Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alanine racemase, catabolic

Gene

dadX

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.By similarity

Catalytic activityi

L-alanine = D-alanine.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei35Proton acceptor; specific for D-alanineBy similarity1
Binding sitei130SubstrateBy similarity1
Active sitei253Proton acceptor; specific for L-alanineBy similarity1
Binding sitei301Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, catabolic (EC:5.1.1.1)
Gene namesi
Name:dadX
Synonyms:dadB
Ordered Locus Names:Z1953, ECs1685
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145191 – 356Alanine racemase, catabolicAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Protein-protein interaction databases

STRINGi155864.Z1953

Structurei

3D structure databases

ProteinModelPortaliQ8X4I9
SMRiQ8X4I9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJ4 Bacteria
COG0787 LUCA
HOGENOMiHOG000031446
KOiK01775
OMAiWRGPILL

Family and domain databases

Gene3Di2.40.37.10, 1 hit
3.20.20.10, 1 hit
HAMAPiMF_01201 Ala_racemase, 1 hit
InterProiView protein in InterPro
IPR000821 Ala_racemase
IPR009006 Ala_racemase/Decarboxylase_C
IPR011079 Ala_racemase_C
IPR001608 Ala_racemase_N
IPR020622 Ala_racemase_pyridoxalP-BS
IPR029066 PLP-binding_barrel
PfamiView protein in Pfam
PF00842 Ala_racemase_C, 1 hit
PF01168 Ala_racemase_N, 1 hit
PRINTSiPR00992 ALARACEMASE
SMARTiView protein in SMART
SM01005 Ala_racemase_C, 1 hit
SUPFAMiSSF50621 SSF50621, 1 hit
SSF51419 SSF51419, 1 hit
TIGRFAMsiTIGR00492 alr, 1 hit
PROSITEiView protein in PROSITE
PS00395 ALANINE_RACEMASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8X4I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPIQASLD LQALKQNLSI VRQAAPHARV WSVVKANAYG HGIERIWSAL
60 70 80 90 100
GATDGFALLN LEEAITLRER GWKGPILMLE GFFHAQDLEM YDQHRLTTCV
110 120 130 140 150
HSNWQLKALQ NARLKAPLDI YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN
160 170 180 190 200
VGEMTLMSHF AEAEHPDGIS GAMARIEQAA EGLECRRSLS NSAATLWHPE
210 220 230 240 250
AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII GVQTLKAGER
260 270 280 290 300
VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS
310 320 330 340 350
MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR

VPVVTV
Length:356
Mass (Da):38,859
Last modified:March 5, 2002 - v1
Checksum:i28B67F19517A3B09
GO

Sequence cautioni

The sequence BAB35108 differs from that shown. Reason: Frameshift at position 321.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314 – 315AG → RD in BAB35108 (PubMed:11258796).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG56041.1
BA000007 Genomic DNA Translation: BAB35108.1 Frameshift.
PIRiE85697
E90839
RefSeqiWP_000197859.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG56041; AAG56041; Z1953
BAB35108; BAB35108; BAB35108
KEGGiece:Z1953
PATRICifig|83334.175.peg.4325

Similar proteinsi

Entry informationi

Entry nameiALR2_ECO57
AccessioniPrimary (citable) accession number: Q8X4I9
Secondary accession number(s): Q8X2W4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: April 25, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health