Q8X1D8 (Q8X1D8_FUSOX) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Nitroalkane oxidase EMBL AAL57485.1
Organism	Fusarium oxysporum (Panama disease fungus) EMBL AAL57485.1
Taxonomic identifier	5507 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family. RuleBase RU004309

Ontologies

Keywords
Ligand	FAD RuleBase RU003714 PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ Flavoprotein RuleBase RU003714 PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ Nucleotide-binding PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
Molecular function	Oxidoreductase RuleBase RU003714
Technical term	3D-structure PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
Gene Ontology (GO)
Molecular function	acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	131 – 134	4	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
	Nucleotide binding	139 – 141	3	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
	Nucleotide binding	169 – 171	3	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
	Nucleotide binding	313 – 314	2	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
	Nucleotide binding	375 – 379	5	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
	Nucleotide binding	400 – 404	5	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ
Sites
	Binding site	304	1	FAD PDB 2C0U PDB 2C12 PDB 2REH PDB 2ZAF PDB 3D9D PDB 3D9E PDB 3D9F PDB 3D9G PDB 3FCJ

Sequences

	Sequence		Length	Mass (Da)
	Q8X1D8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9F156889F2A5901A	FASTA	439	48,162
	10 20 30 40 50 60 MVDFKLSPSQ LEARRHAQAF ANTVLTKASA EYSTQKDQLS RFQATRPFYR EAVRHGLIKA 70 80 90 100 110 120 QVPIPLGGTM ESLVHESIIL EELFAVEPAT SITIVATALG LMPVILCDSP SLQEKFLKPF 130 140 150 160 170 180 ISGEGEPLAS LMHSEPNGTA NWLQKGGPGL QTTARKVGNE WVISGEKLWP SNSGGWDYKG 190 200 210 220 230 240 ADLACVVCRV SDDPSKPQDP NVDPATQIAV LLVTRETIAN NKKDAYQILG EPELAGHITT 250 260 270 280 290 300 SGPHTRFTEF HVPHENLLCT PGLKAQGLVE TAFAMSAALV GAMAIGTARA AFEEALVFAK 310 320 330 340 350 360 SDTRGGSKHI IEHQSVADKL IDCKIRLETS RLLVWKAVTT LEDEALEWKV KLEMAMQTKI 370 380 390 400 410 420 YTTDVAVECV IDAMKAVGMK SYAKDMSFPR LLNEVMCYPL FDGGNIGLRR RQMQRVMALE 430 DYEPWAATYG SSKVDKSRL

References

[1]	"Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily." Daubner S.C., Gadda G., Valley M.P., Fitzpatrick P.F. Proc. Natl. Acad. Sci. U.S.A. 99:2702-2707(2002) [PubMed: 11867731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Daubner C., Gadda G., Fitzpatrick P.F. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase." Fitzpatrick P.F., Bozinovski D.M., Heroux A., Shaw P.G., Valley M.P., Orville A.M. Biochemistry 46:13800-13808(2007) [PubMed: 17994768] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH FAD.
[4]	"Crystal structures of intermediates in the nitroalkane oxidase reaction." Heroux A., Bozinovski D.M., Valley M.P., Fitzpatrick P.F., Orville A.M. Biochemistry 48:3407-3416(2009) [PubMed: 19265437] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 2-439 IN COMPLEX WITH FAD.
[5]	"Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover." Nagpal A., Valley M.P., Fitzpatrick P.F., Orville A.M. Biochemistry 45:1138-1150(2006) [PubMed: 16430210] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.07 ANGSTROMS) IN COMPLEX WITH FAD.
[6]	"Differential quantum tunneling contributions in nitroalkane oxidase catalyzed and the uncatalyzed proton transfer reaction." Major D.T., Heroux A., Orville A.M., Valley M.P., Fitzpatrick P.F., Gao J. Proc. Natl. Acad. Sci. U.S.A. 106:20734-20739(2009) [PubMed: 19926855] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 2-439 IN COMPLEX WITH FAD.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF425595 mRNA. Translation: AAL57485.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C0U	X-ray	2.20	A/B/C/D	1-439	[»]
2C12	X-ray	2.07	A/B/C/D/E/F	1-439	[»]
2REH	X-ray	2.40	A/B/C/D	1-439	[»]
2ZAF	X-ray	2.50	A/B/C/D	1-439	[»]
3D9D	X-ray	2.10	A/B/C/D	2-439	[»]
3D9E	X-ray	2.20	A/B/C/D	2-439	[»]
3D9F	X-ray	2.20	A/B/C/D	2-439	[»]
3D9G	X-ray	2.15	A/B/C/D	2-439	[»]
3FCJ	X-ray	2.40	A/B/C/D	2-439	[»]

ProteinModelPortal

Q8X1D8.

SMR

Q8X1D8. Positions 2-431.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR006092. Acyl-CoA_DH_N.
IPR006090. Acyl-CoA_Oxase/DH_1.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase.
[Graphical view]

Gene3D

G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit.
G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit.
G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.

Pfam

PF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]

SUPFAM

SSF56645. AcylCoA_dehyd_NM. 1 hit.
SSF47203. AcylCoADH_C_like. 1 hit.

ProtoNet

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Entry information

Entry name

Q8X1D8_FUSOX

Accession

Primary (citable) accession number: Q8X1D8

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2002
Last sequence update:	March 1, 2002
Last modified:	July 27, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)