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Protein

Catalase-peroxidase

Gene

katG

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per monomer.UniRule annotation

Enzyme regulationi

Inhibited by KCN.1 Publication

Kineticsi

  1. KM=13.2 mM for H2O2 for the catalase reaction1 Publication

    pH dependencei

    Optimum pH is 4.75 for the peroxidase reaction and 6.25 for the catalase reaction.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei87 – 871Transition state stabilizerUniRule annotation
    Active sitei91 – 911Proton acceptorUniRule annotation
    Metal bindingi279 – 2791Iron (heme axial ligand)UniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Hydrogen peroxide

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.11.1.6. 3627.

    Protein family/group databases

    PeroxiBasei2181. NcCP01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
    Short name:
    CPUniRule annotation
    Alternative name(s):
    Catalase-2
    Peroxidase/catalaseUniRule annotation
    Gene namesi
    Name:katGUniRule annotation
    Synonyms:cat-2
    ORF Names:NCU05770
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    Proteomesi
    • UP000001805 Componenti: Chromosome 7, Linkage Group VII

    Organism-specific databases

    EuPathDBiFungiDB:NCU05770.

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 753753Catalase-peroxidasePRO_0000055582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki90 ↔ 238Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-264)UniRule annotation
    Cross-linki238 ↔ 264Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-90)UniRule annotation

    Post-translational modificationi

    The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

    Proteomic databases

    PRIDEiQ8X182.

    Expressioni

    Inductioni

    Induced in late stationary growth phase.1 Publication

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X182.
    SMRiQ8X182. Positions 15-741.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000218110.
    InParanoidiQ8X182.
    KOiK03782.
    OMAiTESKCPF.
    OrthoDBiEOG7BKD32.

    Family and domain databases

    HAMAPiMF_01961. Catal_peroxid.
    InterProiIPR000763. Catalase_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    IPR019793. Peroxidases_heam-ligand_BS.
    [Graphical view]
    PfamiPF00141. peroxidase. 2 hits.
    [Graphical view]
    PRINTSiPR00460. BPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 2 hits.
    TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
    PROSITEiPS00435. PEROXIDASE_1. 1 hit.
    PS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8X182-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSECPVRKSN VGGGGTRNHD WWPAQLRLNI LRQHTPVSNP LDKDFDYAAA
    60 70 80 90 100
    FKSLDYEGLK KDLTKLMTDS QDWWPADFGH YGGLFIRMAW HSAGTYRVTD
    110 120 130 140 150
    GRGGGGEGQQ RFAPLNSWPD NVSLDKARRL LWPIKQKYGN KISWSDLLLL
    160 170 180 190 200
    TGNVALESMG FKTFGFAGGR PDTWEADESV YWGAETTWLG NEDRYSEGQE
    210 220 230 240 250
    GHEGHGVVQG DESKKQHTDI HNRDLQSPLA SSHMGLIYVN PEGPDGIPDP
    260 270 280 290 300
    VASAKDIRVT FGRMAMNDEE TVALIAGGHS FGKTHGAGPT HHVGKEPEAA
    310 320 330 340 350
    PIEHQGLGWA NSFGQGKGPD TITSGLEVTW TPTPTKWGMG YLEYLYKFDW
    360 370 380 390 400
    EPTKSPAGAN QWVAKNAEPT IPDAYDPNKK KLPTMLTTDI ALRMDPAYDK
    410 420 430 440 450
    ICRDYLANPD KFADAFARAW FKLLHRDMGP RTRWIGPEVP SEILPWEDYI
    460 470 480 490 500
    PPVDYQIIDD NDIAALKKEI LATGVAPKKL IFVAWSSASS FRGSDKRGGA
    510 520 530 540 550
    NGARIRLAPQ NEWKVNDPST LREVLAALES VQQKFNDSSS GKKVSLADLI
    560 570 580 590 600
    VLGGVAALEQ ASGLVVPFTP GRNDATQEHT DVHSFTHLEP HADGFRSYGK
    610 620 630 640 650
    GTKRVRTEQF LIDRASLLTL SAPELTALIG GLRVLEANYD GSSYGVLTKT
    660 670 680 690 700
    PGKLTNDYFV NLLDTNTAWK AADNEGEVFI GYDRKTHDKK WTATRADLIF
    710 720 730 740 750
    GAHAELRALA EVYAAVDGEE KFKRDFVAAW HKVMNLDRFD LKQEGRGQNA

    PKL
    Length:753
    Mass (Da):83,380
    Last modified:October 1, 2002 - v2
    Checksum:iDED01DC6D10BA582
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF459787 mRNA. Translation: AAL66352.2.
    CM002242 Genomic DNA. Translation: EAA30509.1.
    RefSeqiXP_959745.1. XM_954652.3.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000005784; EFNCRP00000005777; EFNCRG00000005774.
    GeneIDi3875883.
    KEGGincr:NCU05770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF459787 mRNA. Translation: AAL66352.2.
    CM002242 Genomic DNA. Translation: EAA30509.1.
    RefSeqiXP_959745.1. XM_954652.3.

    3D structure databases

    ProteinModelPortaliQ8X182.
    SMRiQ8X182. Positions 15-741.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    PeroxiBasei2181. NcCP01.

    Proteomic databases

    PRIDEiQ8X182.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiEFNCRT00000005784; EFNCRP00000005777; EFNCRG00000005774.
    GeneIDi3875883.
    KEGGincr:NCU05770.

    Organism-specific databases

    EuPathDBiFungiDB:NCU05770.

    Phylogenomic databases

    HOGENOMiHOG000218110.
    InParanoidiQ8X182.
    KOiK03782.
    OMAiTESKCPF.
    OrthoDBiEOG7BKD32.

    Enzyme and pathway databases

    BRENDAi1.11.1.6. 3627.

    Family and domain databases

    HAMAPiMF_01961. Catal_peroxid.
    InterProiIPR000763. Catalase_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    IPR019793. Peroxidases_heam-ligand_BS.
    [Graphical view]
    PfamiPF00141. peroxidase. 2 hits.
    [Graphical view]
    PRINTSiPR00460. BPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 2 hits.
    TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
    PROSITEiPS00435. PEROXIDASE_1. 1 hit.
    PS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Neurospora crassa catalases, singlet oxygen and cell differentiation."
      Peraza L., Hansberg W.
      Biol. Chem. 383:569-575(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 654-670, HEME-BINDING, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, INDUCTION.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiKATG_NEUCR
    AccessioniPrimary (citable) accession number: Q8X182
    Secondary accession number(s): Q7S4Q3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: October 1, 2002
    Last modified: July 6, 2016
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.