Q8X239 (GUAAA_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Synonyms:	guaA
Ordered Locus Names:	Saci_0548

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.
Sequence caution	The sequence CAC80124.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140232
Regions
	Domain	2 – 188	187	Glutamine amidotransferase type-1
Sites
	Active site	79	1	Nucleophile By similarity
	Active site	166	1	By similarity
	Active site	168	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8X239 [UniParc]. Last modified August 30, 2005. Version 3. Checksum: 4215A86117773EB7	FASTA	188	20,858
	10 20 30 40 50 60 MKVAVIYFGG QYNHLIVKDL KYLGLEAVAI TPDKSVEELK EFDSVVFGGG PYSVINELDK 70 80 90 100 110 120 MGFAPDYVKS LNVPKLGICL GHQLIAKVLG GEVRKANKPE YGLTTVNIVD EDTILRGLKP 130 140 150 160 170 180 SIKAWESHND EVVRPPSGFR ILASSENAKV QAMVNNDNSI FGVQFHPEVK HTEKGIEVFK NFIKICRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]	"Genomic sequence coding for putative GMP synthase and quinolate phosphoribosyltransferase from Sulfolobus acidocaldarius." Komorowski L., Schaefer G. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-188. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY79944.1. AJ132638 Genomic DNA. Translation: CAC80124.1. Different initiation.
RefSeq	YP_255237.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q8X239.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3474610.
GenomeReviews	Gene locus Saci_0548 in contig CP000077_GR.
KEGG	sai:Saci_0548.
NMPDR	fig|330779.3.peg.1792.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG292341.
OMA	GQYVHRI.
PhylomeDB	Q8X239.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_0548-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_SULAC

Accession

Primary (citable) accession number: Q8X239
Secondary accession number(s): Q4JB85

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	August 30, 2005
Last modified:	January 25, 2012
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program