Q8X4I9 (ALR2_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase, catabolic
EC=5.1.1.1

Gene names

Name:	dadX
Synonyms:	dadB
Ordered Locus Names:	Z1953, ECs1685

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA By similarity. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Sequence caution	The sequence BAB35108.1 differs from that shown. Reason: Frameshift at position 321.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	alanine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Alanine racemase, catabolic HAMAP-Rule MF_01201		PRO_0000114519
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	253	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	130	1	Substrate By similarity
	Binding site	301	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	314 – 315	2	AG → RD in BAB35108. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8X4I9 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 28B67F19517A3B09	FASTA	356	38,859
	10 20 30 40 50 60 MTRPIQASLD LQALKQNLSI VRQAAPHARV WSVVKANAYG HGIERIWSAL GATDGFALLN 70 80 90 100 110 120 LEEAITLRER GWKGPILMLE GFFHAQDLEM YDQHRLTTCV HSNWQLKALQ NARLKAPLDI 130 140 150 160 170 180 YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN VGEMTLMSHF AEAEHPDGIS GAMARIEQAA 190 200 210 220 230 240 EGLECRRSLS NSAATLWHPE AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII 250 260 270 280 290 300 GVQTLKAGER VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS 310 320 330 340 350 MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR VPVVTV

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56041.1. BA000007 Genomic DNA. Translation: BAB35108.1. Frameshift.
PIR	E85697. E90839.
RefSeq	NP_287429.1. NC_002655.2. NP_309712.1. NC_002695.1.
3D structure databases
ProteinModelPortal	Q8X4I9.
SMR	Q8X4I9. Positions 3-356.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z1953.
Proteomic databases
PRIDE	Q8X4I9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG56041; AAG56041; Z1953. BAB35108; BAB35108; BAB35108.
GeneID	913184. 960219.
KEGG	ece:Z1953. ecs:ECs1685.
PATRIC	18352622. VBIEscCol44059_1536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031446.
KO	K01775.
OMA	GQWQDIA.
ProtClustDB	PRK03646.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-1667-MONOMER.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_ECO57

Accession

Primary (citable) accession number: Q8X4I9
Secondary accession number(s): Q8X2W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program