase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsi TIGR00492. alr. 1 hit.
PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiALR2_ECO57
AccessioniPrimary (citable) accession number: Q8X4I9
Secondary accession number(s): Q8X2W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: October 1, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3