Catalase-peroxidase - Blumeria graminis

Preferred order of sections

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Synonyms:	CPX

Organism

Blumeria graminis

Taxonomic identifier

34373 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Erysiphales › Erysiphaceae › Blumeria

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O. 2 H₂O₂ = O₂ + 2 H₂O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.
Sequence caution	The sequence AAL56991.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 730

730

Catalase-peroxidase

PRO_0000354104

Sites

Active site

93

1

Proton acceptor By similarity

Metal binding

266

1

Iron (heme axial ligand) By similarity

Site

89

1

Transition state stabilizer By similarity

Amino acid modifications

Cross-link

92 ↔ 225

Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity

Cross-link

225 ↔ 251

Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-92) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8X1N3 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: 7C547BA50B90F4F7
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FASTA

730

81,024

        10         20         30         40         50         60 
MGDSKCPYRD ANVAGGGTHN KDWWPETLKL DALRQHTAES NPLGKDFDYA SAFKTLDYEG 

        70         80         90        100        110        120 
LKKDLKDLMT DSQDWWPADF GHYGGFFVRM AWHSAGTYRS IDGRGGGGQG QHRFAPLNSW 

       130        140        150        160        170        180 
PDNGNLDKAR RLLWPIKQKY GNKISWADLY LLTGNVAIES MGGKTFGFAC GRPDTWEADD 

       190        200        210        220        230        240 
ATFWGNETKW LGNDARYKNG SKDPKDIYTR QLEKPLSAVH MGLIYVNPEG PDGIPDPVAS 

       250        260        270        280        290        300 
ARDIRTTFRR MAMNDEETVA LIAGGHTFGK THGAAPATHL GKEPEGAPIE AQGLGWANSY 

       310        320        330        340        350        360 
RSGKGPDTIT SGLEVIWTKT PINWSNHYLE YLFKYDWELT KSPGGANQWT AKNAEAFIPD 

       370        380        390        400        410        420 
AFDPNKKHPP RMLTTDLALR HDKEYEKISL RFLENPDQFA DAFARAWFKL LHRDMGPRSR 

       430        440        450        460        470        480 
WLGPEIPKEE LIWEDPIPEI DHPIISQEDI NNLKKEILSS GVGHNKLIQT AWASASTFRG 

       490        500        510        520        530        540 
GDKRGGANGA RIRLAPQKDW KVNNPPQLTC VLETLGKIQS SFNSSQTGGK IVSLADLIIL 

       550        560        570        580        590        600 
AGCAALEKAA GVPVPFSPGR ADASQEQTDI KSFSNLEPVA DGFRNFGRST PRARAEHMLV 

       610        620        630        640        650        660 
DRAQLLTLTP PELTALVGGL RVLDTNFDGS SCGVFTKRPG QLTNDFFVNL LDPAISWKGI 

       670        680        690        700        710        720 
DEDEFFEGID RKTDEKKWIG SRADLVFGSQ AELRAIAEVY GSADGNEKLI KDFIAAWNKV 

       730 
MNLDLFNLAH

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References

[1]	"Blumeria graminis catalase/peroxidase plays roles in scavenging activated oxygen species in oxidative burst during barley-powdery mildew interactions." Zhang Z., Gurr S.J. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF329396 Genomic DNA. Translation: AAL56991.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ModBase	Search...
Protein family/group databases
PeroxiBase	1960. BgCP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BLUGR

Accession

Primary (citable) accession number: Q8X1N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	October 19, 2011
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families